Julie Rodriguez

French National Centre for Scientific Research

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Topics (22) View all

Biology ×

164 Questions

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Chemistry ×

461 Questions

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Cell Biology ×

365 Questions

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Neuroscience ×

531 Questions

68358 Followers

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Stem Cells ×

105 Questions

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T Lymphocytes ×

63 Questions

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Protein Engineering ×

32 Questions

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Dendritic Cells ×

58 Questions

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Questions and Answers (2) View all

Answer added in Nanoencapsulation
15 Can anyone recommend where to get GFP plasmids to transfect mammalian cells?
By Abd ALMONEM Doolaanea · International Islamic University Malaysia

Julie Rodriguez · French National Centre for Scientific Research

Hi ! You can try to buy GFP Plasmids from OZ Biosciences. I'm using their PVectOZ (GFP and LacZ) Plasmid for transfection control in HUVEC. it works r... [more]

Hi ! You can try to buy GFP Plasmids from OZ Biosciences. I'm using their PVectOZ (GFP and LacZ) Plasmid for transfection control in HUVEC. it works really well !!

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Answer added in Methods
40 Transfection of human monocytes
By Bram van der Eerden · Erasmus MC

Julie Rodriguez · French National Centre for Scientific Research

Hi Bram, for transfecting human monocytes and generally suspension cells, you can try a viral approach in combination with ViroMag reagent (Magnetofec... [more]

Hi Bram, for transfecting human monocytes and generally suspension cells, you can try a viral approach in combination with ViroMag reagent (Magnetofection). ViroMag would increase viral infectivity and transduction efficiency in any suspension cells such as PBL and PBMC and ViroMag plus a virus have been successfully used on primary T cell and several citations reports have been published (Sacha JB., Nat Protoc. 2010 ; Sakhdari A., PLoS One. 2012) If you prefer a non viral approach you can try with a lipid based reagent. DreamFect Gold reagent is performant on immortalized Lymphocyte cells such as THP-1 (Pakula R., Glycobiology. 2007; Vanpouille C., J Biol Chem. 2007; Wenzel M., J Immunol. 2012). You can contact me if you need more information.

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