Publications (9) View all
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Article: Effects of orally administered antioxidants on micronuclei and sister chromatid exchange frequency in workers professionally exposed to antineoplastic agents.
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ABSTRACT: The widespread use of antineoplastic drugs in cancer treatment increased concern about possible hazard to workers involved in the preparation and administration of these drugs. In the present study, the effects of commercial antioxidative drug Oligogal Se on genome protection were analyzed in 15 nurses handling the antineoplastic drugs at the Oncology Department in comparison to twenty healthy volunteers. The nurses took antioxidant mixture Oligogal Se, consisting of vitamins C, E, A and selenium, one capsule per day, over a period of 6 months. Genome damage was measured in peripheral blood lymphocytes by usage of sister chromatid exchange test and the cytokinesis-block micronuclei test. The frequency of sister chromatid exchange (SCE) and micronuclei (MN) in the exposed group was significantly higher when compared to the control group (SCE, p<0.05; MN, p<0.01 respectively). After antioxidant supplementation, the frequency of sister chromatid exchange and micronuclei decreased (p<0.05) when compared with the values from the beginning of the study, but were still above the values of the control group. The effects of confounding factors such as cigarette smoking and cytostatics exposure time were also evaluated. The data indicated that Oligogal Se contributed to the decreasing of genome damages in workers handling the cytostatics.Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 04/2012; 50(8):2937-44. · 2.99 Impact Factor -
Article: Bioactivity of Lemon Balm Kombucha
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ABSTRACT: There is inadequate published data referring to bioactivity of lemon balm tea and its Kombucha. The aim of this study, therefore, was to investigate antimicrobial, antiproliferative, genotoxic, and antigenotoxic potential of lemon balm tea and its Kombucha with consuming acidity. Antimicrobial activity was determined by agar-well diffusion method. Cell growth effects were determined in HeLa, MCF7, and HT-29 human tumor cell lines. Genotoxic and antigenotoxic effects were determined using chromosome aberration assay in Chinese hamster cell line CHO-K1. Differences between control and treated groups were evaluated using analysis of variance, at significance level of p < 0.05. Kombucha from lemon balm tea (Melissa officinalis L.) exibited antimicrobial activity against prokaryotic microorganisms independently of their cell wall structure (both Gram-positive and Gram-negative bacteria), while there was no observed activity against eukaryots (yeasts and moulds). There was absence of genotoxic effects while antigenotoxic effects of lemon balm Kombucha and tea were confirmed on MMC-damaged CHO-K1 cells. For the explanation of cell growth effects that were not concentration dependent, concept of hormesis was used. Antiproliferative activity was lower compared with traditional Kombucha and Satureja montana L. Kombucha, with lemon balm tea showing higher activity than its Kombucha. KeywordsAntimicrobial activity-Cell growth activity-Chromosome aberration assay-Kombucha- Melissa officinalis L.-TeaFood and Bioprocess Technology 05/2012; · 3.70 Impact Factor -
Article: Effects of fullerenol nano particles C60(OH)24 on micronuclei and chromosomal aberrations frequency in peripheral blood lymphocytes
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ABSTRACT: Fullerenol C60(OH)24 nano particles (FNP) show various biological activities in different experimental models. We evaluated genotoxic and antigenotoxic effects of FNP on human peripheral blood lymphocytes (PBL) using two cytogenetic assays. The effects of FNP were evaluated on the frequency of chromosomal aberrations (CA) and micronuclei (MN) on undamaged and mitomycin C (MMC)-damaged PBL. FNP number distribution in a culture medium with serum showed that predominant particles were about 180 nm and 90 nm respectively. Cytogenetic assays showed that FNP decreased chromosomal aberrations and micronucleus frequency on the undamaged and the MMC-damaged human PBL at concentration range from 5.54 μM to 221.60 μM. Our research confirmed that FNP did not exhibit genotoxic but induced antigenotoxic effects at subcytotoxic concentrations on human lymphocytes.Digest Journal of Nanomaterials and Biostructures 04/2012; 7(2):673-686. · 1.20 Impact Factor -
Article: The activity of superoxide-dismutase in animal cell culture CHO-K1 after treatment with fullerenol and mytomicine C
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ABSTRACT: Eukaryotic cell survives in predominantly reduced conditions. Homeostasis of cellular redox system is an imperative of cell surviving and its normal metabolism. ROS are well recognized for playing a dual role as both deleterious and beneficial species, since they can be either harmful or beneficial to living systems. These species are mutagenic compounds known to lead to DNA damage, favor cell transformation, and contribute to the development of a variety of malignant diseases. All the effects of oxidants are influenced by the cellular antioxidant defenses. This multilayer system consists of low molecular weight components and several antioxidant enzymes. Superoxide dismutases (SODs) are the only enzymes dismuting superoxide radicals. Mitomycin C, a cross-linking agent, demonstrated genotoxicity in all in vitro and in vivo test systems in mammalian cells and animals. Water-soluble fullerenes are well known as cytotoxic agents for many cell lines in vitro. At the other side, fullerenols are good free radical scavengers and antioxidants both in vitro and in vivo. This paper investigates the effects of fullerenol on survival and fullerenol/ /mytomicine (MMC) treatment on superoxide-dismutase (SOD) activity in CHO-K1 cells. Samples were treated 3 and 24 h with fullerenol (C60(OH)24) at concentration range 0.01-0.5 mg/mL and survival was monitored with dye exclusion test (DET). The activity of total SOD was estimated in samples treated with chosen concentrations of fullerenol and MMC (0.5 and 0.1 mg/mL) after 3 and 24 h of cell incubation. Increasing of C60(OH)24 concentration leads to decreasing of percent of surviving cells 3 and 24 h after incubation. The activity of total SOD enhanced with higher concentration of fullerenol, while decreased in the highest concentration at both experimental points. In samples treated with MMC, as well as in samples treated with fullerenol (0.0625 mg/mL) + MMC was noticed boost in total SOD activity in comparison with controls. Treatment with fullerenol decreased SOD activity in rest of samples treated with MMC. Decreased activity of superoxide-dismutase in almost all samples treated with fullerenol and MMC might be contributed to antioxidative properties of fullerenol. Increased enzyme level at concentration of 0.0625 mg/mL may be due to its prooxidative activity.Hemijska Industrija. 01/2009; -
Article: Activity of lactate dehydrogenase and superoxide dismutase in the circulation of patients with breast carcinoma
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ABSTRACT: Background: A rapid increase in the turnover of malignant cells modulates the enzymes level in circulation and may be a prognostic sign of disease progression. We evaluated the activity of serum lactate dehydrogenase and superoxide dismutase in erythrocytes of breast cancer patients immediately after surgery and before adjuvant therapy. Methods: Fourteen newly operated breast cancer patients were included in the study. Lactate dehydrogenase and superoxide dismutase activity were estimated using standard tests for evaluation of enzyme activity. Results: Activity of lactate dehydrogenase was in normal range in all but one sample obtained from patient at clinical status IIIB. Superoxide dismutase activity was elevated in 11 out of 14 patients, and was 2 to 8 fold higher compare to control values. The highest activity of superoxide dismutase was found in samples of two patients at clinical status I. Conclusion: Determination of lactate dehydrogenase and superoxide dismutase activity might be useful in clinical follow up of breast cancer patients.Archive of Oncology. 01/2008;
