Jeffrey Kiplinger

Publications (2) View all

• Article: Evaluation of a new preparative supercritical fluid chromatography system for compound library purification: the TharSFC SFC-MS Prep-100 system.

  Katalin Ebinger, Harold N Weller, Jeffrey Kiplinger, Paul Lefebvre
  [show abstract] [hide abstract]
  ABSTRACT: Preparative HPLC-MS is often the method of choice for purification of small amounts (<100mg) of diverse new molecules, such as compound libraries for drug discovery. The method is robust, well proven, and widely applicable. In contrast, preparative supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) has seen only slow acceptance for the same application--despite some potential scientific and economic advantages. One of the reasons for slow adoption of SFC-MS is the lack of well-proven, robust, and commercially available instrumentation. In early 2009, TharSFC (a Waters Company, Pittsburgh, PA) introduced a new fully integrated system for preparative SFC-MS: The SFC-MS Prep-100. We report herein an objective evaluation of the SFC-MS Prep-100, including tests for pump and autosampler performance, sample recovery, sample carryover, fraction triggering, detector/fraction collector synchronization, and overall robustness. Our results suggest that the SFC-MS Prep-100 represents a significant advance over previous generation instrumentation.
  Journal of the Association for Laboratory Automation 06/2011; 16(3):241-9. · 1.42 Impact Factor
• Article: Orthogonality of SFC versus HPLC for small molecule library separation.

  Harold N Weller, Katalin Ebinger, William Bullock, Kurt J Edinger, Mark A Hermsmeier, Steven L Hoffman, David S Nirschl, Thomas Swann, Jiang Zhao, Jeffrey Kiplinger, Paul Lefebvre
  [show abstract] [hide abstract]
  ABSTRACT: Preparative HPLC and HPLC-MS are well established as the methods of choice for purification of pharmaceutical library compounds. Recent advances in supercritical fluid chromatography (SFC) have now made SFC a viable alternative to HPLC for this application. One of the potential arguments for using SFC in place of, or in addition to, HPLC is that it may offer different selectivity and thus has the potential for improved separation success rates. In this paper, we examine relative success rates for SFC and HPLC in obtaining adequate selectivity for successful separation. Our results suggest that use of SFC in addition to HPLC may result in a slight (1-2%) improvement in success rate compared to use of HPLC alone.
  Journal of Combinatorial Chemistry 10/2010; 12(6):877-82. · 3.41 Impact Factor