Francis Galibert

Publications (315) View all

• Article: Activation of SRE and AP1 by olfactory receptors via the MAPK and Rho dependent pathways.

  Naïma Benbernou, Stéphane Esnault, Francis Galibert
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  ABSTRACT: Whereas the activation of MAPKs (mitogen activated kinases) and Rho dependant pathways by GPCR (G protein coupled receptors) has been the subject of many studies, its implication in the signalling of olfactory receptors, which constitute the largest GPCR family, has been far less analysed. Using an in vitro heterologous system, we showed that odorant activated ORs activate SRE containing promoters via the ERK pathway. We also demonstrated that RhoA and Rock kinases but not Rac were involved in ORs-induced SRE/SRF activation and that AP1 was activated, via JNK and p38 MAPKinase. Using real time PCR we found that mOR23, RnI7 and CfOR12A07 induced elevated levels of transcription factors ELK-4, srf, c-fos and c-jun mRNAs whereas mOREG induced an elevated transcription levels of c-fos and c-jun mRNA only. We showed also that odorant activated ORs stimulate the downstream MAPKs and Rho pathways in primary cultures of rat olfactory sensory neurons (OSNs). Similar results were also obtained with OE (olfactory epithelium) extracts prepared from rats exposed to odorants in vivo. Finally, we showed the important role of the AKT and MAPK signalling pathways in OSNs survival. Taken together, these data provide direct evidence that the binding of odorants onto their ORs activate the MAPK and Rho signalling pathways that are involved in OSNs survival events. This suggests that these pathways could be implicated in the regulation of OSNs homeostasis.
  Cellular signalling 03/2013; · 4.09 Impact Factor
• Source

  Available from: Nicole Stange-Thomann

  Dataset: nature04338-s2

  Kerstin Lindblad-Toh, Claire M Wade, Tarjei S. Mikkelsen, Elinor K. Karlsson, David B. Jaffe, Michael Kamal, Michele Clamp, Jean L. Chang, Edward J. Kulbokas, Michael C. Zody, [......], Leanne Hughes, Leigh Hunnicutt, M. Husby, Benjamin Jester, Charlien Jones, Asha Kamat, Ben Kanga, Cristyn Kells, Dmitry Khazanovich, Alix Chinh Kieu
• Source

  Available from: Nicole Stange-Thomann

  Dataset: nature04338-s3

  Kerstin Lindblad-Toh, Claire M Wade, Tarjei S. Mikkelsen, Elinor K. Karlsson, David B. Jaffe, Michael Kamal, Michele Clamp, Jean L. Chang, Edward J. Kulbokas, Michael C. Zody, [......], Leanne Hughes, Leigh Hunnicutt, M. Husby, Benjamin Jester, Charlien Jones, Asha Kamat, Ben Kanga, Cristyn Kells, Dmitry Khazanovich, Alix Chinh Kieu
• Source

  Available from: Nicole Stange-Thomann

  Dataset: nature04338-s1-1

  Kerstin Lindblad-Toh, Claire M Wade, Tarjei S. Mikkelsen, Elinor K. Karlsson, David B. Jaffe, Michael Kamal, Michele Clamp, Jean L. Chang, Edward J. Kulbokas, Michael C. Zody, [......], Leanne Hughes, Leigh Hunnicutt, M. Husby, Benjamin Jester, Charlien Jones, Asha Kamat, Ben Kanga, Cristyn Kells, Dmitry Khazanovich, Alix Chinh Kieu
• Source

  Available from: Francis Galibert

  Article: A high-resolution map of the Nile tilapia genome: a resource for studying cichlids and other percomorphs.

  Richard Guyon, Michaelle Rakotomanga, Naoual Azzouzi, Jean Pierre Coutanceau, Celine Bonillo, Helena D'Cotta, Elodie Pepey, Lucile Soler, Marguerite Rodier-Goud, Angelique D'Hont, Matthew A Conte, Nikkie Em van Bers, David J Penman, Christophe Hitte, Richard Pma Crooijmans, Thomas D Kocher, Catherine Ozouf-Costaz, Jean Francois Baroiller, Francis Galibert
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  ABSTRACT: The Nile tilapia (Oreochromis niloticus) is the second most farmed fish species worldwide. It is also an important model for studies of fish physiology, particularly because of its broad tolerance to an array of environments. It is a good model to study evolutionary mechanisms in vertebrates, because of its close relationship to haplochromine cichlids, which have undergone rapid speciation in East Africa. The existing genomic resources for Nile tilapia include a genetic map, BAC end sequences and ESTs, but comparative genome analysis and maps of quantitative trait loci (QTL) are still limited. We have constructed a high-resolution radiation hybrid (RH) panel for the Nile tilapia and genotyped 1358 markers consisting of 850 genes, 82 markers corresponding to BAC end sequences, 154 microsatellites and 272 single nucleotide polymorphisms (SNPs). From these, 1296 markers could be associated in 81 RH groups, while 62 were not linked. The total size of the RH map is 34,084 cR3500 and 937,310 kb. It covers 88% of the entire genome with an estimated inter-marker distance of 742 Kb. Mapping of microsatellites enabled integration to the genetic map. We have merged LG8 and LG24 into a single linkage group, and confirmed that LG16-LG21 are also merged. The orientation and association of RH groups to each chromosome and LG was confirmed by chromosomal in situ hybridizations (FISH) of 55 BACs. Fifty RH groups were localized on the 22 chromosomes while 31 remained small orphan groups. Synteny relationships were determined between Nile tilapia, stickleback, medaka and pufferfish. The RH map and associated FISH map provide a valuable gene-ordered resource for gene mapping and QTL studies. All genetic linkage groups with their corresponding RH groups now have a corresponding chromosome which can be identified in the karyotype. Placement of conserved segments indicated that multiple inter-chromosomal rearrangements have occurred between Nile tilapia and the other model fishes. These maps represent a valuable resource for organizing the forthcoming genome sequence of Nile tilapia, and provide a foundation for evolutionary studies of East African cichlid fishes.
  BMC Genomics 06/2012; 13:222. · 4.07 Impact Factor