Jacinto Costa

Publications (8) View all

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  Available from: Jacinto Costa

  Article: 2013-Novel E6 e E7 oncogenes variantes of human papillomavirus type 31 in braziliam women with abnorma cervical cytology

  Marcus Vinicius, Aragão Batista, Sergio Crovella, Ana Pavla Almeida, Diniz Gurgel, Jacinto Costa, Silva Neto, Ivi Gonçalves, Soares Santos Serra, Carolina Maria Medeiros Do Amaral, Valdir Queiroz Balbino, Maria Tereza, Cartaxo Muniz, Antonio Carlos De Freitas
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  ABSTRACT: a b s t r a c t 32 HPV-31 has been widely described as an important oncogenic type, showing high incidence in worldwide 33 and especially in Northeastern Brazil. We sought to identify the presence of specific mutations in HPV-31 34 E6 and E7 oncogenes in women with abnormal cervical smear. We enrolled 150 gynecological patients 35 from Sergipe State, Northeastern Brazil. HPV screening was carried out by polymerase chain reaction 36 (MY09/11). E6 and E7 oncogenes were amplified with specific primers and sequenced. The sequences 37 obtained were aligned with the GenBank reference sequences in order to search for genetic variants. 38 We identified genetic variants in E6 and E7 sequences from HPV-31. Two new nucleotide changes in 39 E6 and E7 were described for the first time in this study. A novel mutation in E6 resulted in amino acid 40 change in a site belonging to T-cell epitope with MHC II binding activity. There was no significant differ-41 ence in the distribution of HPV-31 E6 and E7 variants when compared to all selected clinical/epidemio-42 logical characteristics. HPV-31 isolates have been clustered into three main groups called lineages A, B 43 and C. We describe new HPV-31 variants in Brazil, contributing to better understand the genomic diver-44 sity of these viruses. 45 Ó 2013 Published by Elsevier B.V. 46 47 48
  Infection Genetics and Evolution 03/2013; 10:S1567-1348(13). · 3.13 Impact Factor
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  Available from: Jacinto Costa

  Dataset: capa livro

  Jacinto da Costa Silva Neto
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  Available from: Jacinto Costa

  Article: Anti-diabetic activity of extract from Persea americana Mill. leaf via the activation of protein kinase B (PKB/Akt) in streptozotocin-induced diabetic rats.

  C R Lima, C F B Vasconcelos, J H Costa-Silva, C A Maranhão, J Costa, T M Batista, E M Carneiro, L A L Soares, F Ferreira, A G Wanderley
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  ABSTRACT: The leaves of Persea americana Mill. (Lauraceae) have been popularly used in the treatment of diabetes in countries in Latin America and Africa. To investigate the hypoglycaemic properties and to determine the molecular mechanism by which the hydroalcoholic extract of the leaves of Persea americana reduce blood glucose levels in streptozotocin (STZ)-induced diabetes in rats via the enzymatic pathway of protein kinase B (PKB/Akt). The hydroalcoholic extract of the leaves of Persea americana (0.15 and 0.3g/kg/day), vehicle and metformin (0.5g/kg/day) were administered orally to STZ-diabetic rats (n=7/group) for 4 weeks. Changes in body weight, food and water intake, fasting glucose levels and oral glucose tolerance were evaluated. Phosphorylation and the expression of PKB in the liver and soleus muscle were determined by Western blot. The hydroalcoholic extract of the leaves of Persea americana reduced blood glucose levels and improved the metabolic state of the animals. Additionally, PKB activation was observed in the liver and skeletal muscle of treated rats when compared with untreated rats. The results indicate that the hydroalcoholic extract of the leaves of Persea americana has anti-diabetic properties and possibly acts to regulate glucose uptake in liver and muscles by way of PKB/Akt activation, restoring the intracellular energy balance.
  Journal of ethnopharmacology 03/2012; 141(1):517-25. · 2.32 Impact Factor
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  Available from: Jacinto Costa

  Article: Hypoglycaemic activity and molecular mechanisms of Caesalpinia ferrea Martius bark extract on streptozotocin-induced diabetes in Wistar rats.

  C F B Vasconcelos, H M L Maranhão, T M Batista, E M Carneiro, F Ferreira, J Costa, L A L Soares, M D C Sá, T P Souza, A G Wanderley
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  ABSTRACT: The tea from the stem bark of Caesalpinia ferrea Martius (Leguminosae) has been popularly used in the treatment of diabetes in Brazil. To investigate the hypoglycaemic properties and to elucidate the mechanisms by which the aqueous extract of the stem bark of Caesalpinia ferrea reduces blood glucose levels in streptozotocin-induced diabetic rats via the enzymatic pathways of protein kinase B (PKB/Akt), AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC). The aqueous extract of the stem bark of Caesalpinia ferrea (300 and 450 mg/kg/day), vehicle and metformin (500 mg/kg/day) were administered orally to STZ-diabetic rats (n = 7/group) for 4 weeks. Changes in body weight, food and water intake, fasting glucose levels and oral glucose tolerance were evaluated. Phosphorylation (P) and the expression of Akt, AMPK and ACC in the liver and skeletal muscle were determined using Western blot. The aqueous extract of the stem bark of Caesalpinia ferrea reduced blood glucose levels and improved the metabolic state of the animals. P-Akt was increased in the liver and skeletal muscle of the treated animals, P-AMPK was reduced only in the skeletal muscle of these animals and P-ACC was reduced in both when compared with untreated rats. The results indicate that the aqueous extract of the stem bark of Caesalpinia ferrea has hypoglycaemic properties and possibly acts to regulate glucose uptake in liver and muscles by way of Akt activation, restoring the intracellular energy balance confirmed by inhibition of AMPK activation.
  Journal of ethnopharmacology 09/2011; 137(3):1533-41. · 2.32 Impact Factor
• Article: Hypoglycaemic activity and molecular mechanisms of Caesalpinia ferrea Martius bark extract on streptozotocin-induced diabetes in Wistar rats

  C.F.B. Vasconcelos, H.M.L. Maranhão, T.M. Batista, E.M. Carneiro, F. Ferreira, J. Costa, L.A.L. Soares, M.D.C. Sá, T.P. Souza, A.G. Wanderley
  Journal of Ethnopharmacology. 137(3):1533-1541.