Hideyo Takatsuki

Publications (25) View all

• Article: Control of Myosin Motor Activity by the Reversible Alteration of Protein Structure for Application in a Bionanodevice

  Siva K Nalabotu, Hideyo Takatsuki, Madhukar Kolli, Leslie Frost, Benjamin Crowder, Shinji Yoshiyama, Murali Gadde, Sunil Kakarla, Kazuhiro Kohama, Arun Kumar, Eric R Blough
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  ABSTRACT: The application of bionanomotors for the transport of nano-sized objects and molecular sorting is an area of growing interest. Bionanomotors are motor proteins that have capability to convert chemical form of energy into mechanical energy with a remarkable degree of efficiency. Myosin II is a motor protein that has crucial role in muscle contraction, cell division and transport of cellular traffic. The use of the myosin II bio-nanomotor for nanotechnological applications has been hampered by an inability to modulate enzyme activity in a controlled fashion. Herein we examine if inducing changes in myosin motor structure can be used to regulate motor activity. Our findings indicate that myosin motor activity can be effectively and repeatedly cycled by altering the oxidative status of the experimental environment and that this means of control is most likely due to the formation of disulfide bonds between two adjacent myosin essential light chains. This strategy, if refined further, may prove useful for harnessing the potential of the myosin bionanomotor for future ex vivo applications.
  Advanced Science Letters. 09/2012; 16(1):213-221.
• Article: Transport of single cells using an actin bundle-myosin bionanomotor transport system.

  Hideyo Takatsuki, Hideyuki Tanaka, Kevin M Rice, Madhukar B Kolli, Siva K Nalabotu, Kazuhiro Kohama, Parviz Famouri, Eric R Blough
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  ABSTRACT: The potential of using actin bundles for the transport of liposomes and single cells across myosin-coated surfaces is investigated. Compared to that observed with filamentous actin, the liposome transport using actin bundles was more linear in nature and able to occur over longer distances. Bundles, but not filamentous actin, were capable of moving single cells. Cargo unloading from bundles was achieved by incubation with Triton X-100. These data suggest that actin bundling may improve the ability of the myosin motor system for nanotransport applications.
  Nanotechnology 06/2011; 22(24):245101. · 3.98 Impact Factor
• Article: Application of poly(amidoamine) dendrimers for use in bionanomotor systems.

  Madhukar B Kolli, B Scott Day, Hideyo Takatsuki, Siva K Nalabotu, Kevin M Rice, Kazuhiro Kohama, Murali K Gadde, Sunil K Kakarla, Anjaiah Katta, Eric R Blough
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  ABSTRACT: The study and utilization of bionanomotors represents a rapid and progressing field of nanobiotechnology. Here, we demonstrate that poly(amidoamine) (PAMAM) dendrimers are capable of supporting heavy meromyosin dependent actin motility of similar quality to that observed using nitrocellulose, and that microcontact printing of PAMAM dendrimers can be exploited to produce tracks of active myosin motors leading to the restricted motion of actin filaments across a patterned surface. These data suggest that the use of dendrimer surfaces will increase the applicability of using protein biomolecular motors for nanotechnological applications.
  Langmuir 03/2010; 26(9):6079-82. · 4.19 Impact Factor
• Article: Utilization of myosin and actin bundles for the transport of molecular cargo.

  Hideyo Takatsuki, Kevin M Rice, Shinichi Asano, B Scott Day, Mizuki Hino, Kazuhiro Oiwa, Ryoki Ishikawa, Yuichi Hiratsuka, Taro Q P Uyeda, Kazuhiro Kohama, Eric R Blough
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  ABSTRACT: The utilization of motor proteins for the movement and assembly of synthetic components is currently a goal of nanoengineering research. Application of the myosin actin motor system for nanotechnological uses has been hampered due to the low flexural rigidity of individual F-actin filaments. Here it is demonstrated how actin bundling can be used to affect the translational behavior of myosin-propelled filaments, transport molecules across a motor-patterned surface, and that the movement of bundled actin can be regulated photonically. These data suggest that actin bundling may significantly improve the applicability of the myosin motor for future nanotechnological applications.
  Small 02/2010; 6(3):452-7. · 8.35 Impact Factor
• Article: Assembly and Function of Myosin II on Ultraviolet/Ozone Patterned Trimethylchlorosilane Substrates

  Hideyo Takatsuki, Madhukar Kolli, Kevin Rice, B Scott Day, Shinichi Asano, Mashiur Rahman, Yue Zhang, Ryoki Ishikawa, Kazuhiro Kohama, Eric R Blough
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  ABSTRACT: The study of biomolecular motors represents a rapidly and progressing field of nanobiotechnology. Here, we present a simple method for patterning myosin II on a microstructured surface. Our findings indicate that UV/ozone treatment can be used to alter the hydrophobicity of trimethyl-chloro-silane (TMCS) coated glass surfaces, to alter protein binding, and effectively produce localized motor activity. Taken together, these data suggest that photoreactive patterning may be useful for the selective localization of functional myosin II motor tracks.
  Journal of Bionanoscience 06/2008; 2(1):35-41.