Grażyna Adler

Publications (33) View all

• Article: Prevalence of 1691G>A FV mutation in females from Bosnia and Herzegovina - a preliminary report.

  Amina Valjevac, Bakir Mehić, Emina Kiseljaković, Slavka Ibrulj, Agnieszka Garstka, Grażyna Adler
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  ABSTRACT: Factor V is the liver-synthesized multidomain glycoprotein encoded by a gene localised on chromosome 1q23. The point mutation 1691G>A in this gene results in formation of an altered protein of V Factor resistant to activated protein C (APC) cleavage. This mutation alone is the most frequent cause of inborn thrombophilia and the most widely acknowledged genetic risk factor for venous thrombosis in a Caucasian population. This study was designed to provide the first estimate of the frequency of the allele 1691A FV in the Bosnian female population. The 1691G>A FV mutation was examined by polymerase chain reaction-restriction fragment length polymorphism, in a group of 67 women, mean age of 58.6 years with no history of cardiovascural incident. Our findings revealed an absence of the mutated allele 1691A FV in the studied group. This is the first report on the 1691G>A FV mutation in a population from Bosnia and Herzegovina. Further research is needed to establish prevalence of the mutated allele in the population from Bosnia and Herzegovina.
  Bosnian journal of basic medical sciences / Udruzenje basicnih mediciniskih znanosti = Association of Basic Medical Sciences 02/2013; 13(1):31-3. · 0.25 Impact Factor
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  Available from: Grażyna Adler

  Article: CYP17 and CYP19 genetic variants are not associated with age at natural menopause in Polish women.

  Bogdan Rumianowski, Grażyna Adler, Krzysztof Safranow, Agnieszka Brodowska, Beata Karakiewicz, Sylwia Słuczanowska-Głąbowska, Beata Loniewska, Małgorzata Piasecka, Andrzej Ciechanowicz, Maria Laszczyńska
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  ABSTRACT: The aim of the study was to investigate associations between two common polymorphisms of CYP17 and CYP19, encoding key enzymes of estrogen biosynthesis, and age at menopause in Polish women. One hundred fifty women after menopause (49.5±3.8 years), with no previous history of hormone replacement therapy took part in the study. The genetic control group consisted of 150 newborns from the same population. We investigated an association between the age at menopause and the single nucleotide polymorphism T→C in the 5' untranslated region (promoter) of the CYP17 gene (c.-34T>C; rs743572 - MspA1) or the number of tetranucleotide repeats [TTTA](n) (rs60271534) including deletion/insertion (D/I) of a 3bp sequence in intron 4 of the CYP19 gene. CYP17 polymorphism was analyzed by PCR-RFLP and CYP19 by PCR and capillary electrophoresis. In the case of CYP17 polymorphism, 28.7% and 36.7% wild homozygous (TT), 50.7% and 46.0% heterozygous (TC), as well as 20.6% and 17.3% mutated homozygous (CC) types were identified in the subjects and controls, respectively. The frequency of mutated alleles (C) was 46.0% vs. 40.3% (p=0.19). In the case of CYP19 polymorphism, 34.0% and 32.0% of homozygotes (1_1), 50.7% and 51.3% of heterozygotes (1_2), 15.3% and 16.7% of homozygotes (2_2) were identified in the subjects and controls, respectively. No association between the studied CYP17 or CYP19 polymorphisms and age at menopause was found in Polish women.
  Reproductive biology 12/2012; 12(4):368-73. · 1.92 Impact Factor
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  Article: 1936A→G (I646 V) Polymorphism in the AKAP10 Gene Encoding A-Kinase-Anchoring Protein 10 in Very Long-Lived Poles is Similar to that in Newborns.

  Beata Loniewska, Grażyna Adler, Janusz Gumprecht, Władysław Grzeszczak, Mariusz Kaczmarczyk, Agnieszka Bińczak-Kuleta, Andrzej Ciechanowicz, Jeremy S Clark
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  ABSTRACT: Background/Study Context: The common 1936A→G transition (rs203462) in the AKAP10 gene encoding the A-kinase-anchoring protein 10 has been recently associated with negative prognosis in the aging European American population (60 to 79 years old). The aim of this study was to see the effects of this transition on allele frequency in very long-lived Poles. Methods: AKAP10 genotype and allele distributions were analyzed in Polish subjects: 148 nonagenarians (95 to 103 years old) and 200 healthy newborn controls, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Distributions were separated according to gender and χ(2) tests used to analyze possible differences. Results: No significant differences were found in genotype or allele distribution between the age groups, for either gender. Percentages of GG AKAP10 homozygotes were slightly greater in the very old subjects than in the newborns (12.2% vs. 9.0%, respectively), and the G allele percentages were very similar (males, 30.7% and 33.0%; females, 34.1% and 35.8%; respectively). Conclusion: The authors conclude that differences in study results between European Americans (60 to 79 years old) and Poles (≥95 years old) result from either (1) geographical location; or (2) the influence of this polymorphism on groups of people differing in genetic background or environmental history; or (3) the time window affected, including extreme age. Further studies with full age-frequency distributions are needed to clarify these results.
  Experimental Aging Research 10/2012; 38(5):584-92. · 1.31 Impact Factor
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  Available from: Grażyna Adler

  Article: Association of 1936A > G in AKAP10 (A-kinase anchoring protein 10) and blood pressure in Polish full-term newborns.

  Beata Loniewska, Mariusz Kaczmarczyk, Jeremy S Clark, Agnieszka Bińczak-Kuleta, Grażyna Adler, Agnieszka Kordek, Anita Horodnicka-Józwa, Grażyna Dawid, Jacek Rudnicki, Andrzej Ciechanowicz
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  ABSTRACT: Objective. The 1936G AKAP10 allele is associated with increased adult basal heart rate (HR) and decreased variability, markers of low cholinergic/vagus sensitivity associated with hypertension. Blood pressure (BP) values in newborns are important measurable markers of cardiovascular risk later in life. The question was whether decreased vagal function-related 1936A > G AKAP10 is associated with newborn BP. Study design. 114 healthy Polish newborns born after 37th gestational week to healthy women with uncomplicated pregnancies. At birth, newborn cord blood obtained for isolation of genomic DNA. BP and HR measured on days 1 and 3 after delivery. Results. Diastolic BP on day 3 and absolute and relative differences between diastolic BP values, as well as between mean BP values on day 3 and on day 1 after birth, in carriers of 1936G AKAP10 allele, were significantly higher as compared with wild-type homozygotes. Conclusion. Results demonstrate possible association between 1936G AKAP10 variant and BP in Polish newborns.
  Blood pressure 07/2012; · 1.26 Impact Factor
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  Available from: Grażyna Adler

  Article: The effect of preservation solutions UW and EC on the expression of renin I, angiotensinogen and angiotensin I-converting enzyme genes in rat kidney.

  Tadeusz Sulikowski, Leszek Domanski, Zbigniew Zietek, Grazyna Adler, Andrzej Pawlik, Mariusz Kaczmarczyk, Andrzej Ciechanowicz, Kazimierz Ciechanowski, Marek Ostrowski
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  ABSTRACT: Ischemia/reperfusion injury in organ transplantation is a multifactor process that may lead to delayed graft function (DGF), and has a significant impact on short- and long-term graft survival. The activation of the renin-angiotensin system may be important in the pathophysiology of DGF. Preservation solutions are thought to diminish the ischemic injury, and appropriate choice of the solution should contribute to improved graft function and better prognosis for graft survival. The aim of this study was to examine the effect of preservation solutions UW and EC on the expression of renin I, angiotensinogen and angiotensin I-converting enzyme genes in rat kidney. The study was carried out on Wistar rat kidneys divided into 3 groups: kidneys perfused with 0.9% NaCl (control group), with UW preservation solution, and with EC preservation solution. We investigated the expressions of renin I, angiotensinogen- and angiotensin I-converting enzyme genes in kidneys perfused with EC and UW solutions after 12 min (minutes) and 24 h (hours) of cold ischemia and 30 min of warm ischemia. The perfusion with UW and EC solution caused an increase of renin I, angiotensinogen and angiotensin I-converting enzyme genes expression in kidneys. This increase was enhanced in kidneys perfused with UW solution in comparison with kidneys perfused with EC solution. The 24 h preservation with UW solution resulted in a decrease of renin-angiotensin activity increased in cold ischemia. UW preservation of 24 h decreased renin-angiotensin system activity activated in cold ischemia but not in warm ischemia.
  Annals of transplantation: quarterly of the Polish Transplantation Society 09/2011; 16(3):108-13. · 2.02 Impact Factor