Publications (6) View all
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Article: Purification and partial characterization of an agglutinin from Octopus maya serum.
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ABSTRACT: A 66-kDa lectin (OmA) was purified from the serum of the Yucatan peninsula endemic octopus (Octopus maya) by a single step affinity chromatography on glutaraldehyde-fixed stroma from rat erythrocytes. OmA corresponds to 0.8% of the total circulating protein in the hemolymph; it is composed of three equal subunits of 22kDa each, and 7.4% of linked carbohydrates. The amino acids' composition indicated that agglutinin contained mainly aspartic and glutamic acids, and cysteine and methionine were identified in minor proportion. OmA agglutinates mainly rat, guinea pig, and rabbit erythrocytes, and this activity is partially inhibited by galactosamine, melobiose, galacturonic acid, mannose, and methyl alpha and beta galactosides. Hemagglutinating activity is not dependent on divalent cations, such as Ca(2+), Mg(2+), or Mn(2+). The OmA subunits showed no identity for any lectin in databases but partial identity with the type A hemocyanin from Octopus dolfleini hemolymph; the main similarities are related to tyrosinase domains and copper A and B sites that conform to the oxygen-binding site of hemocyanin.Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 05/2010; 156(1):1-5. · 1.61 Impact Factor -
Article: Review: Immunity mechanisms in crustaceans.
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ABSTRACT: Crustacean aquaculture represents a major industry in tropical developing countries. As a result of high culture densities and increasing extension of aquaculture farms, the presence of diseases has also increased, inducing economic losses. Invertebrates, which lack adaptive immune systems, have developed defense systems that respond against antigens on the surface of potential pathogens. The defense mechanisms of crustaceans depend completely on the innate immune system that is activated when pathogen-associated molecular patterns are recognized by soluble or by cell surface host proteins, such as lectins, antimicrobial, clotting, and pattern recognition proteins, which, in turn, activate cellular or humoral effector mechanisms to destroy invading pathogens. This work is aimed at presenting the main characteristics of the crustacean proteins that participate in immune defense by specific recognition of carbohydrate containing molecules, i.e. glycans, glycolipids, glycoproteins, peptidoglycans, or lipopolysaccharides from Gram-negative and Gram-positive bacteria, viruses, or fungi. We review some basic aspects of crustacean effector defense processes, like agglutination, encapsulation, phagocytosis, clottable proteins, and bactericidal activity, induced by these carbohydrate-driven recognition patterns.Innate Immunity 07/2009; 15(3):179-88. · 4.00 Impact Factor -
Article: The use of monoclonal antibodies anti-lectin from freshwater prawn Macrobrachium rosenbergii (DeMan, 1879) in the recognition of protein with lectin activity in Decapod's hemolymph.
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ABSTRACT: We determined the cross-reactivity of a monoclonal antibody against the Macrobrachium rosenbergii lectin with proteins in the hemolymph from Procambarus clarkii (Pc), Procambarus americanus (Pa), Litopenaeus setiferus (Ls), and Pseudothelphusa americana (Psa). Crustaceans' hemolymph agglutinated erythrocytes from rat, mouse, guinea pig, and rabbit. Decapods' hemolymph hemagglutinating activity was inhibited by N-acetylated carbohydrates as well as by antibodies. Western blot assays indicated that the antibodies recognized two main proteins of 97.5 and 80.9 kDa in all hemolymphs studied; moreover, ELISA assays indicated that, in PSa, 7.2% of total proteins showed crossreactivity with antibodies in Pa, Pc, and Lc hemolymphs represented 4.2, 3.1%, and 2.5%, respectively. Our results suggested that antibodies recognize the lectin active site in the crustacean species tested; we propose the use of antibodies as an immunological marker for lectin identification and quantification among crustaceans.Preparative Biochemistry & Biotechnology 02/2009; 39(3):308-22. · 0.47 Impact Factor -
Article: Purification and characterization of a lectin from the white shrimp Litopenaeus setiferus (Crustacea decapoda) hemolymph.
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ABSTRACT: A 291-kDa lectin (LsL) was purified from the hemolymph of the white shrimp Litopenaeus setiferus by affinity chromatography on glutaraldehyde-fixed stroma from rabbit erythrocytes. LsL is a heterotetramer of two 80-kDa and two 52-kDa subunits, with no covalently-liked carbohydrate, and mainly composed by aspartic and glutamic acids, glycine and alanine, with relatively lower methionine and cysteine contents. Edman degradation indicated that the NH2-terminal of the 80-kDa subunit is composed DASNAQKQHDVNFLL, whereas the NH2-terminal of the 52-kDa subunit is blocked. The peptide mass fingerprint of LsL was predicted from tryptic peptides from each subunit by MALDI-TOF, and revealed that each subunit showed 23 and 22%, respectively, homology with the hemocyanin precursor from Litopenaeus vannamei. Circular dichroism analysis revealed beta sheet and alpha helix contents of 52.7 and 6.1%, respectively. LsL agglutinate at higher titers guinea pig, murine, and rabbit erythrocytes its activity is divalent cation-dependent. N-acetylated sugars, such as GlcNAc, GalNAc, and NeuAc, were the most effective inhibitors of the LsL hemagglutinating activity. Sialylated O-glycosylated proteins, such as bovine submaxillary gland mucin, human IgA, and fetuin, showed stronger inhibitory activity than sialylated N-glycosylated proteins, such as human orosomucoid, IgG, transferrin, and lactoferrin. Desialylation of erythrocytes or inhibitory glycoproteins abolished their capacity to bind LsL, confirming the relevance of sialic acid in LsL-ligand interactions.Biochimica et Biophysica Acta 07/2005; 1724(1-2):86-93. · 4.66 Impact Factor -
Article: Lectina en L. Setiferus una alternativa en cultivo ante Enfermedades que Afectan al Cultivo de Camarones (Lectins from L. setiferus a possible solution for Culture Shrimp Diseases)
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ABSTRACT: El cultivo de camarones peneidos, es una actividad que genera ganancias del orden de billones de dólares en países como Ecuador, Brasil, China, y Tailandia. La mayor parte de la producción de estos países, se basan en el cultivo de Litopenaeus monodon. En América latina, Litopenaeus vannamei es la especie cultivada mas importante. Durante la rápida expansión de la camaronicultura, ha estado plagada por enfermedades, convirtiéndose en el problema más grande de la industria, causando perdidas millonarias en la industria; La mayoría de las enfermedades se hicieron evidentes en la última década del siglo pasado, en donde la mayoría de dichas enfermedades fueron causadas por virus que afectan a los camarones peneidos. El camarón blanco del Golfo de México aparenta ser una especie muy resistente a enfermedades virales, es por ello, que L. setiferus es considerada como una especie potencial para el desarrollo de esta actividad; estudios enfocados a conocer como funcionan los mecanismos de defensa en esta especie, son de vital importancia para el desarrollo planeado de la producción y el cultivo de la especie. La investigación del sistema inmune en la especie ha demostrado que es posible aumentar la resistencia a enfermedades, mediante la ingestión sustancias que modulen al sistema inmune y mediante programas que involucran prácticas básicas de buen manejo. El diagnostico de la enfermedad es difícil de realizar, si no se presentan síntomas o signos,. Para esto, se sugiere, que pueden usarse biomarcadores, mediante los cuales se puede monitorear el estado de salud de los organismos. Las lectinas son glicoproteínas de reconocimiento de carbohidratos, que se encuentran en la hemolinfa de crustáceos, considerando las características bioquímicas de las lectinas descubiertas, las hacen candidatas a ser usadas como marcardores de salud. Se pueden observar similitudes en cuanto a especificidad de lectinas encontradas a lo largo de crustáceos decápodos, lo que hace pensar que esta característica está conservada evolutivamente. La respuesta inmune en invertebrados está definida por la falta de memoria inmunológica y por la carencia de anticuerpos, sin embargo ha demostrado ser específica, ya que existen proteínas multifuncionales presentes en circulación, característica que hace a este sistema muy eficaz para responder ante la presencia de cualquier patógeno. The shrimp culture, it is an activity that generates earnings of the order of billions of dollars in countries as Ecuador, Brazil, China, and Thailand. Most of the production of these countries, is based on culture of only one or two species. In Latin America, Litopenaeus vannamei is the most cultivated species. During the rapid expansion of the shrimp culture, it has been infected by diseases, turning into the biggest problem of the industry, causing lost millionaires in the industry; The majority of the diseases became evident in the last decade of last century, where the majority of the above mentioned diseases were caused by virus that they affect the peneids shrimps. The white shrimp of the Gulf of Mexico shows off to be a species very resistant to viral diseases, that is the reason why L. setiferus is considered to be a potential species for the development of this activity [1] studies focused to knowing how the mechanisms of defense work in this species, perform vital importance for the development planned of the production and the culture of the species. The research of the immune system in the species has demonstrated that it is possible to increase the resistance to diseases, by means of the ingestion substances that they modulate to the immune system and by means of programs that involve basic practices of good managing. The diagnosis of the disease is difficult to realize, if they do not present symptoms or signs. For this, are suggested, that can be used biomarkers, by means of which the state of health of the organisms. The lectins are glycoproteins of recognition of carbohydrates, which they are find in the hemolymph of crustaceans, considering the biochemical characteristics of the disclosed lectins, do them candidates to being used like markers of health. Similarities can observe as for specificity of lectinas found along decapods crustaceans, which makes think that this characteristic is preserved by evolution. The immune response in invertebrates is defined by the lack of immunological memory and by the lack of antibodies, nevertheless it has demonstrated to be specific, since there exist multifunctional present proteins in traffic, characteristic that it does to this very effective system to answer before the presence of any pathogenic one.REDVET. 01/2005;
