Questions and Answers (4) View all
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Question asked in Plant Biotechnology3 Determination of trehalose in wheat with HPLCI wish to know which is the best available way to determine trehalose concentration in wheat plant?I wish to know which is the best available way to determine trehalose concentration in wheat plant?By Faheem Khan · Huazhong Agricultural UniversityFollowing
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Answer added in Plant Biotechnology6 Wheat transformationBy Faheem Khan · Huazhong Agricultural UniversityFaheem Khan · Huazhong Agricultural UniversityThanks everyone I will try your sugestionsThanks everyone I will try your sugestionsFollowing
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Answer added in Plant Biotechnology4 Ligation and transformationBy Tuyet Van · Crop BiotechnologyFaheem Khan · Huazhong Agricultural UniversityHi I am not very sure but I did ligation with takarra ligation kit d6020a and it works wonders I hope it will work as before I was facing the problem ... [more]Hi I am not very sure but I did ligation with takarra ligation kit d6020a and it works wonders I hope it will work as before I was facing the problem of not getting any positive colonies too....good luckFollowing
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Question asked in Plant Biotechnology6 Wheat transformationI know the best possible explant for transformation in wheat is immature embryos but the problem related to it is, it's too laborious and the time per... [more]I know the best possible explant for transformation in wheat is immature embryos but the problem related to it is, it's too laborious and the time period to isolate it is too limited. Can anyone help me know what can I do if I missed that crucial time for immature embryo isolation. Which explant I can use for transformation except immature or mature embryos in wheat?By Faheem Khan · Huazhong Agricultural UniversityFollowing