Publications (39) View all
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Article: Effect of conjugated linoleic acid supplementation on weight loss and body fat composition in a Chinese population.
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ABSTRACT: Conjugated linoleic acid (CLA) has several benefits, including body fat reduction, as proved in animals. However, the results of CLA-induced body composition alterations in humans are inconsistent, and no related data are available for Chinese populations. This study aimed to determine whether CLA affects body weight (BW) loss and body composition of overweight and obese Chinese subjects. In this randomized, double-blind, placebo-controlled trial, subjects with a body mass index (BMI) of 24 to 35 kg/m(2) randomly received 1.7 g of cis-9,trans-11 and trans-10,cis-12 CLA (n = 30) or placebo (salad oil; n = 33) in 200 mL of sterilized milk twice daily for 12 wk. Changes in body composition were determined by bioimpedance measurements. Sixty-three subjects completed the study (CLA, n = 30). After 12 wk, compared with the baseline, the BW, BMI, total fat mass, fat percentage, subcutaneous fat mass, and waist-to-hip ratio decreased in the CLA group (P < 0.05). The CLA group was stratified by BMI and gender. The BW, BMI, subcutaneous fat mass, and waist-to-hip ratio decreased in 27 subjects with a BMI ≥ 27, and these indices, except subcutaneous fat mass, were lower in female subjects. The levels of total cholesterol, triacylglycerol, low-density lipoprotein, and plasma fasting glucose increased, whereas those of high-density lipoprotein decreased after 3 mo of CLA treatment. The changes were not significantly different from the baseline values. The supplementation of CLA for 12 wk in overweight and grade I obese Chinese subjects yielded lower obesity indices, with no obvious adverse effects.Nutrition 01/2012; 28(5):559-65. · 3.03 Impact Factor -
Article: A novel technique for liver retraction in laparoscopic bariatric surgery.
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ABSTRACT: During bariatric surgery in morbidly obese patients, the surgeon's operative view is often obscured by the hypertrophic fatty left lobe of the liver. The use of a conventional liver retractor mandates an additional subxiphoid wound, resulting in pain and scar formation, in addition to the risk of iatrogenic liver injury during retractor insertion. To overcome these limitations, we developed a simple, rapid, and safe technique for liver retraction--V-shaped liver suspension technique (V-LIST)--by using a Penrose drain and laparoscopic stapler. A silicone Penrose drain was inserted into the peritoneal cavity and stapled to the pars condensa of the lesser omentum and parietal peritoneum using a laparoscopic stapler. The left lobe of the liver was retracted by the V-shaped suspension. At the end of the surgery, the drain could be easily removed. In October 2009-February 2010, 14 patients underwent liver retraction with the use of this technique. We performed 12 Roux-en-Y gastric bypasses and 2 sleeve gastrectomies. This series also included three cases of single incision transumbilical laparoscopic surgery. The mean time required to complete the liver retraction was 8 min 21 s (range, 2-18 min 40 s). Retraction was appropriate in all patients, without the need for additional retractors or conversion. There were no V-LIST-related perioperative complications. Our V-LIST technique using a Penrose drain is safe and simple. It has potential applications in single incision laparoscopic bariatric procedures.Obesity Surgery 05/2011; 21(5):676-9. · 3.29 Impact Factor -
Article: Laparoscopic Roux-en-Y gastric bypass for the treatment of type II diabetes mellitus in Chinese patients with body mass index of 25-35.
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ABSTRACT: Laparoscopic Roux-en-Y gastric bypass (LRYGB) can dramatically ameliorate type 2 diabetes mellitus (T2DM) in morbidly obese patients. However, there is little evidence supporting the effectiveness of LRYGB in low body mass index (BMI) patients. The study was designed to evaluate the safety and results of LRYGB for achieving T2DM remission in patients with BMI in the range of 25-35 kg/m(2). Twenty-two patients (two men and 20 women) with T2DM underwent LRYGB. Data on patient demographics, BMI, co-morbidities, and details of diabetes mellitus, including disease duration, family history, medication use, and remission, were prospectively collected and analyzed. The mean age was 47 years (range, 28-63 years), mean BMI was 30.81 (range, 25.00-34.80 kg/m(2)), and mean duration of T2DM onset was 6.57 years (range, 1-20 years). Sixteen (72.27%) patients had a family history of T2DM. There was no mortality, but two (9%) patients experienced complications: an early gastrojejunostomy hemorrhage and frequent loose stools that required revision surgery. At 12 months, 14 (63.6%) patients showed T2DM remission, six (27.3%) showed glycemic control, and two (9.1%) showed improvement. The group achieving remission had a higher BMI (p = 0.001), younger age (p = 0.002), and shorter duration of diabetes (p = 0.001). These three factors may be predictors of diabetes resolution at 12 months. Early intervention in low-BMI patients yields better remission rates because age, BMI, and duration of T2DM predict glycemic outcomes.Obesity Surgery 04/2011; 21(9):1344-9. · 3.29 Impact Factor -
Article: Typhonium blumei extract inhibits proliferation of human lung adenocarcinoma A549 cells via induction of cell cycle arrest and apoptosis.
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ABSTRACT: Typhonium blumei Nicolson & Sivadasan is a traditional Chinese medicinal herb endowing with detumescence, detoxification, anti-inflammation activities, and has been used as a folk prescription on anticancer in Taiwan. The purpose of this study is to investigate the inhibitory effect of Typhonium blumei (Tb) extract on the viability of different cancer cells and the apoptotic effect of this extract on A549 lung cancer cells. Human A549 cell line and other cancer cell lines were treated with different concentrations of Tb extract at different time intervals. Growth inhibition was determined by MTT assay. Apoptosis was detected by cell morphologic observation, cell cycle analysis, and immunoblot analysis on the expression of protein associated with cell death. GC-MS were used to determine the chemical constituents of this extract. The Tb extract had cytotoxicity toward A549 lung cancer cells (IC(50)=97.7 μg/ml), LNCaP prostate cancer cells (IC(50)=124.5 μg/ml) and MCF-7 breast cancer cells (IC(50)=125.8 μg/ml). Conversely, the adverse effects of Tb extract on normal embryonic lung fibroblast MRC-5 cells (IC(50)=245.5 μg/ml) and embryonic kidney fibroblast HEK293 cells (IC(50)=251.1 μg/ml) were comparatively low. Cytometric analysis results demonstrate that A549 cells were arrested at the G2/M phase by treatment with Tb extract. The extract induced A549 cell apoptosis via the mitochondrial pathway by down-regulating Bcl-2 and Bcl-xL protein expression, up-regulating Bax, Bad and Bak protein expression, and activating caspase-9 and caspase-3. Experimental results of bioactive compound analysis indicate that dibutyl phthalate, α-linolenic acid, phytol, campesterol, stigmasterol and β-sitosterol were the major bioactive ingredients of Tb extract. Although all these compounds had good anti-proliferative effects on A549 cells, campesterol (IC(50)=2.2 μM for 24h treatment) and β-sitosterol (IC(50)=1.9 μM for 24h treatment) displayed the greatest inhibitory activity. Experimental results of this study suggest that the Tb extract exerts potential anticancer activity through the growth inhibition and the apoptosis on A549 cells.Journal of ethnopharmacology 04/2011; 135(2):492-500. · 2.32 Impact Factor -
Article: Preliminary surgical results of single-incision transumbilical laparoscopic bariatric surgery.
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ABSTRACT: Recently, single-incision laparoscopic surgery (SILS) has been used for bariatric procedures, and this surgery is considered a type of minimally invasive surgery. When SILS is performed via the transumbilical route, the resultant abdominal wound is hidden and the cosmetic outcome is better. However, because of the small angle of manipulation and difficulty in liver traction, this technique is not used to perform complex bariatric surgery. In this prospective study, we used our novel technique, which involves the use of a liver-suspension tape and umbilicoplasty of an omega-shaped incision (omega umbilicoplasty), to perform laparoscopic bariatric surgery via the single-incision transumbilical (SITU) approach. We then assessed the safety and effectiveness of our surgical technique. We started performing and developing this technique from December 2008. Until July 2009, 40 consecutive patients underwent 40 bariatric procedures: two adjustable gastric band placements, six sleeve gastrectomies, and 32 Roux-en-Y gastric bypass operations, including five cases where concomitant cholecystectomy was performed. The mean operation time was 93.4 min and the mean duration of postoperative hospitalization was 1.15 days. No perioperative or postoperative complications or deaths occurred. Most patients were very satisfied with the cosmetic outcomes. Our technique can be safely and effectively used for SITU laparoscopic bariatric surgery. This technique will soon be used for advanced abdominal surgeries besides bariatric ones.Obesity Surgery 03/2011; 21(3):391-6. · 3.29 Impact Factor
