Publications (42) View all
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Article: Changes Caused by Fruit Extracts in the Lipid Phase of Biological and Model Membranes.
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ABSTRACT: The aim of the study was to determine changes incurred by polyphenolic compounds from selected fruits in the lipid phase of the erythrocyte membrane, in liposomes formed of erythrocyte lipids and phosphatidylcholine liposomes. In particular, the effect of extracts from apple, chokeberry, and strawberry on the red blood cell morphology, on packing order in the lipid hydrophilic phase, on fluidity of the hydrophobic phase, as well as on the temperature of phase transition in DPPC liposomes was studied. In the erythrocyte population, the proportions of echinocytes increased due to incorporation of polyphenolic compounds. Fluorimetry with a laurdan probe indicated increased packing density in the hydrophilic phase of the membrane in presence of polyphenolic extracts, the highest effect being observed for the apple extract. Using the fluorescence probes DPH and TMA-DPH, no effect was noted inside the hydrophobic phase of the membrane, as the lipid bilayer fluidity was not modified. The polyphenolic extracts slightly lowered the phase transition temperature of phosphatidylcholine liposomes. The studies have shown that the phenolic compounds contained in the extracts incorporate into the outer region of the erythrocyte membrane, affecting its shape and lipid packing order, which is reflected in the increasing number of echinocytes. The compounds also penetrate the outer part of the external lipid layer of liposomes formed of natural and DPPC lipids, changing its packing order.Food Biophysics 03/2011; 6(1):58-67. · 2.19 Impact Factor -
Article: Assessment of Phytotoxicity of α-Aminoalkanephosphonic Acids Derivatives
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ABSTRACT: Newly synthesized derivatives of α-aminoalkanephosphonic acids (aminophosphonates) differ in the substituents at the carbon, nitrogen, and phosphorus atoms. They modified in different degree the properties of cucumber (Cucumis sativus cv. Wisconsin) cotyledon membranes, physiological activity of some enzymes (guaiacol and pyrogallol peroxidases, and catalase), chlorophyll content, and cellular membrane lipid peroxidation. Most active modifiers were those possessing sufficiently long hydrocarbon substituents at the nitrogen atom (C10H21) or isopropyl chain at the phosphorus atom. The branched tertbutyl group at the carbon atom enhanced slightly the activities of peroxidases in contrast to hexane ring at the same position, which decreased them.Biologia Plantarum 01/2003; 46(3):467-470. · 1.97 Impact Factor -
Article: Biological activity of binary mixtures of 2,4-D with some aminophosphonates
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ABSTRACT: A series of cyclic and acyclic aminophosphonates was synthesized for agrochemical application. Thecompounds differed in the substituents at the carbon, nitrogen and phosphorus atoms. Their potentialbiological activity was checked by studying their hemolytic potency, since hemolysis of erythrocytesby various compounds was found earlier to be a good indicator of their pesticidal efficiency. Aseries of hemolytic experiments permitted us to check the pesticidal efficiency ofaminophosphonates and to determine what structural features of aminophosphonates are responsiblefor it.Parallelly, we studied the hemolytic efficiency of binary mixtures of the aminophosphonates withthe well-known herbicide 2,4-dichlorophenoxyacetic acid (2,4-D... The aim was to check if potentialbiological efficiencies could be enhanced in comparison with those found for individual componentsdue to interactions between aminophosphonates and 2,4-D. An analysis of the effects binarymixtures was carried out by constructing graphs for both components of binary mixtures that givethe same hemolysis (the isobole method).Journal of Applied Biomedicine. 01/2003; -
Article: Antioxidative Properties of Pyrrolidinium and Piperidinium Salts
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ABSTRACT: Two series of pyrrolidinium (PYA-n) and piperidinium (PPPA-n) bromides with incorpo-rated antioxidant function were synthesized. Both have hydrocarbon chains with odd number of the carbon atoms (n) ranging between 7 and 15. Pig erythrocytes (RBC) were used to study antioxidant activity of these compounds. They were incorporated into RBC membranes in sublytic (micromolar) concentrations and RBC were then subjected to UV radiation. It was found that all the salts used protected erythrocyte membranes against oxidation of mem-brane lipids. This protection increased with hydrocarbon chain length. Such effect may be the result of an incorporation of particular compounds to different depths into the lipid phase of RBC membrane depending on their chain length. Such possibility was checked by studies on fluidity changes induced by the compounds studied in ghost membranes by fluorimetric measurements. The measurements showed that pyrrolidinium bromides were slightly more effective in a protection of erythrocytes than the corresponding piperidinium ones. The possible reason of such behaviour may be the difference in lipophilicity between piperidine and pyrrolidine rings.01/2002; 57:344-347. -
Article: SYNTHESIS OF SOME NEW CYCLIC AMINOPHOSPHONATES AND THEIR PHYSIOLOGICAL ACTIVITIES
Phosphorus Sulfur and Silicon and the Related Elements 08/2001; Sulfur(and Silicon and the Related Elements):119-128. · 0.72 Impact Factor
