Publications

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    Kadry Abdel Khalik, Magdy Abd El-Twab, Rasha Galal
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    ABSTRACT: Genetic diversity and phylogenetic analyses of 24 species, representing nine sections of the genus Galium (Rubiaceae), have been made using the Inter Simple Sequence Repeats (ISSR), Randomly Amplified Polymorphic DNA (RAPD), and combined ISSR and RAPD markers. Four ISSR primers and three RAPD primers generated 250 polymorphic amplified fragments. The results of this study showed that the level of genetic variation in Galium is relatively high. RAPD markers revealed a higher level of polymorphism (158 bands) than ISSR (92 bands). Clustering of genotypes within groups was not similar when RAPD and ISSR derived dendrograms were compared. Six clades can be recognized within Galium, which mostly corroborate, but also partly contradict, traditional groupings. UPGMA-based dendrogram showed a close relationship between members of section Leiogalium with G. verum and G. humifusum (sect. Galium), and G. angustifolium (sect. Lophogalium). Principal coordinated analysis, however, showed some minor differences with UPGMA-based dendrograms. The more apomorphic groups of Galium form the section Leiogalium clade including the perennial sections Galium, Lophogalium, Jubogalium, Hylaea and Leptogalium as well as the annual section Kolgyda. The remaining taxa of Galium are monophyletic.
    Biologia 01/2014; 69(3):300-310. · 0.51 Impact Factor
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    Nasr M. Hassan, Kadry N. Abdel Khalik
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    ABSTRACT: Seed morphology of 29 taxa including 9 subgenera belonging to the genus Veronica was compared using scanning electron microscopy to assess their diagnostic value for systematic studies. Subgenus Beccabunga is the largest in this study represented by 9 taxa. Seed surface often varies from ridged reticulate or verrucose/granulate reticulate to rugose-reticulate. Seed color and size have limited taxonomic significance as their variation is uninformative. Thereagainst, seed shape and seed coat ornamentation present informative characters that can be used efficiently in distinguishing the studied taxa. Our seed features support the DNA sequence data in showing close relationships between V. biloba and V. campylopoda, and between V. fruticans and V. fruticulosa. The present study indicates that V. anagallis-aquatica, V. anagalloides, V. comosa, and V. catenata have more or less the same seed features, consequently, these four former species are most likely representing a single species as reported by earlier studies. Furthermore, V. polita and V. persica are very similar in terms of seed characteristics. Our results justify placement of V. peregina within subgenus Beccabunga and support the monophyly of the subgenus Veronica.
    Journal of Systematics and Evolution 01/2014; 52(2):215–230. · 1.85 Impact Factor
  • Kadry Abdel Khalik
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    ABSTRACT: Genetic diversity and phylogenetic analyses of 24 species, representing nine sections of the genus Galium (Rubiaceae), have been made using the Inter Simple Sequence Repeats (ISSR), Randomly Amplified Polymorphic DNA (RAPD), and combined ISSR and RAPD markers. Four ISSR primers and three RAPD primers generated 250 polymorphic amplified fragments. The results of this study showed that the level of genetic variation in Galium is relatively high. RAPD markers revealed a higher level of polymorphism (158 bands) than ISSR (92 bands). Clustering of genotypes within groups was not similar when RAPD and ISSR derived dendrograms were compared. Six clades can be recognized within Galium, which mostly corroborate, but also partly contradict, traditional groupings. UPGMA-based dendrogram showed a close relationship between members of section Leiogalium with G. verum and G. humifusum (sect. Galium), and G. angustifolium (sect. Lophogalium). Principal coordinated analysis, however, showed some minor differences with UPGMA-based dendrograms. The more apomorphic groups of Galium form the section Leiogalium clade including the perennial sections Galium, Lophogalium, Jubogalium, Hylaea and Leptogalium as well as the annual section Kolgyda. The remaining taxa of Galium are monophyletic.
    Biologia - Section Botany 01/2014; 69(3):300-310.
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    Kadry Abdel Khalik, Iman Hussien Al Gohary
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    ABSTRACT: The seed morphology and seed proteins of 11 Vicia taxa from Egypt were studied. Macro- and micro-morphological characters, including seed shape, color, size, hilum shape and seed sculpture were described and analyzed. The secondary sculpture of the cell wall varies from papillose in nearly all Vicia species to lophate in V. hirsuta. Further, the seed proteins of studied taxa were investigated by SDS-PAGE and 39 different bands were scored. The similarity analysis based on the SDS-PAGE profile and seed morphology was found to be a useful characteristic for the discrimination of Vicia species both on the subgeneric and the sectional levels. The present study did not acknowledge V. tetrasperma and V. hirsuta as subgenus Ervum. As a result, division of Vicia into two subgenera i.e. Vicia and Cracca, is supported.
    Acta Scientiarum Biological Sciences 12/2013; 35(4):603-611.
  • Kadry Abdel Khalik
    Doga, Turkish Journal of Botany 01/2013;
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    Kadry Abdel Khalik
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    ABSTRACT: Seed coat morphology of 15 species of Ipomoea L. was examined comparatively using 2 scanning and light microscopy methods in order to evaluate their diagnostic value for 3 systematic studies. Macro- micro morphological characters, including seed shape, color, 4 size, seed surface, epidermal cell shape, anticlinal boundaries, and periclinal cell wall 5 are presented. Descriptions of seed size, shape, color, surface and seed coat types are 6 summarized for the genus. Taxonomic phylogenetic implications of the seed coat micro 7 morphology are also discussed, in comparison with the available gross morphological 8 and molecular data. Results of the seed character analyses offer useful data evaluating 9 the taxonomy of Ipomoea both on the subgeneric and sectional level. Monophyly of 10 both section Erpipomoea Choisy and Eriospermum Hallier is not supported. A key for 11 the identification of the investigated taxa based on seed characters is provided.
    Turkish Journal of Botany 01/2013; · 1.60 Impact Factor
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    Kadry ABDEL KHALIK a, Mohamed EL-SHEIKH c, Abeer EL-AIDAROUS a
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    ABSTRACT: Wadi Noman in Mecca is one of the most important wadis. It was included in the most important water sources where the spring and wells of Zobida were running. It was providing the holy places in Mecca with drinking water for visitors of Kaaba and Arafat regions. The present study provides an analysis of floristic composition, vegetation types as well as structure and species distribution of 20 sites, emphases on the environmental factors that affect the species distribution. A total of 126 species representing 39 families of the vascular plants are recorded. Fabaceae, Poaceae and Boraginaceae are the largest families. Therophytes and chamaephytes are the most frequent, indicating a typical desert life form spectrum. Floristic composition in the different geomorphologic landscape units showed differences in species richness. The highest species richness of (23 ssp. stand-1) is recorded in the wadi bed. The lowest species richness value (18 ssp. stand-1) is recorded in the wadi plateau and fissures. Chorological analysis revealed that 52% of the studied species are biregional, being native to the Saharo Arabian-Sudano Zambezian region. Four vegetation groups were identified after application of TWINISPAN, DCA and CCA programs and named after the characteristic species as follows: (I) 2 Aristolochia bracteolata-Cucumis prophetarum; (II) Calotropis procera-Acacia hamulosa-Caralluma russeliana; (III) Acacia abyssinica-Acacia hamulosa-Tephrosia desertorum and (IV) Argemone ochroleuca-Senna italica. These plants associations and speciation of Wadi Noman demonstrate significant variation in pH, EC, soil mineral contents and human impacts.
    Turkish Journal of Botany 01/2013; · 1.60 Impact Factor
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    Kadry Abdel Khalik
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    ABSTRACT: A numerical taxonomic study of the family Zygophyllaceae from Egypt). A systematic study of 29 taxa belonging to 7 genera of subfamilies Balanitoideae, Zygophylloideae, Peganoideae, Tribuloideae, Seetzenioideae and Tetradiclidoideae of Zygophyllaceae from Egypt was carried out by means of numerical analysis based on sixty-one morphological characters, including vegetative parts, pollen grains and seeds. On the basis of UPGMA clustering and PCO analysis, six main groups are recognized. Representatives of these groups are clustered together based on characters with high factor loadings in the PCO analysis. Th e results indicate that Zygophyllaceae are heterogeneous, including Peganum harmala which has been proposed to belong in a separate family. Zygophylloideae, Tribuloideae, Tetradiclidoideae and Seetzenioideae are the most homogeneous groups. Sections and groups in both Tribulus and Fagonia seem artifi cial.
    Acta Botanica Brasilica 01/2012; 26(1):165-180. · 0.37 Impact Factor
  • Kadry Abdel Khalik
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    ABSTRACT: To elucidate the genetic diversity of ten Ipomoea species, seed proteins were analyzed by SDS-PAGE and RAPD analysis. According to SDS-PAGE analysis, in total 51 different bands were identified across the studied species. The number of bands varies from 16 bands in Ipomoea cairica, to 3 bands in I. sinensis. The similarity analysis based on the SDS-PAGE profile turned out to be a useful character for the inequity of Ipomoea species both on the subgeneric and sectional level. Analysis of RAPD-PCR of DNA provided more precise information concerning relationships between Ipomoea sections than SDS-PAGE analysis. A remarkable result from this study was identifying a close relationship between Ipomoea purpurea of section Pharbitis (subgenus Ipomoea) and species of the subgenus Quamoclit. Further support comes from the molecular data of RAPD which indicate that Ipomoea cairica should be considered a well separated section which may be related to section Orthipomoea. Our results suggest that section Erpipomoea is not a monophyletic group, whereas species of section Orthipomoea form a single monophyletic section.
    Australian Journal of Crop Science 01/2012; 6(6):1088-1093. · 1.63 Impact Factor
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    Sami Rabei & Kadry Abdel Khalik
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    ABSTRACT: Three keys to 44 species belonging to the family Convolvulaceae in the flora of Egypt are prepared to facilitate the identification of those species, based on 72 characters. These keys were constructed using the DELTA key-generating programs. The first key was built using all characters and the second key was built using 56 characters (vegetative, floral and fruit characters). The first key is considered as main key and can be confirmed by the second key in the field. The third one was built using 16 characters (pollen grains, seed and cell wall characters) which are to be used to identify and confirm the specimen in the laboratory
    Flora Mediterranea. 01/2012; 22:45-62.
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    K. Abdel Khalik and N. M. S. Hassan
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    ABSTRACT: Seed and trichome morphology of 13 taxa representing the genus Fagonia in Egypt have been studied using light (LM) and scanning electron microscopy (SEM). Macro- and micro-morphological characters, including seed shape, seed size, seed coat sculpture, trichome density and structure were studied to evaluate their systematic signifi cance. Seed shape and trichome density were found to have only minor taxonomic value, but seed coat sculpturing and trichome structure proved to be valuable characters. Th ese characters off er evidence to combine certain species, e.g. Fagonia kassasii and F. schimperi in F. bruguieri , Fagonia microphylla in F. scabra and Fagonia thebaica in F. arabica . However, F. sinaica , F. tristis, F. boveana and F. isotricha should be maintained as separate species. Generally, the diff erent patterns of seed and trichome morphology are useful in delimitation of species within the genus Fagonia , but they could not be used to characterize groups of related species.
    Nordic Journal of Botany 01/2012; 30:116-126. · 0.60 Impact Factor
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    A. A. El-Khatib, K. N. abdel-Khalek, Naglaa Usef
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    ABSTRACT: atmospheric pollution biomonitoring anatomy
    Egytian Journal of botany. 01/2011; 51(1):1-12.
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    Kadry Abdel Khalik Assoc. Prof. Dr
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    ABSTRACT: Pollen morphology of nine species belonging to four genera: Cephalaria, Pterocephalus, Scabiosa, and Lomelosia of Dipsacaceae in Egypt has been investigated by light microscope (LM) and scanning electron microscopy (SEM). Pollen shape, size, exine ornamentation, number and characters of apertures present powerful characters for distinguishing between species. The pollen grains were tricolpate and triporate. Their shapes vary from spheroidal to oblate-spheroidal. Regarding the position of apertures, three types can be recognized viz., circumaperturate, angulaperturate, and planaperturate. Concerning sculpturing of the exine in proximal face, two different pollen types can be distinguished viz., spinulate and gemmate types. The exine ornamentation was found useful to distinguish between closely related genera such as Scabiosa and Lomelosia. A diagnostic key is given for all studied taxa based on palynomorphological characters (© 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
    Feddes Repertorium 08/2010; 121(3‐4):97 - 111.
  • kadry N. abdel Khalik
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    ABSTRACT: The seed morphology of nine taxa of Juncus from Egypt has been investigated using light and scanning electron microscopy, to determine the importance of seed coat features as taxonomic characters. Macro- and micromorphological characters, including seed shape, color, size, seed appendages, epidermal cell shape, anticlinal boundaries, and outer periclinal cell wall and secondary cell wall sculpture are presented. Four types of seed appendages are recognized: (i) seeds with two appendages; (ii) seeds without appendages; (iii) seeds with minutely a piculate at one end; and (iv) seeds with minutely a piculate at both ends. Two types of anticlinal cell wall boundaries, (i) raised-channeled, straight and (ii) raised, straight or sinuous, and three different shapes of outer periclinal cell wall are described: (i) flat; (ii) concave; and (iii) flat to slightly concave. The secondary sculpture of the cell wall varies from striate to microreticulate or reticulate, and smooth to finely folded. Seed characters provide useful data for formulating the taxonomy of Juncus both on the subgeneric and sectional level. A key for the identification of the investigated taxa based on seed characters is provided
    Journal of Systematics and Evolution 01/2010; 48(3):215-223. · 1.85 Impact Factor
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    KADRY ABDEL KHALIK, MONIER ABD EL-GHANI, AHMED EL KORDY
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    ABSTRACT: Fruit (mericarp) and seed morphology of 11 species and two subspecies of Galium were examined with light microscope and scanning electron microscope. Macro-and micro- -morphological characters, including fruit and seed shape, colour, size, surface, epidermal cell shape, anticlinal boundaries, outer periclinal cell wall and relief of outer cell walls, are presented. Four different types ofmericarp surface are described. Three types of anticlinal cell wall boundaries of seed are recognized and three different shapes of outer periclinal cell wall are described. The secondary sculpture of the cell wall varies from micro-papillate to micro-reticulate, and smooth to fine- or coarse-folded. The fruit and seed characteristics could be used for taxonomic identification
    Acta Botanica Croatica 01/2008; 67(1). · 0.44 Impact Factor
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    Kadry N Abdel Khalik, Monier M Abd, El -Ghani, Ahmed El Kordy
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    ABSTRACT: On the basis of fifty morphological characters, including vegetative parts, flowers, fruits, seeds, pollen grains, and anatomical structure, a systematic study of 13 taxa belonging to genus Galium (Rubiaceae) from Egypt was conducted by means of numerical analysis. Four branches and clusters were distinguished. Representatives of these groups were clustered together according to characters with high factor loading in the principal coordinates analysis. The results showed congruence between the UPGMA clustering and principal coordinates analysis in suggesting four groups. There was some degree of similarity among the species of sect. Aparine (Kolgyda). The results indicated also that the sect. Leiogalium (G. mollugo) was a separate group, while Aparine (Kolgyda) was the most heterogeneous one.
    PHYTOLOGIA BALCANICA. 01/2008; 14:245-253.
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    Kadry N. ABDEL-KHALIK, Monier M. ABD EL-GHAN, Ahmed A. ELKORDY
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    ABSTRACT: Transverse stem sections of 11 species and 2 subspecies of the genus Galium L. from Egypt were studied by light microscopy. Epidermal cell shape, cortex, xylem, and pith were of good taxonomic value between the taxa. Four types of stem shape were discerned in the transverse sections. The shape of epidermal cells can be used to distinguish between subspecies of Galium setaceum Lam. The cortex varied in size from wide to very narrow. The pith was wide or narrow, and solid or hollow, and was useful for classifying species into groups. A key for the identification of the investigated taxa based on anatomical characters is provided
    Turkish Journal of Botany 01/2008; 32:353-359. · 1.60 Impact Factor
  • Kadry Abdel Khalik
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    ABSTRACT: A systematic study of 44 taxa belonging to 10 genera of tribes Convolvuleae, Cuscuteae, Cresseae, Dichondreae, Ipomoeeae and Merremieae of the Convolvulaceae from Egypt was carried out by means of a phenetic analysis based on seventy one morphological characters, including vegetative parts, pollen grains and seeds. Based on UPGMA clustering and PCO analysis, five main groups are recognized: Convolvuleae, Cuscuteae, Cresseae, Dichondreae and a mixed group from Ipomoeeae and Merremieae. OUT’s of these groups are clustered together based on characters with high factor loading >0.60 in the PCO analysis. Our results indicate that the tribe Convolvuleae is heterogeneous and the Cuscuteae is homogeneous, and there is some degree of similarity between taxa of Merremieae and Ipomoeeae, and those Cresseae and Dichondreae.
    Flora Mediterranea. 01/2008; 18:65-87.
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    K. Abdel Khalik, M. M. Abd El-Ghani, A. Elkordy
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    ABSTRACT: A pollen morphological investigation of eleven species and one subspecies of the genus Galium L. from Egypt were undertaken by using light microscope (LM) and scanning electron microscope (SEM). Pollen shape, size, exine ornamentation, number of apertures present powerful characters for distinguishing between species. The pollen grains were zonocolpate. The number of colpi ranges from 5 to 10. Their shape varies from prolate spheroidal, oblate spheroidal, spheroidal to suboblate. Three groups can be distinguished based on the size pollen grains. It was found used to distinguish between closely related species G. aparine and G. spurium and between G. tricornutum and G. ceratopodum. Two different types of exine ornamentation were recognized. The ornamentation was found useful to distinguish among closely related species such as Galium aparine and G. spurium. A key for the identification of the investigated taxa based on pollen grains characters is provided. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)Eine palynologische Studie der Gattung Galium L. (Rubiaceae) in Ägypten und ihre Bedeutung für die SystematikDie Pollenmorphologie von elf Arten und einer Unterart der Gattung Galium L. aus Ägypten wurde mittels Licht- und Elektronenmikroskopie untersucht. Pollenform, Größe und Muster der Exine und die Anzahl der Aperturen repräsentieren gute Merkmale zur Unterscheidung der betrachteten Arten. Alle Pollen sind zonocolpat. Die Anzahl der Colpi liegt zwischen 5 bis 10. Ihre äußere Form variiert von prolat über sphäroidal, oblat-sphäroidal, sphäroidal bis suboblat. Auf Grund der Pollengröße lassen sich drei Gruppen unterscheiden. Es zeigte sich, dass diese drei Gruppen genutzt werden können, um die eng verwandten Arten Galium aparine und G. spurium sowie G.tricornatum und G. ceratopodum von einander zu trennen. Zwei Typen der Exine-Muster ließen sich erkennen. Sie sind geeignet um z. B. so eng verwandte Arten wie Galium aparine und G. spurium zu trennen. Ein Schlüssel, der geeignet ist, die untersuchten Taxa auf der Basis ihrer Pollenmerkmale zu bestimmen beschließt die Arbeit.
    Feddes Repertorium 11/2007; 118(7‐8):311 - 326.
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    K. Abdel Khalik, A. K. Osman
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    ABSTRACT: Seed morphology of 31 taxa belong to six genera of Convolvulaceae from Egypt were examined by using light and scanning electron microscopy. Macro- and micromorphological characters, including seed shape, colour, size, surface, epidermal cell shape, anticlinal boundaries, outer periclinal cell wall and relief of outer cell walls, are presented. Three types of basic anticlinal cell wall boundaries and three types of relief outer cell walls are recognized and four different shapes of the outer periclinal cell wall are described. A key for the identification of the investigated taxa based on seed characters is provided. (© 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)Morphologie der Samen einiger Arten der Convolvulaceae aus Ägypten (Bestimmung von Arten und systematische Bedeutung)Unter Anwendung von Licht- und Elektronenmikroskopie wurde die Morphologie der Samen von 31 Arten aus sechs Gattungen der Convolvulaceae untersucht. Berücksichtigt wurden die makro- und mikromorphologischen Merkmale der Samen umfassend ußere Form, Farbe, Größe, Oberflche, Form der Epidermiszellen, antiklinale und periklinale Zellwnde und Relief der ußeren Zellwnde. Drei Typen basaler antiklinaler Zellwnde und drei Typen des Reliefs der ußeren Zellwnde wurden nachgewiesen; ferner werden vier Formen der ußeren periklinalen Zellwnde beschrieben. Ein Schlüssel zur Bestimmung der untersuchten Taxa auf der Basis der Merkmale der Samen wird vorgelegt.
    Feddes Repertorium 04/2007; 118(1‐2):24 - 37.

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