Haoyang Jiang

Questions and Answers (7) View all

• Question asked in PEI DNA Transfection
  Open How could it get turbid when I mingle PEI with DNA?
  I have tried to make the PEI-DNA complex with mole ratio of PEI and DNA as 1:1, 2:1, 4:1, 8:1, 12:1, 16:1, 20:1, 24:1 and 28:1. The concentration of P... [more]
  I have tried to make the PEI-DNA complex with mole ratio of PEI and DNA as 1:1, 2:1, 4:1, 8:1, 12:1, 16:1, 20:1, 24:1 and 28:1. The concentration of PEI and DNA is 2mg/ml and 2mg/ml, respectively. I found when I mingle them, the solution is still clear. However, after a while(not more than 15 min), most of them would be turbid in different degrees. When I am evaluating the diameter of the particles, they are usually more than 300nm and it may be increased with time if you try to evaluate more times. I have checked everything I used and I could promise that nothing is contaminated. But how could the solution get turbid?
  By Haoyang Jiang · Nanjing University
  Following
• Question asked in Transfection
  7 How could a large portion of GFP transfected in cells be overexposed under the confocal?
  Recently, I tried to transfect adipose-derived stem cells with GFP plasmid to detect the efficiency of the transfection. However, when I checked them... [more]
  Recently, I tried to transfect adipose-derived stem cells with GFP plasmid to detect the efficiency of the transfection. However, when I checked them under the confocal in 1 and 2 days after the transfection, I could see a lot of cells with GFP overexposure although some other cells have more nomal and dim green light. How could this happen? Are those cells with GFP overexposure dead?
  By Haoyang Jiang · Nanjing University
  Following
• Answer added in Cell Biology
  30 How could I evaluate the number of cells with protocols other than MTT or CCK8?
  By Haoyang Jiang · Nanjing University
  Haoyang Jiang · Nanjing University

  Thanks a lot, I would have a try. 

  Thanks a lot, I would have a try.
  Following
• Answer added in Cell Biology
  30 How could I evaluate the number of cells with protocols other than MTT or CCK8?
  By Haoyang Jiang · Nanjing University
  Haoyang Jiang · Nanjing University

  Ok. 

  Ok.
  Following
• Answer added in Cell Biology
  30 How could I evaluate the number of cells with protocols other than MTT or CCK8?
  By Haoyang Jiang · Nanjing University
  Haoyang Jiang · Nanjing University

  Thanks a lot. But I'm afraid I have to evaluate them immediately. Anyway, I would have a try. 

  Thanks a lot. But I'm afraid I have to evaluate them immediately. Anyway, I would have a try.
  Following