Fernando Cardoso

Questions and Answers (90) View all

• Answer added in Plant Biotechnology
  33 Sterilization of Arabidopsis seeds?
  By Kasturi Banerjee · Kyushu University
  Fernando Cardoso · Universidade NOVA de Lisboa

  I do a 1st wash with etanol 96%, for 1 min to remove wax and them the Mohammad Ali protocol. 

  I do a 1st wash with etanol 96%, for 1 min to remove wax and them the Mohammad Ali protocol.
  Following
• Answer added in Immunological Assays
  9 Can I re-incubate ELISA plates with primary Ab?
  By Rafael Brandão · University of Copenhagen
  Fernando Cardoso · Universidade NOVA de Lisboa

  You can re-use your antigen ( protein or antibody) just collected after first incubation and use again up to two times. But if you want you can al... [more]

  You can re-use your antigen ( protein or antibody) just collected after first incubation and use again up to two times. But if you want you can also re-use your ELISA plates: Add 0.1-0.4M NaOH or an alkaline detergent at 60ºC, for 20 min, them live overnight, next day wash with tap water, dry it and re-use it!.... with your first antibody! You can test if the plates are ok, just add second antibody in bloking buffer, wash then add substact and the OD value should be the same as (control plate) the new 96 well plate.
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• Answer added in ELISA
  5 Sandwich ELISA - nitrocellulose printing issues.
  By Valentin Romanov · Wasatch Microfluidics
  Fernando Cardoso · Universidade NOVA de Lisboa

  Dilute your conjugate more that the dilutions you made. 

  Dilute your conjugate more that the dilutions you made.
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• Answer added in Contamination
  82 Human cancer cell line culture contamination
  By Céline Gonçalves · Universidade do Minho
  Fernando Cardoso · Universidade NOVA de Lisboa

  John Schmitz, even if that is not mycoplasma, in my lab I have two Vero cultures one with that "comtamination" and another without that! and the cult... [more]

  John Schmitz, even if that is not mycoplasma, in my lab I have two Vero cultures one with that "comtamination" and another without that! and the culture medium is the same. Even if he culture in blood agar that contamination eventually could not growth without the mamalian cells that can excrete some compounds to the medium...
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