Jose Atilio Aranguren Mendez

Dr, Genetica
Director de la Division de Investigacion
University of Zulia · Animal Production and Molecular Genetics.

Publications

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    Belkys Vasquez, José A. Aranguren-Méndez
    Logros y desafíos de la ganadería doble propósito 2014, Edited by Carlos González-Stagnaro, Ninoska Madrid-Bury, Eleazar Soto Belloso, 09/2014: chapter XX: pages 192-200; Fundación GIRARZ., ISBN: 978-980-6863-16-3
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    Rafael María Román Bravo, José Atilio Aranguren Méndez
    Logros & Desafíos de la Ganadería Doble Propósito, 2014 edited by Carlos González Stagnaro;Ninoska Madrid Bury y Eleazar Soto Belloso, 09/2014: chapter XV: pages 137-156; , ISBN: ISBN 978-980-6863-16-3
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    José Atilio Aranguren Méndez, Rafael María Román Bravo
    Logros & Desafíos de la Ganadería Doble Propósito, 2014 edited by Carlos González Stagnaro;Ninoska Madrid Bury y Eleazar Soto Belloso, 09/2014: chapter XIV: pages 120-136; , ISBN: ISBN 978-980-6863-16-3
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    VI Congreso Venezolano de Mejoramiento Genético y Biotecnología Agrícola, Guárico-Venezuela; 06/2014
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    ABSTRACT: This research was carried out in order to verify by simulation Mendel's laws and seek for the clarification, from the author's point of view, the Mendel-Fisher controversy. It was demonstrated from: the experimental procedure and the first two steps of the Hardy-Weinberg law, that the null hypothesis in such experiments is absolutely and undeniably true. Consequently, repeating hybridizing experiments as those showed by Mendel, it makes sense to expect a highly coincidence between the observed and the expected cell frequencies. By simulation, 30 random samples were generated with size equal to the number of observations reported by Mendel for his single trait trial, in this case, seed shape; assuming complete dominance, with genes A and a; likewise, it was simulated the results for the experiment with two traits, segregating in separate chromosomes, in this case seed shape, as before, and albumen color, with genes B and b, both loci with complete dominance. In the case of a single trait, the data only showed evidence for rejec-ting the null hypothesis (H o) in 1/30 samples, with (P<0.05). In the case of the 30 samples of the two traits experiment, (H o) was rejected only on 3/30 times, when it was set a = 0.05. In both simulations there was a high correspondence between the observed and expected cell frequencies, which is simply due to the fact that (H o) is true, and under these conditions, that is what would to expect. It was concluded, that Mendel had no reason to manipulate his data in order to make them to coincide with his beliefs. Therefore, in experiment with a single trait, and in experiments with two traits assuming complete dominance, segregation ratios are 3:1; and 9:3:3:1, respectively. Consequently, Mendel's laws, under the conditions as were described are absolutely valid and universal. RESUMEN El propósito de esta investigación fue verificar por simulación las leyes de Mendel y contribuir al esclarecimiento de la controversia Fisher-Mendel. Para ello, se demuestra a partir de: el procedimiento experimental y los dos primeros pasos de la ley de Hardy-Weinberg que la hipótesis nula, en tales experimentos es, sin lugar a dudas absolutamente cierta. En consecuencia, al repetir experimentos de híbridos tal como los planteó Mendel, lo más lógico es esperar una alta coincidencia en-tre las frecuencias observadas y las esperadas. Mediante simulación, se generaron 30 muestras aleatorias con tamaño igual al número de observaciones reportadas por Mendel para un sólo carácter, en este caso, la forma de la semilla, asumiendo dominancia completa, con alelos A y a. Así mismo, se simularon los resultados del experimento con dos caracteres, segregando en cromosomas independientes, en este caso la forma de la semilla y el color del albumen, este último con alelos B y b; asumiendo dominancia completa en ambos loci. Para el caso de un carácter, los resultados sólo muestran evidencia para rechazar la hipótesis nula (H o) en 1/30 muestras, con (P<0,05). En el caso del experimento con dos caracteres, (H o) fue rechazada en 3/30, oportunidades; cuando se estableció a=0,05. Se observó una alta correspondencia entre las frecuencias observadas y las esperadas, lo cual es debido al hecho de que la (H o) es verdadera, y en esas condiciones es lo que es de esperar. Se concluye que Mendel no tenía razón alguna, para manipular los resultados con el propósito de hacerlos coincidir con sus creencias. Por lo tanto en experimentos con uno y dos caracteres, y dominancia completa, las proporciones de segregación son 3:1 y 9: 3: 3: 1, respectivamente. En consecuencia, las leyes de Mendel, en las condiciones como las describe son absolutamente válidas y universales.
    Revista Cientifica, FCV-LUZ. 01/2014; XXIV(1):34-48.
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    Revista científica de veterinaria 01/2014; XXIV(5):428-435. · 0.14 Impact Factor
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    ABSTRACT: Genetic diversity in the Venezuelan Creole goat breed was estimated using PCR amplification of 29 microsatellites in fifty animals. The used sets were the ones proposed by the FAO and ISAG for biodiversity studies. The results of the present study showed that the genetic diversity was high, with an expected heterozygosity average of 0.65 and a mean number of alleles per locus of 6.2. Genetic diversity measures revealed a good status of biodiversity in the Venezuelan Creole goat breed. The systematic use of molecular markers will facilitate the comprehensive management of the populations and will constitute a good strategy to preserve this specie.
    Revista científica de veterinaria 05/2013; 23(3):238-244. · 0.14 Impact Factor
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    ABSTRACT: Tropical sheep breeds are characterized by being haired breeds, mainly used for meat production. The growth of lambs during the pre-weanning first phase depends almost exclusively on mother's milk production, hence the attention given to milk proteins and their genotype. Beta-lactoglobulin plays a reponderant role within these proteins as it is the most abundant milk protein in ruminants, and it is known that in sheep it is found in chromosome 3 and has polymorphic variants. For this study, an experiment was carried out with 20 tropical West African sheep to determine the genotype of the BLG and its effect on milk production. Genotyping was performed by PCR-RFLP technique and the milk production estimate by lambs’s double weighing. The results showed BLG allele frequencies of 0.80 for the homozygous AA, 0.20 for the heterozygote AB and absence (0.00) for BB homozygotes, no showed effect on milk production during breastfeeding. It is noteworthy that despite the small number of individuals analyzed, a predominance of genotype AA, which has been associated with a greater amount of casein in milk and therefore cheese yield is shown. These findings are useful for establishing patterns of marker-assisted selection, and thus giving these species a greater scientific interest in production systems.
    Actas Iberoamericana de Producción Animal. 10/2012; 2(2):193-196.
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    1er Congreso Venezolano de Ciencia, Tecnologia e Innovación. LOCTI - PEII, Caracas; 10/2012
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    ABSTRACT: The Human Leukocyte Antigen (HLA) is coded by a group of genes that are located on chromosome 6 region p21.3. In order to know the genetic structure of the population of San José de Heras, Zulia State, through markers HLA class I (HLA-A,-B,-C) and class II (HLA-DRB1, -DQB1), we selected 40 individuals, whose DNA were processed through the Polymerase Chain Reaction (PCR) and then proceed to conventional hybridization using Reverse Dot Blot technique (SSO). The allelic distribution of the HLA loci studied were in Hardy-Weinberg equilibrium and higher allelic frequencies for the various HLA loci were for class I, HLA-A*02 (0.25), B*57 (0.21) and C*04 (0.22). For class II, HLA-DRB1*15 (0.22) and DQB1*06 (0.31). Data generated in this study are consistent with those reported in the literature worldwide for negroid populations. The study of the genetic variability of different ethnic groups in our country, would identify diagnostic markers and / or prognosis of diseases, and establish correspondence between organ donor-recipient to prevent rejection of transplanted tissue or to minimize the use of drugs immunosuppressive.
    Ciencia. 01/2012; 20(2):98-108.
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    ABSTRACT: La edad de incorporación de las hembras bovinas a la etapa reproductiva es una característica de importancia económica en los rebaños de Doble Propósito (DP), ya que contribuye a reducir el intervalo generacional. Con la finalidad de determinar la edad al primer servicio(E1S), la edad al primer parto (E1P) y los factores genéticos y no genéticos que las afectan, se evaluaron 10928 registros pertenecientes a 5 fincas asociadas a la Fundación Ganadoble. Fueron considerados los efectos fijos finca (F = 1,..., 5), año (A = 1987,..., 2004), época de nacimiento (E = Seca, Lluvia), la interacción A*E y el componente genético que se clasificó según la proporción de genes taurus e índicus (PG =desde 7/8t:1/8i hasta 8/8i). Los resultados indican que con excepción de la E, todos los efectos influyeron significativamente sobre E1S y E1P (P<0,01), siendo 27,2 y 37,5 meses los promedios obtenidos para esas características. En cuanto al componente genético los animales mestizos 4/8t:4/8i y 5/8t3/8i presentaron los valores más bajos para E1S con 31 y 31,25 meses y para E1P de 40,6 y 40,8 meses, respectivamente. Los valores obtenidos resultaron ser superiores a las recomendaciones citadas en la literatura para rebaños DP, por lo que la disminución de estos valores como indicadores de eficiencia en el reemplazo de las hembras de la población, debe ser tomada en cuenta en los programas de manejo y mejora genética de los rebaños mestizos DP.
    Revista científica FCV-LUZ. 01/2012; 22(Supl. 1):484.
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    Paola Torres.-Rodríguez, José Aranguren-Méndez
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    ABSTRACT: Se plantea el uso de los cruzamientos entre especie de los equinos, para la formacion de hibridos en utlidad funcional para fines productivos. Es una revision bibliografica en donde se estudia el uso de los hibridos equinos en la produccion animal
    Reproducción Equina, 1 edited by Jorge Luis Rubio Guillen, 11/2011: chapter Manejo Reproductivo para Obetener Híbridos Equinos: pages 149; Astro Data., ISBN: 978-980-6863-12-5
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    Jose Aranguren-Mendez
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    ABSTRACT: La mastitis es una enfermedad compleja, la cual ha sido definida como la inflamación de la glándula mamaria, como consecuencia de la introducción y multiplicación de microorganismos patógenos en el interior de la glándula, causados generalmente por gérmenes bacterianos tales como: Staphylococcus spp., Streptococcus spp., Pseudomonas spp., Mycoplasma spp., y algunos coliformes tales como Escherichia coli (Belloda & Ponce, 2008). La mastitis resulta ser la enfermedad más frecuente y costosa que afecta al ganado lechero y se reporta como la principal causa de descarte de vacas lecheras. Cerca de 2 billones de dólares se gastan anualmente debido a esta enfermedad en EE.UU. o en Europa, lo que corresponde aproximadamente al 10% del valor total en ventas de leche (Pérez-Cabal et al., 2009). Las glándulas mamarias son glándulas dérmicas modificadas, que se clasifican como exocrinas, cuya función es secretar leche para la alimentación de los animales jóvenes, durante períodos diversos de vida post-natal; crecen durante la preñez y comienza a secretar leche después del parto (Rainard & Riollet, 2006).
    Manejo de la Mastitis Bovina y Programas de Control, 1 edited by Julio Boscan, 10/2011: chapter Seleccion Genetica Para Resistencia a la Mastitis: pages 188; Astro Data., ISBN: 978-980-6863-11-8
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    ABSTRACT: DNA samples from 163 animals of Limonero cattle breed were analyzed (44 males and 119 females) at several candidates genes for milk and meat production (CSN3, BLG, CAST and CAPN) in,. Genotyping was performed by PCR-RFLP technique, The allele, genotype frequencies and EHW were compared using chi-square. The results showed the CSN3 allele frequency of 0.11 for the homozygous AA, 0.56 for the heterozygous AB and 0.33 for the BB homozygote, the BLG frequencies were 0.07, 0.29 and 0.64, for genotypes AA, AB and BB, respectively. The data obtained for meat quality genes were 0.84, 0.03 and 0.13 for genotypes AA, AB and BB, respectively, for the CAST gene. For the CAPN gene the results were AA (0, 1 5), AG (0.56) and GG (0.29). It is noteworthy that the finding of a high frequency of allele B for milk proteins provides useful evidence for the production of cheeses. Besides the presence of genotypes GG for the CAPN gene and AA for the CAST gene, could give a great potencial for meat tenderness and quality selection. These results will be very useful to establish marker-assisted selection plans, and will give an added value to the Creole Limonero cattle in production systems.
    Actas Iberoamericana de Producción Animal. 09/2011; 1(1):199-202.
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    ABSTRACT: Libido and serving capacity score of 12 «Criollo Limonero» bulls (49.2±4.2) and 30.6±3.8 m and 531.0 ±9.5 and 329.0 ± 4.3 kg BW) were determined. Test were carried out on 10 tests (one/week), by using restrained female with estrus induced (5 test) or not in estrus (5 test). Bulls stimulated by non-estrus female differed (P<0.05) in reaction time. Eight bulls classified as high libido/serving capacity score (HL) and 4 as having low (LL). The HL bulls produced more ejaculates (3.88±0.13 vs 1.59±0.17; P<0.001) and the reaction time (0.48±0.14) vs 1.55±0.19 min; P<0.01) and latency period were shorter (1.04±0.12 vs 2.06±0.18 min; P<0.01) than LL bulls. Results suggested that estrogenized females are not necessary for sexual behaviour evaluation of "Criollo Limonero" bulls.
    Revista de la Facultad de Agronomia 09/2011; 28(1):505-513. · 0.13 Impact Factor
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    MARIA PORTILLO, JOSE ARANGUREN-MENDEZ
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    ABSTRACT: Durante los últimos años la Genética Animal ha tomado un gran auge, debido a la obtención de genotipos mejorados, adaptados a medios ambientes específicos del trópico o subtrópico americano (Aranguren-Méndez et al., 2007); así mismo, en virtud del vertiginoso avance de la genética molecular, producto del estudio sobre el genoma humano y mas reciente por los trabajos específicos con el genoma bovino (Elsik et al., 2009), señalando su beneficio para la ciencia animal (Fríes&Ruvinsky, 1999). A ello ha contribuido la dramatica reducción de los costos por genotipado animal que se ha venido observando en los últimos años (Matukumalli et al., 2009).
    INNOVACION & TECNOLOGIA EN LA GANADERIA DE DOBLE PROPOSITO, 1 edited by CARLOS GONZALEZ-STAGNARO, NINOSKA MADRID BURY, ELEAZAR SOTO BELLOSO, 05/2011: chapter CAPITULO XXVI: pages 944; ASTRO DATA., ISBN: 978-980-6863-10-1
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    ABSTRACT: Haplotype data were obtained from sample of 292 male from different regions in Zulia state, in the northwest of Venezuela, for 11 Y-chromosome STR’s (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438 and DYS439). Genetic Distances between these regions and others published were estimated. The highest haplotype diversity was observed in the group of Maracaibo (0.9969), whereas Yukpas showed the lowest levels (0.3182). 207 haplotypes were observed, of which 192 were found to be unique. The degree of mixing showed a high European component In Maracaibo and Toas Island (86.25% and 90.32%, respectively). Wayúu group showed Amerindian contribution (76.52%). The San Jose de Heras group showed African component, lower than expected, 54.93%, followed by a European contribution (42.27%). Indigenous groups, Bari and Yukpa, showed only Amerindian component. The results show the usefulness of these polimorfismos as markers of different ethnic groups that reside inside the Zulia state and remark the importance of using them as local databases in forensic genetics.
    CIENCIA. 04/2011; 19(2):99-116.
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    J. Jordana, A. Ferrando, J. Marmi, R. Avellanet, J.A. Aranguren-Méndez, F. Goyache
    Livestock Science 01/2011; · 1.25 Impact Factor
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    ABSTRACT: In order to assess polymorphism of the calpastatin (CAST) gene in Limonero Creole cattle, blood samples were collected from 157 animals (44 male and 113 female). Genetic characterization was carried out by Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) using the XmnI restriction enzyme. The allelic and genotypic frequencies were compared using chi-square tests. Results showed frequencies of 0.84, 0.03 and 0.13 for genotypes AA, AB and BB, respectively. Allelic frequencies were 0.85 and 0.15 for alleles A and B, respectively. Chi-square tests showed that the population was not in Hardy-Weinberg equilibrium. It is important to mention that finding the high frequency of the CAST gene A allele and its correlation with meat tenderness and quality, could be useful for establishing selection plans assisted by markers, in order to increase beef production and quality and, thereby, give the Limonero Creole breed a potential advantage
    Revista de la Universidad del Zulia. 01/2011; 2(2):194-202.
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    Revista científica de veterinaria 01/2011; XXI:523. · 0.14 Impact Factor

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