Fausto Sánchez-Muñoz

Publications (23) View all

• Article: Vanin-1 as a potential novel biomarker for active nephritis in systemic lupus erythematosus.

  F Sánchez-Muñoz, Lm Amezcua-Guerra, M Macías-Palacios, R Márquez-Velasco, R Bojalil
  Lupus 02/2013; · 2.34 Impact Factor
• Article: Anti-tumor necrosis factor VNAR single domains reduce lethality and regulate underlying inflammatory response in a murine model of endotoxic shock.

  Rafael Bojalil, María Teresa Mata-González, Fausto Sánchez-Muñoz, Yepci Yee, Iván Argueta, Lucía Bolaños, Luis Manuel Amezcua-Guerra, Tanya Amanda Camacho-Villegas, Edna Sánchez-Castrejón, Walter Jakob García-Ubbelohde, Alexei Fedorovish Licea-Navarro, Ricardo Márquez-Velasco, Jorge Fernando Paniagua-Solís
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  ABSTRACT: BACKGROUND: In sepsis, tumor necrosis factor (TNF) is the key factor triggering respiratory burst, tissue injury and disseminated coagulation. Anti-TNF strategies based on monoclonal antibodies or F(ab')2 fragments have been used in sepsis with contradictory results. Immunoglobulin new antigen receptors (IgNAR) are a unique subset of antibodies consisting of five constant (CNAR) and one variable domains (VNAR). VNAR domains are the smallest, naturally occurring, antibody-based immune recognition units, having potential use as therapy.Our aim was to explore the impact of an anti-TNF VNAR on survival in an experimental model of endotoxic shock. Also, mRNA expression and serum protein of several inflammatory molecules were measured. RESULTS: Endotoxic shock was induced by lipopolysaccharide (LPS) in male Balb/c mice. Animals were treated with anti-TNF VNAR domains, F(ab')2 antibody fragments, or saline solution 15 minutes before, 2h and 24h after lethal dose100 (LD100) LPS administration. TNF blockade with either VNAR domains or F(ab')2 fragments were associated with lower mortality (60% and 75%, respectively) compared to LD100. Challenge with LPS induced significant production of serum TNF and interleukins -10 and -6 at 3h. After that, significant reduction of IL-6 at 24h (vs 3h) was shown only in the VNAR group. Nitrites level also increased in response to LPS.In liver, TNF and IL-10 mRNA expression showed a pro-inflammatory imbalance in response to LPS. Blocking TNF was associated with a shift towards an anti-inflammatory status; however, polarization was more pronounced in animals receiving F(ab')2 fragments than in those with VNAR therapy. With regard to IL-6, gene expression was increased at 3h in all groups. TNF blockade was associated with rapid and sustained suppression of IL-6 expression, even more evident in the VNAR group. Finally, expression of inducible-nitric oxide synthase (iNOS) increased in response to LPS at 3h, but this was decreased at 24 h only in the anti-TNF VNAR group. CONCLUSIONS: Anti-TNF VNAR single domains improved survival in a murine model of endotoxic shock. Protection was associated with regulation in the TNF/IL-10 balance, attenuation of IL-6 and iNOS gene expression in the liver as well as decreased serum IL-6 concentration.
  BMC Immunology 04/2013; 14(1):17. · 2.53 Impact Factor
• Article: Association of the I148M/PNPLA3 Variant with Elevated Alanine Transaminase Levels in Normal-weight and Overweight/Obese Mexican Children.

  Elena Larrieta-Carrasco, Paola León-Mimila, Teresa Villarreal-Molina, Hugo Villamil-Ramírez, Sandra Romero-Hidalgo, Leonor Jacobo-Albavera, Roxana Gutiérrez-Vidal, Blanca E López-Contreras, Luz E Guillén-Pineda, Fausto Sánchez-Muñoz, Rafael Bojalil, Ana M Mejía-Domínguez, Nahúm Méndez-Sánchez, Aaron Domínguez-López, Carlos A Aguilar-Salinas, Samuel Canizales-Quinteros
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  ABSTRACT: BACKGROUND AND AIMS: Non-alcoholic fatty liver disease (NAFLD) and elevated alanine transaminase (ALT) levels are common in obese Hispanic adults and children. Recently, a PNPLA3 gene variant (I148M) was strongly associated with NAFLD and higher ALT levels in obese adults, including Hispanics. The aims of this study were to estimate the frequency of elevated ALT levels, and to address the influence of obesity and PNPLA3/I148M on ALT levels in a general population sample of Mexican school-aged children. METHODS: 1037 non-related Mexican children aged 6 to 12 years were genotyped for the I148M variant. Anthropometric, clinical and metabolic parameters were collected from all participants. RESULTS: Elevated ALT levels (>35 U/L) were more frequent in obese (26.9%) and overweight (9.3%) than in normal weight children (2.2%). The M148M genotype was significantly associated with elevated ALT levels in this population (OR=3.7, 95%CI 2.3-5.9; P=3.7x10(-8)), and children carrying the M148M genotype showed significantly lower HDL cholesterol levels and BMI z-core (P=0.036 and 0.015, respectively). On stratifying by BMI percentile, this genotype conferred a much greater risk of elevated ALT levels in normal weight (OR=19.9, 95%CI 2.5-157.7; P=0.005) than overweight and obese children (OR=3.4, 95%CI 1.3-8.9;P=0.014 and OR=3.1, 95%CI 1.7-5.5; P=1.4 x10(-4), respectively). CONCLUSIONS: The I148M PNPLA3 variant is strongly associated with elevated ALT levels in normal weight and overweight/obese Mexican children. Thus, the M148M genotype may be considered as an important risk factor for liver damage in this population.
  Gene 03/2013; · 2.34 Impact Factor
• Article: VNN1 Gene Expression Levels and the G-137T Polymorphism Are Associated with HDL-C Levels in Mexican Prepubertal Children.

  Leonor Jacobo-Albavera, Pablo I Aguayo-de la Rosa, Teresa Villarreal-Molina, Hugo Villamil-Ramírez, Paola León-Mimila, Sandra Romero-Hidalgo, Blanca E López-Contreras, Fausto Sánchez-Muñoz, Rafael Bojalil, Juan Antonio González-Barrios, Carlos A Aguilar-Salinas, Samuel Canizales-Quinteros
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  ABSTRACT: VNN1 gene expression levels and the G-137T polymorphism have been associated with high density lipoprotein cholesterol (HDL-C) levels in Mexican American adults. We aim to evaluate the contribution of VNN1 gene expression and the G-137T variant to HDL-C levels and other metabolic traits in Mexican prepubertal children. VNN1 mRNA expression levels were quantified in peripheral blood leukocytes from 224 unrelated Mexican-Mestizo children aged 6-8 years (107 boys and 117 girls) and were genotyped for the G-137T variant (rs4897612). To account for population stratification, a panel of 10 ancestry informative markers was analyzed. After adjustment for admixture, the TT genotype was significantly associated with lower VNN1 mRNA expression levels (P = 2.9 × 10(-5)), decreased HDL-C levels (β = -6.19, P = 0.028) and with higher body mass index (BMI) z-score (β = 0.48, P = 0.024) in the total sample. In addition, VNN1 expression showed a positive correlation with HDL-C levels (r = 0.220; P = 0.017) and a negative correlation with BMI z-score (r = -0.225; P = 0.015) only in girls. Our data suggest that VNN1 gene expression and the G-137T variant are associated with HDL-C levels in Mexican children, particularly in prepubertal girls.
  PLoS ONE 01/2012; 7(11):e49818. · 4.09 Impact Factor
• Article: High gene expression of MDR1 (ABCB1) is associated with medical treatment response and long-term remission in patients with ulcerative colitis.

  Jesús K Yamamoto-Furusho, Marco A Villeda-Ramírez, Gabriela Fonseca-Camarillo, Fausto Sánchez-Muñoz, Aarón Dominguez-Lopez, Rafael Barreto-Zuñiga, Misael Uribe
  Inflammatory Bowel Diseases 09/2009; 16(4):541-2. · 4.86 Impact Factor