Jörg Lehmann

Publications (37) View all

• Source

  Available from: Ulf Wagner

  Article: Identification and evaluation of novel synovial tissue biomarkers in rheumatoid arthritis by laser scanning cytometry.

  Christiane Fueldner, Anja Mittag, Jens Knauer, Maria Biskop, Pierre Hepp, Roger Scholz, Ulf Wagner, Ulrich Sack, Frank Emmrich, Attila Tárnok, Joerg Lehmann
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  ABSTRACT: Suitable biomarkers are essential for therapeutic strategies in personalized medicine in terms of diagnosis as well as of prognosis. With highly specific biomarkers, it is possible, for example, to identify patients with poor prognosis, which enables early intervention and intensive treatment. The aim of this study was to identify and validate biomarkers and possible combinations for a prospective use in immunoscintigraphy, which may improve diagnosis of rheumatoid arthritis (RA) patients with consideration of inflammatory activity in the affected joints. Therefore, we tested several monoclonal antibodies (mAbs) directed against cellular-surface molecules on cells likely to be involved in the pathogenesis of RA. Synovial tissue from patients with long-standing RA (accompanied by synovitis with varying states of current activity) and patients with acute non-RA arthritis were stained for surface molecules on different cell types by using fluorochrome-labeled antibodies. Tissue analysis was done by laser scanning cytometry (LSC), and statistical evaluation, by discriminant analysis and ROC analysis. CD11b, HLA-DR, CD90, and CD64 revealed significant differences between tissues from patients with RA and acute non-RA arthritis. Especially with the expression of CD64, both patient cohorts could be discriminated with high sensitivity and specificity. RA classification was improved by simultaneously investigating the expression of two or three different surface proteins, such as HLA-DR, CD90, and CD29 in the tissue. The simultaneous analysis of CD64 together with CD304 or the combination of CD11b and CD38 was suitable for the identification of RA patients with high current activity in synovitis. In this study, we showed that LSC is a novel reliable method in biomarker prevalidation in RA. Hence, identified mAbs in situ may allow their potential use in in vivo approaches. Moreover, we proved that biomarker-combination analysis resulted in better discrimination than did single-marker analysis. Combinations of these markers make a novel and reliable panel for the discrimination between RA and acute non-RA arthritis. In addition, further expedient combinations may be novel promising biomarker panels to identify current activity in synovitis in RA.
  Arthritis research & therapy 01/2012; 14(1):R8. · 4.27 Impact Factor
• Source

  Available from: Ulrich Sack

  Article: Induction of a rapid progessive cartilage destruction in SCID mice by intraarticular application of a murine fibroblast like cell line

  U Sack, A Hirth, B Funke, K Wiedemeyer, S Konrad, J Lehmann, F Emmrich
  Arthritis Research & Therapy 04/2012; 3:1-1. · 4.45 Impact Factor
• Source

  Available from: Benjamin Funke

  Article: Reduction of cartilage destruction in a rapid progressive arthritis model in SCID mice

  U Sack, A Hirth, B Funke, K Wiedeneyer, F Kahlenberg, U Anderegg, F Krahnert, J Lehmann, F Emmrich
  Arthritis Research & Therapy 04/2012; 4:1-1. · 4.45 Impact Factor
• Source

  Available from: Jörg Lehmann

  Article: Evaluation of the preventive capacities of a topically applied azithromycin formulation against Lyme borreliosis in a murine model.

  Jens Knauer, Inke Krupka, Christiane Fueldner, Joerg Lehmann, Reinhard K Straubinger
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  ABSTRACT: Systemic antibiotic treatment of Lyme borreliosis is effective during the early stages of the infection, while chronic manifestations of the disease may remain refractory and difficult to treat. This study was carried out in order to evaluate the potential of topically applied azithromycin to eliminate the spirochaetal organisms in the skin of the freshly bitten host and thereby prevent Lyme borreliosis. Laboratory mice were challenged with Borrelia burgdorferi sensu stricto by needle inoculation or via infected ticks as vectors. Then, an azithromycin-containing formulation was applied once daily to the sites of exposure for three consecutive days. In the case of needle inoculation, a 5% azithromycin formulation was applied starting 1 h, 3 days and 5 days after infection. In the case of tick exposure, 4%, 10% and 20% azithromycin formulations were applied, starting directly after the detachment of the engorged ticks. Subsequently, the infection status of the mice was determined. Concentrations of azithromycin in murine skin were >3800-fold higher than the published minimal inhibitory concentration for B. burgdorferi as soon as 3 h after the first application. After needle inoculation, spirochaetes were not detectable in all infected mice after treatment, if the first application started 1 h or even after 3 days post-infection. Furthermore, no borrelial organisms were detected after topical treatment when ticks were used for spirochaete inoculation. Our data indicate that topical treatment with a formulation containing azithromycin is a promising approach to prevent Lyme borreliosis shortly after a tick bite.
  Journal of Antimicrobial Chemotherapy 09/2011; 66(12):2814-22. · 5.07 Impact Factor
• Article: Thy-1 (CD90) regulates the extravasation of leukocytes during inflammation.

  Kathleen Schubert, Tobias Polte, Ulrike Bönisch, Sina Schader, Rafaela Holtappels, Guido Hildebrandt, Jörg Lehmann, Jan C Simon, Ulf Anderegg, Anja Saalbach
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  ABSTRACT: Human Thy-1 (CD90) has been shown to mediate adhesion of inflammatory cells to activated microvascular endothelial cells via interaction with Mac-1 (CD11b/CD18) in vitro. Since there are no data showing the physiological relevance of Thy-1 for the recruitment of inflammatory cells in vivo, different inflammation models were investigated in Thy-1-deficient mice and littermate controls. In thioglycollate-induced peritonitis, the number of neutrophils and monocytes was significantly diminished in Thy-1-deficient mice. During acute lung inflammation, the extravasation of eosinophils and monocytes into the lung was significantly reduced in Thy-1-deficient mice. Moreover, during chronic lung inflammation, the influx of eosinophils and monocytes was also strongly decreased. These effects were independent of Thy-1 expression on T cells, as shown by the transplantation of WT BM into the Thy-1-deficient mice. In spite of the strong Thy-1 expression on T cells in the chimeric mice, the extravasation of the inflammatory cells in these mice was significantly diminished, compared to control mice. Finally, the altered number and composition of infiltrating leukocytes in Thy-1-deficient mice modified the chemokine/cytokine and protease expression at the site of inflammation. In conclusion, Thy-1 is involved in the control of inflammatory cell recruitment and, thus, also in conditioning the inflammatory microenvironment.
  European Journal of Immunology 03/2011; 41(3):645-56. · 5.10 Impact Factor