Research: Shiraz University of Medical SciencesShiraz University of Medical Sciences · Department of Parasitology and MycologyIran · Shīrāz
Article: The Efficacy of Satureja khuzistanica Essential Oil Treatment in Reducing Escherichia coli O157: H7 Load on Alfalfa Seeds Prior to Sprouting[show abstract] [hide abstract]
ABSTRACT: Along with the popularity of raw sprouts in the food baskets, the outbreaks associated with the consumption of the contaminated sprouts increased. In the present study, the antibacterial effect of the essential oil of Satureja khuzistanica in reducing the Escherichia coli O157:H7 population on alfalfa seeds prior to sprouting was evaluated. The essential oil (EO) was analyzed by gas chromatography/mass spectrometry. Alfalfa seeds were inoculated with E. coli O157:H7. The inoculated seeds were then treated with different concentrations of the essential oil of S. khuzistanica. The surviving populations of E. coli O157:H7 were enumerated by direct plating of the seeds on tryptic soy agar. The oil was rich in carvacrol. Direct surface-plating on selective media showed the treatment resulted in a significant (P ≤ 0.05) reduction of E. coli O157:H7 population in a dose-dependent manner. Increasing the washing time revealed a significant effect on reduction of E. coli O157:H7 population. The considerable antibacterial activity of the oil might be due to the high concentration of carvacrol. As the S. khuzistanica EO did not adversely affect alfalfa seed germination and had a strong antibacterial activity, the oil might be used as a natural antimicrobial product to overcome bacterial contamination of sprouts without compromising the viability.Journal of Food Safety 01/2013; · 0.72 Impact Factor
Article: Synthesis and antifungal activity of benzimidazole, benzotriazole and aminothiazole derivatives.[show abstract] [hide abstract]
ABSTRACT: In recent years, the use of antifungal drugs in human medicine has increased, especially with the advent of AIDS epidemic. Efforts have focused on the development of new, less toxic and more efficacious antifungal drugs with novel mechanism of action. The purpose of this study was to synthesize of some new benzimidazole, benzotriazole and aminothiazole derivatives and to evaluate their activity against some species of Candida, Aspergillus and dermatophytes. The desired compounds were synthesized by the reaction of benzimidazole and benzotriazole with bromoalkanes and also by the reaction of an amide derivative of aminothiazole with 2-piperazino-1-ethanol in an efficient solvent in the presence of tetraethyl ammounim bromide or triethylamine) as catalyst. Chemical structures of all the new compounds were confirmed by spectrophotometric methods. Antifungal activities of the new compounds were evaluated by broth micro dilution method as recommended by CLSI. Among the tested compounds, 1-nonyl-1H-benzo[d]imidazole and 1-decyl-1H-benzo[d]imidazole exhibited the best antifungal activities. Of the examined synthetic compounds in different categories, benzimidazole derivatives established better antifungal activities than benzotriazole derivatives, and the piperazine analogue had no significant antifungal effect.Research in pharmaceutical sciences 04/2012; 7(2):65-72.
Article: Academic Editors: MMohammad Jamal Saharkhiz, Marjan Motamedi, Kamiar Zomorodian, Keyvan Pakshir, Ramin Miri, Kimia Hemyari, V Y B Rios, J Sunderland, Torrado[show abstract] [hide abstract]
ABSTRACT: Variations in quantity and quality of essential oil (EO) from the aerial parts of cultivated Mentha piperita were determined. The EO of air-dried sample was obtained by a hydrodistillation method and analyzed by a gas chromatography/mass spectrometry (GC/MS). The antifungal activity of the EO was investigated by broth microdilution methods as recommended by Clinical and Laboratory Standards Institute. A biofilm formation inhibition was measured by using an XTT reduction assay. Menthol (53.28%) was the major compound of the EO followed by Menthyl acetate (15.1%) and Menthofuran (11.18%). The EO exhibited strong antifungal activities against the examined fungi at concentrations ranging from 0.12 to 8.0 μL/mL. In addition, the EO inhibited the biofilm formation of Candida albicans and C. dubliniensis at concentrations up to 2 μL/mL. Considering the wide range of the antifungal activities of the examined EO, it might be potentially used in the management of fungal infections or in the extension of the shelf life of food products.International Scholarly Research Network. 01/2012;
Article: Chemical Composition and Antimicrobial Activities of Essential Oils from Nepeta cataria L. against Common Causes of Food-Borne Infections.Kamiar Zomorodian, Mohammad Jamal Saharkhiz, Samaneh Shariati, Keyvan Pakshir, Mohammad Javad Rahimi, Reza Khashei[show abstract] [hide abstract]
ABSTRACT: Nepeta cataria L. is traditionally consumed as a food additive. The effects of three different harvest stages of N. cataria essential oils (EOs) against most common causes of food-borne infections were evaluated by broth microdilution method as recommended by the Clinical and Laboratory Standards Institute (CLSI). The chemical composition of the EOs from N. cataria has been analyzed by gas chromatography/mass spectrometry (GC/MS). The analysis of the EOs indicated that 4a-α,7-α,7a-β-nepetalactone (55-58%) and 4a-α,7-β,7a-α-nepetalactone (30-31.2%) were the major compounds of the EOs at all developmental stages. The results showed that the tested EOs exhibited antimicrobial activities against the food-borne pathogens at concentrations of 0.125-2 μL/mL. Based on these results, the EO of N. cataria can possibly be used in food products as a natural preservative agent.ISRN pharmaceutics. 01/2012; 2012:591953.
[show abstract] [hide abstract]
ABSTRACT: Introduction and objective: Dermatophytosis is a common fungal disease which involves the keratinized tissue. Several antifungal agents can be used to manage these infections. Unfortunately, drug resistant can result in treatment failure. The disk diffusion in vitro assay is a simple method that can be used to evaluate antifungal susceptibility in dermatophytes. The main aim of this study was to evaluate the antifungal activity of six antifungal drugs against several fresh clinical dermatophyte Iranian isolates. Materials and methods: Forty clinical dermatophytes were isolated from patients suspected of having active dermatophytosis. Paper disks containing terbinafine, griseofulvin, clotrimazole, miconazole, fluconazole and ketoconazole were used in the disk diffusion method to evaluate the in vitro activity of the antifungal agents by measuring the mean diameter of inhibition around the disks. Results: The isolates belong to three genera and eight species as: Trichophyton mentagrophytes 13(32.5%), T. rubrum 8(20%), Epidermophyton floccosum 7(17.5%), T. violaceum 4(10%), Microsporum gypseum 3(7.5%), T. tonsurans 2(5%), T. verrucosum 2(5%), T. schoenleinii 1(2.5%), and an unknown dermatophyte 1(2.5%). No isolates were resistant to clotrimazole and miconazole. Conclusion: This study revealed that clotrimazole, miconazole, terbinafine, and griseofulvin were the most ideal antifungal drugs for the treatment of dermatophytosis. Disk diffusion method is a simple and valuable method for the evaluation of antifungal susceptibility of dermatophytes.Jundishapur Journal of Microbiology. 01/2009;