Ernesto Moro-Rodríguez
Research interests
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InterestsApoptosis, pre-neoplastic lesions, Experiment
Publications
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1.93Impact points
Postnatal maturation of the gastrointestinal tract: a functional and immunohistochemical study in the guinea-pig ileum at weaning.
Neuroscience letters. 10/2009;
Gastrointestinal motility is mainly controlled by the myenteric plexus. The longitudinal muscle - myenteric plexus (LMMP) preparation from the guinea-pig ileum is the best characterised adult gastrointestinal preparation; it has also been studied in old and neonatal animals, but not at weaning, when... [more] Gastrointestinal motility is mainly controlled by the myenteric plexus. The longitudinal muscle - myenteric plexus (LMMP) preparation from the guinea-pig ileum is the best characterised adult gastrointestinal preparation; it has also been studied in old and neonatal animals, but not at weaning, when milk is substituted with the food typical of adult animals. We used LMMP preparations from weanling and adult guinea-pigs to study different functional parameters and immunohistochemically identified subpopulations of myenteric neurones, including the excitatory motor neurones to the longitudinal muscle (LM-EMN). Excitatory stimuli (low-frequency electrical stimulation, acetylcholine, substance P, and naloxone in morphine-tolerant preparations) produced similar responses in weanling and adult guinea-pigs. The endogenous cannabinoid anandamide, but not the synthetic cannabinoid agonist WIN 55,212-2 or the opioid morphine, inhibited the electrically-stimulated twitches less efficaciously, and in vitro tolerance to morphine was also lower in weanling compared to adult animals. The packing densities of the calbindin-immunoreactive neurones (sensory neurones) and of neurones immunoreactive to both calretinin (CR) and neurofilament triplet protein (NFT; ascending interneurones) were slightly but significantly lower in weanling animals, whereas those of the neurones immunoreactive to CR but not NFT (LM-EMN) or immunoreactive to nitric oxide synthase (mainly inhibitory motor neurones) were comparable to the adult. Although guinea-pigs are relatively mature and can even ingest solid food at birth, their myenteric plexus is still not fully mature at the standard time of weaning. The nutritional, behavioural and environmental changes associated with weaning may be essential to attain full maturation of the myenteric plexus and gastrointestinal motility.
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1.30Impact points
Genotype distribution of cervical human papillomavirus DNA in women with cervical lesions in Bioko, Equatorial Guinea.
Diagnostic pathology. 10/2009; 4(1):31.
ABSTRACT: The HVP vaccine is a useful tool for preventing cervical cancer. The purpose of this study is to determine the most prevalent HPV genotypes in Equatorial Guinea in order to develop future vaccination strategies to apply in this country. A campaign against cervical cancer was carried out in... [more] ABSTRACT: The HVP vaccine is a useful tool for preventing cervical cancer. The purpose of this study is to determine the most prevalent HPV genotypes in Equatorial Guinea in order to develop future vaccination strategies to apply in this country. A campaign against cervical cancer was carried out in the area on a total of 1,680 women. 26 of the women, following cytological screening, were treated surgically with a loop electrosurgical excision procedure (LEEP). Cases were studied histologically and were genotyped from paraffin blocks by applying a commercial kit that recognized 35 HPV types. Cytological diagnoses included 17 HSIL, 1 LSIL, 5 ASC-H and 3 AGUS. Histological diagnosis resulted in 3 cases of microinvasive squamous cell carcinoma stage IA of FIGO, 9 CIN-3, 8 CIN-2, 2 CIN-1, 3 flat condylomas and mild dysplasia of the endocervical epithelium. Fifteen of twenty-five cases genotyped were positive for HPV (60%). HPV 16 and 33 were identified in four cases each, HPV 58 in two other cases, and HPV 18, 31, 52, and 82 in one case, with one HPV 16 and 58 coinfection. The frecuency of HPV types in the African area varies in comparison to other regions, particularly in Europe and USA. Vaccination against the five most common HPV types (16, 33, 58, 18, and 31) should be considered in the geographic region of West Africa and specifically in Equatorial Guinea.
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1.30Impact points
Losses of expression of the antigens A, Le(a) and Le(x) and over-expression of Le(y) in carcinomas and HG-SIL of the uterine cervix.
Diagnostic pathology. 10/2008; 3(1):38.
ABSTRACT: BACKGROUND: The glycosilation of a great number of molecules, glyco-protein or glycolipids, has been of interest for decades. OBJECTIVE: To compare the expressive patterns of the isoantigenic determinants of histo-blood groups ABH and Lewis in squamous and simple epithelium and in precurso... [more] ABSTRACT: BACKGROUND: The glycosilation of a great number of molecules, glyco-protein or glycolipids, has been of interest for decades. OBJECTIVE: To compare the expressive patterns of the isoantigenic determinants of histo-blood groups ABH and Lewis in squamous and simple epithelium and in precursors and cancers of the cervix. METHODS: A total of 36 lesions and neoplasms (10 LG-SIL, 16 HG-SIL and 10 invasive carcinomas) have been studied with immunohistochemical techniques, using monoclonal antibodies (MoAb BG1 to BG8) for precursor chains, blood-group ABH and Lewis group Le(a), Le(b), Le(x), and Le(y), and four types of lectins. In addition, we have studied the expression of p53 protein and PCNA, establishing the rate of proliferation of each lesion. Using PCR techniques, we have also detected part of the intron of the E6 gene of HPV-16. RESULTS: In the invasive cervical carcinomas, we observed a loss of expression of the Le(x) antigen (p<0.01). With regard to the progression of the different lesions studied, we found alterations in the patterns of expression of the antigens of the ABH and Lewis blood groups. There was a tendency towards a loss of expression and heterogeneous patterns in the more advanced lesions, as well as over-expression of the Le(y) antigens. With PCNA, we established a proliferative rate which tended to be greater in relation to the progression of the cervix neoplasms. CONCLUSION: These results indicate that there is a relation between the losses of histo-blood groups and the progression of the squamous intraepithelial lesions.
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1.30Impact points
GFAP and alpha1a-AR staining and nuclear morphometry of oligodendrogliomas by confocal microscopy and image analysis: useful parameters for predicting survival in oligodendrogliomas.
Diagnostic pathology. 02/2008; 3 Suppl 1:S26.
ABSTRACT : OBJECTIVE : This study attempts to evaluate the GFAP and alpha1a-AR staining and morphometrical nuclear features of oligodendrogliomas and their prognostic implications as compared to present histopathology classification and their survival outcome. STUDY DESIGN : Surgical specimens from ... [more] ABSTRACT : OBJECTIVE : This study attempts to evaluate the GFAP and alpha1a-AR staining and morphometrical nuclear features of oligodendrogliomas and their prognostic implications as compared to present histopathology classification and their survival outcome. STUDY DESIGN : Surgical specimens from 24 patients with oligodendrogliomas during the period 1981-2000 were included. These cases were classified into two groups defined by the grade of the neoplasm: Group I: oligodendrogliomas grade II; Group II: oligodendrogliomas grade III and two groups based on the outcome status: Group of the alive cases and group of the death cases. Death rate for the groups were obtained by patients' charts. Descriptive statistics were used to examine the groups with respect to the morphometrical nuclear variables; area, perimeter, aspect, axes (major and minor), diameters (max, mean and min.), radius (max. and min.) margination, ratio of perimeter-area, roundness and sizes (length and width). In addition, an immunofluorescence method for GFAP and 1a-AR were performed and their area, density and intensity of staining were analyzed. RESULTS : Semiautomated quantitative morphometrical results showed that the variables of nuclear area (GII 48.87 mum2 vs. GIII 43.45 mum2 p-value = 0.02), aspect (GII 1.39 vs. GIII 1.55 p-value = 0.03), axis minor (GII 6.66 mum vs. GIII 6.01 mum p-value = 0.003), diameter minor (GII 5.93 mum vs. GIII 5.27 mum p-value = 0.002), radius minor (GII 2.64 mum vs. GIII 2.25 mum p-value = 0,003), perimeter-area (GII 0.0007 vs. GIII 0.0006 p-value = 0.04), size width (GII 6.60 mum vs. GIII 5.96 mum p-value = 0,003), and density of alpha1a-AR staining (GII 121.38 vs. GIII 146.03 p-value = 0.05) were statistically significant in regard of grade; and that the sum of density of GFAP (p-value = 0.01) and the intensity of alpha1a-AR (p-value = 0.01) were statistically significant in predicting survival. CONCLUSION : These results suggest that some nuclear morphometrical features and the GFAP and alpha1a-AR immunofluorescence staining may be useful parameters for predicting survival in oligodendrogliomas.
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1.30Impact points
Discontinuous video recording of biopsies in the context of an integral third degree teaching program.
Diagnostic pathology. 02/2008; 3 Suppl 1:S8.
ABSTRACT : BACKGROUND : The application of Information and Communication Technologies (ICTs) in the field of university education can play an important role in order to fulfil the objectives of the European Space for Higher Education (ESHE). In the absence of an infrastructure that allows virtual pr... [more] ABSTRACT : BACKGROUND : The application of Information and Communication Technologies (ICTs) in the field of university education can play an important role in order to fulfil the objectives of the European Space for Higher Education (ESHE). In the absence of an infrastructure that allows virtual preparations, the high definition video may become an alternative for many PC users and telepathology enthusiasts. METHODS : We have begun a video biopsy discontinuous recording pilot programme, also known as interval cinematography, in order to give our students pathological images to gather documentary evidence to follow their clinical cases. We have a working post available with a ZEISS Axioshop 2 microscope connected to a JVC digital camera with a double output for video, a PVM_14N1MDE Sony Triniton colour monitor and a LG RH199 HDD-DVD recorder. RESULTS : Pathologists can easily produce videos lasting between 45" and 5' according to the case. Later those videos are assessed all together and given to the student and/or the doctor who requested the case study. CONCLUSION : Better follow-up studies of the cases can be achieved using Discontinuous video recording of biopsies. This approach has also clinical benefits since it contributes to the enrichment of integral patient care.
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4.43Impact points
Proteomic analysis of lung biopsies: Differential protein expression profile between peritumoral and tumoral tissue.
Proteomics. 03/2004; 4(2):442-7.
The cellular proteome shows a dynamic profile and is subjected to changes in response to various stimuli and disease progression. Lung cancer remains the leading cause of cancer death in industrialized countries. In an attempt to find new disease markers, patients suffering from lung carcinoma have ... [more] The cellular proteome shows a dynamic profile and is subjected to changes in response to various stimuli and disease progression. Lung cancer remains the leading cause of cancer death in industrialized countries. In an attempt to find new disease markers, patients suffering from lung carcinoma have been selected to achieve differential protein expression patterns between normal and neoplasic tissue. After two-dimensional electrophoresis, the spots of interest were digested and identified by matrix-assisted laser desorption/ionization (MALDI) peptide mass fingerprinting. This information will provide a more comprehensive understanding of the disease progression and might constitute a method to complement histopathological diagnosis.
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2.40Impact points
Sympathetic sprouting in dorsal root ganglia (DRG): a recent histological finding?
Histology and histopathology. 05/2003; 18(2):575-86.
During the nineties it was described, as an original finding, the existence of afferent amyelinic nerve endings in animal dorsal root ganglia (DRG) caused by diverse experimental lesions. These works do not take into account the historical studies carried out by Ehrlich (1886), Ramón y Cajal (1890) ... [more] During the nineties it was described, as an original finding, the existence of afferent amyelinic nerve endings in animal dorsal root ganglia (DRG) caused by diverse experimental lesions. These works do not take into account the historical studies carried out by Ehrlich (1886), Ramón y Cajal (1890) and Dogiel (1885) among others. Ramón y Cajal (1899) confirmed the existence of these nerve endings naming them after their discoverer as "Dogiel's arborisations". Ramón y Cajal claims that these endings originate from fibres of sympathetic nature, something supported by later authors devoted to this topic. In any case, the same authors remarked already a possible relationship with pathological phenomena, nonetheless always referring to the frequent occasions in which the same images appeared in healthy animals. In this work we review the bibliography about the classically named "Terminal Dogiel's nests" which in modern literature have been referred to as sprouting of sympathetic axons in dorsal root ganglia likely related with sympathetically maintained pain. Furthermore, we present the finding, not described up to date, of multiple afferent amyelinic nervous endings related with the "Terminal Dogiel's nests" observed in different DRG from young adult healthy rabbits.
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2.40Impact points
Histomorphometrical study of the submandibular gland ductal system in the rat.
Histology and histopathology. 02/2002; 17(3):813-6.
The duct system of murine submandibular gland is composed, in contrast with other mammals, by four types of ducts, among which the granular duct is unique for rodents. The granular duct shows a typical secretory structure with a clear intersex morphological diversity on which we carried out a morpho... [more] The duct system of murine submandibular gland is composed, in contrast with other mammals, by four types of ducts, among which the granular duct is unique for rodents. The granular duct shows a typical secretory structure with a clear intersex morphological diversity on which we carried out a morphometrical study in order to determine the relative area of each duct in rats in comparison with the rest of ducts and the whole gland. Our results, in both sexes, show that the duct with the broadest surface is the granular duct, followed by the excretory, striated and the intercalated ducts. In addition, we found a significant intersex difference between the relative surface of the granular and the excretory ducts, being bigger in males than in females. Finally, in both sexes, there is a greater variation in the data related to the excretory ducts than to the other ducts.
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2.02Impact points
Squamous cell carcinoma of the oral cavity: a clinicopathologic scoring system for evaluating risk of cervical lymph node metastasis.
The Laryngoscope. 07/1995; 105(7 Pt 1):728-33.
A series of 126 cases of squamous cell carcinoma of the oral cavity with neck dissection was studied. Several clinicohistologic parameters and statistical correlation with lymph node metastasis were evaluated. Statistical association with metastasis was found in the following factors: microvascular ... [more] A series of 126 cases of squamous cell carcinoma of the oral cavity with neck dissection was studied. Several clinicohistologic parameters and statistical correlation with lymph node metastasis were evaluated. Statistical association with metastasis was found in the following factors: microvascular invasion (P < 6 x 10(-7)), grade of differentiation (P < .0001), T category (P = .003), tumor thickness (P = .005), inflammatory infiltration (P = .011), tumoral interphase (P = .02), and perineural spread (P = .04). We designed a scoring system based on statistical results that eliminates the need for complex mathematical formulas. We tested this scoring system; cases with scores ranging form 7 to 12, 13 to 16, 17 to 20, and 21 to 30 points showed metastasis in 0%, 20%, 63.6%, and 86.3% of cases, respectively. The differences between these four groups in relation to rate of metastasis were very significant (P = 8 x 10(-9)). In conclusion, this scoring system is a reliable method of predicting risk of metastasis.
Following (50)
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Alberto Moran
Universidad Complutense de Madrid -
Myriam Catalá
Universidad Rey Juan Carlos -
Ijad Madisch
Massachusetts General Hospital -
Bárbara Abreu
cooperativa de ensino superior, politécnico e universitário -
Marcial Garcia-Rojo
Hospital General Universitario de Ciudad Real
Topics (12)
