Publications (142) View all
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Article: Grecocyclines: new angucyclines from Streptomyces sp. Acta 1362
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ABSTRACT: Two novel angucyclines were isolated from the streptomycete Acta 1362. The strain was of particular interest regarding the production of characteristic metabolites that were detected by HPLC–diode array profiling of the extracts. Grecocycline A and B were isolated and their structures were determined. Grecocycline A shows cytotoxic activity and grecocycline B inhibits protein tyrosine phosphatase 1B. Moreover, shunt product grecocyline C was isolated and its structure was determined.European Journal of Organic Chemistry 04/2010; 2010(12):2344-2350. · 3.33 Impact Factor -
Article: Nikkomycins: Microbial inhibitors of chitin synthase
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ABSTRACT: Production of nikkomycins by Streptomyces tendae is dependent on phosphate concentration, pH and the presence of precursors or regulatory factors in the medium. In a batch-feeding system the production of antibiotically active nikkomycins could be increased up to 3 g dm−3. The selective production of single compounds was possible by feeding precursors or using mutants of Streptomyces tendae. The whole qualitative and quantitative determination of nikkomycins was carried out with ion-pair reversed-phase high-performance liquid chromatography (h.p.l.c.).Journal of Chemical Technology and Biotechnology. 04/2007; 32(1):271 - 280. -
Article: Biosynthetic gene cluster of simocyclinone, a natural multihybrid antibiotic.
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ABSTRACT: The entire simocyclinone biosynthetic cluster (sim gene cluster) from the producer Streptomyces antibioticus Tü6040 was identified on six overlapping cosmids (1N1, 5J10, 2L16, 2P6, 4G22, and 1K3). In total, 80.7 kb of DNA from these cosmids was sequenced, and the analysis revealed 49 complete open reading frames (ORFs). These ORFs include genes responsible for the formation and attachment of four different moieties originating from at least three different pools of primary metabolites. Also in the sim gene cluster, four ORFs were detected that resemble putative regulatory and export functions. Based on the putative function of the gene products, a model for simocyclinone D8 biosynthesis was proposed. Biosynthetic mutants were generated by insertional gene inactivation experiments, and culture extracts of these mutants were analyzed by high-performance liquid chromatography. Production of simocyclinone D8 was clearly detectable in the wild-type strain but was not detectable in the mutant strains. This indicated that indeed the sim gene cluster had been cloned.Antimicrobial Agents and Chemotherapy 06/2002; 46(5):1174-82. · 4.84 Impact Factor -
Article: Bagremycin A and B, novel antibiotics from streptomyces sp. Tü 4128.
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ABSTRACT: Two novel secondary metabolites, bagremycin A (2) and B (3), were detected in the culture filtrate of Streptomyces sp. Tü 4128 by HPLC-diode-array screening. They are phenol esters of 3-amino-4-hydroxybenzoic acid with a derivative of p-coumaric acid and show a moderate activity against gram-positive bacteria and some fungi.The Journal of Antibiotics 10/2001; 54(9):730-6. · 1.65 Impact Factor -
Article: Optical biosensors. Monitoring studies of glycopeptide antibiotic fermentation using white light interference.
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ABSTRACT: This paper describes the design, characterization, and use of an optical biosensor suited for the process control of biotechnological processes. The detector principle is based on reflectometric interference spectroscopy (RIfS). RIfS enables a label-free, product-specific monitoring, with a future outline for on-line process control. The potential of the RIfS biosensor is exemplified by the qualitative and quantitative monitoring of the microbial production of vancomycin-type glycopeptide antibiotics.Analytical Chemistry 10/2001; 73(17):4313-8. · 5.86 Impact Factor
