Marta Wlodarczyk, Grazyna Nowicka[show abstract] [hide abstract]
ABSTRACT: Genetic heterogeneity influencing enzyme activity may change the capacity to repair DNA damage induced by environmental and endogenous factors. This study aims to assess the impact of Lys751Gln (A/C) polymorphism in the XPD gene, encoding an enzyme involved in the nucleotide excision repair pathway, on individual DNA damage. The DNA damage in human lymphocytes (% DNA in the tail) was quantified by means of single-cell gel electrophoresis. Baseline levels of DNA damage significantly differ between AA homozygotes and carriers of the C allele, and the observed differences were not related to age, gender, or smoking status. It seems that the AA variant is associated with enhanced protection against oxidative DNA damage.Biochemical Genetics 07/2012; · 0.86 Impact Factor
Article: Common polymorphisms in CYP1A1, GSTM1, GSTT1, GSTP1 and XPD genes and endogenous DNA damage.Marta Wlodarczyk, Grazyna Nowicka[show abstract] [hide abstract]
ABSTRACT: Endogenous DNA damage levels were analyzed in relation to polymorphisms in genes encoding phase I detoxifying enzyme-CYP1A1, phase II detoxifying enzymes-GSTM1, GSTT1, GSTP1 and enzyme involved in nucleotide excision repair-XPD. The study group consisted of 220 healthy non-smoking volunteers; 90 men and 130 woman, 25-60 years old (44 ± 10 years). The level of DNA damage (% DNA in tail) was evaluated by alkaline comet assay. The genetic variants were determined by restriction fragment length polymorphism PCR. The highest level of DNA damage (6.7%) was found in carriers of both: AA variant of XPD gene and M1 null variant of GSTM1 gene. The lowest level of DNA breaks (3.7%) was associated with the genotype GSTP1-AA/GSTM1 (+).Molecular Biology Reports 12/2011; 39(5):5699-704. · 2.93 Impact Factor
Article: [Dietary intake of n-3 polyunsaturated fatty acids in healthy adults in relation to current recommended intake].[show abstract] [hide abstract]
ABSTRACT: The health benefits of n-3 PUFA and especially long-chain n-3 PUFA are well recognized and recommendation of their dietary intake for general population are elaborated However, there is still a need for assessment of LCn-3 PUFA intake in different population groups. The aim of this study was to assess intake of n-3 PUFA, particularly long-chain n-3PUFA (LCn-3 PUFA) and to identify their major sources in diets of healthy subjects. The studied group consisted of 182 adults, both men and women. Assessment of n-3 PUFA dietary intake was based on individual 3-day records. Data were analyzed using updated polish food composition tables and "Dieta 2" and "Dieta 4" Software. It was found, that more than 40% of studied subjects consumed daily less than 1 g ALA, about 50%--less than 100 mg LCn-3 PUFA and about 60% less than 10 mg DHA.Roczniki Państwowego Zakładu Higieny 01/2011; 62(4):389-96.
Agnieszka Jarosz, Grazyna Nowicka[show abstract] [hide abstract]
ABSTRACT: Atherosclerosis is a chronic disorder with a complex pathology. Apart from classical risk factors such as: hypertension, elevated serum lipids, cigarette smoking, diabetes and obesity, other new risk factors involved in atherosclerosis development have been discovered. These new factors include molecules associated with inflammation such as C-reactive protein (CRP), and also eleveted homocysteine (Hcy). To estimate the frequency of enhanced CRP and Hcy levels in two groups of patients: in patients with diagnosed coronary heart disease (CHD group) and without CHD symptoms (control), matched for serum cholesterol levels, age, sex, body mass index and blood pressure. 335 subjects: 182 females, 153 males, aged between 42-63. In all studied subjects serum levels of total and HDL-cholesterol, triglycerides, Hcy and CRP levels were determined. HCY-levels lower than 10 mmol/l were observed in 53.3% of the controls and in 27.7% of the group diagnosed with coronary heart disease. Hcy above 15 mmol/l was observed in 29.7% of CHD group and in only 5% of the controls. 78.5% of controls had CRP levels concentrations not higher than 2 mg/l. CRP levels above 3 mg/l were observed only in 9.9% of controls and in up to 66.5% of the patients with coronary heart disease. CRP and Hcy levels have been recognized as parameters that strongly differentiate patients with classically diagnosed heart disease from people without clinical symptoms of the illness but with similar serum lipid levels, similar BMI and blood pressure.Przegla̧d lekarski 02/2008; 65(6):268-72.
Article: [New European guidelines on cardiovascular disease prevention in relation to the situation in Poland].Wiktor B Szostak, Barbara Cybulska, Longina Kłosiewicz-Latoszek, Grazyna Nowicka, Dorota Szostak-WegierekKardiologia polska 01/2005; 61(12):624-7. · 0.51 Impact Factor