Jürgen Angerer

Publications (356) View all

• Source

  Available from: Holger Martin Koch

  Article: Metabolism of the plasticizer and phthalate substitute diisononyl-cyclohexane-1,2-dicarboxylate (DINCH®) in humans after single oral doses

  HolgerM. Koch, André Schütze, Claudia Pälmke, Jürgen Angerer, Thomas Brüning
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  ABSTRACT: Hexamoll® DINCH® (diisononyl-cyclohexane-1,2-dicarboxylate) is a new high-molecular-weight plasticizer and a phthalate substitute. In this study, the metabolism of DINCH® was investigated by oral dosage of three male volunteers with approximately 50 mg Hexamoll® DINCH® (resulting in individual doses between 0.552 and 0.606 mg/kg body weight). Their urine samples were consecutively collected over 48 h. In analogy to di-iso-nonylphthalate (DINP) metabolism, we quantified the simple monoester mono-isononyl-cyclohexane-1,2-dicarboxylate (MINCH) and its secondary oxidized metabolites with HPLC–MS/MS via isotope dilution analysis. Additionally, we quantified the unspecific full breakdown product, cyclohexane-1,2-dicarboxylic acid (CHDA), via standard addition. All postulated metabolites were present in all samples analyzed. The unspecific CHDA was identified as the major urinary metabolite representing 23.7 % of the dose as the mean of the three volunteers (range 20.0–26.5 %). 14.8 % (11.3–16.7 %) of the dose was excreted as monoesters with oxidative modifications, in particular OH-MINCH 10.7 % (7.7–12.9 %), oxo-MINCH 2.0 % (1.5–2.6 %) and carboxy-MINCH 2.0 % (1.8–2.3 %). Less than 1 % was excreted as the simple monoester MINCH. In sum, 39.2 % (35.9–42.4 %) of the DINCH® dose was excreted as these metabolites in urine within 48 h. Over 90 % of the metabolites investigated were excreted within 24 h after application. The secondary oxidized metabolites, with elimination half-times between 10 and 18 h, proved to be apt and specific biomarkers to determine DINCH® exposure. With this study, we provide reliable urinary excretion factors to calculate DINCH® intakes based on these metabolites in environmental and occupational studies.
  Archive für Toxikologie 12/2012; · 4.67 Impact Factor
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  Available from: Jürgen Angerer

  Dataset: V-597

  Peter Welge, Rainer Bramer, Dietmar Breuer, Jens-Uwe Hahn, Beate Pesch, Anne Spickenheuer, Katrin Förster, Boleslaw Marczynski, Thomas Brüning, Jürgen Angerer, Monika Raulf-Heimsoth, Heiko U. Käfferlein, Thomas Mensing
• Article: Internal exposure to carcinogenic polycyclic aromatic hydrocarbons and DNA damage: a null result in brief.

  Heiko U Käfferlein, Boleslaw Marczynski, Patrice Simon, Jürgen Angerer, Hans-Peter Rihs, Michael Wilhelm, Kurt Straif, Beate Pesch, Thomas Brüning
  Archive für Toxikologie 06/2012; 86(8):1317-21. · 4.67 Impact Factor
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  Available from: Holger Martin Koch

  Article: Bisphenol A in 24 h urine and plasma samples of the German Environmental Specimen Bank from 1995 to 2009: A retrospective exposure evaluation.

  Holger M Koch, Marike Kolossa-Gehring, Christa Schröter-Kermani, Jürgen Angerer, Thomas Brüning
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  ABSTRACT: Human exposure to Bisphenol A (BPA) is omnipresent. Both the extent of the exposure and its toxicological relevance are controversially discussed. We aim to reliably determine and evaluate the extent of BPA body burden in the German population from 1995 to 2009 based on 600 24 h urine samples and corresponding plasma samples from the Environmental Specimen Bank. We determined total and unconjugated BPA in urine and plasma using on-line solid-phase extraction high-performance liquid chromatography coupled to isotope dilution tandem mass spectrometry with a limit of quantification (LOQ) of 0.1 μg/l. In the stored urines, total BPA was quantifiable in >96% (median: 1.49 μg/l; 95th percentile: 7.37 μg/l), whereas unconjugated BPA was quantifiable only in <15% of the samples. Total BPA concentrations decreased over time, but 24 h urine volumes increased. Therefore, daily intakes calculated from the 24 h urines remained rather constant at a median of 0.037 and a 95th percentile of 0.171 μg BPA/kg body weight/day. In 60 corresponding plasma samples, total BPA levels were generally below the LOQ of 0.1 μg/l and, if quantifiable, most BPA was unconjugated, thus hinting to external contamination. We see total BPA in urine as the most appropriate and robust marker for BPA exposure assessment (if controlled for BPA contamination). Unconjugated BPA in urine and unconjugated or total BPA in plasma where contamination or breakdown of the glucuronide cannot be ruled out are of no value for human exposure assessment.
  Journal of Exposure Science and Environmental Epidemiology 05/2012; 22(6):610-6. · 2.93 Impact Factor
• Article: Perfluorinated compounds (PFC) hit the headlines

  Peter H. Roos, Jürgen Angerer, Hermann Dieter, Michael Wilhelm, Detlef Wölfle, Jan G. Hengstler
  Archive für Toxikologie 04/2012; 82(1):57-59. · 4.67 Impact Factor