Skills (22)
Research experience
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Sep 2009
Research: Function of intestinal intracellular lipid binding proteins
McGill University · Nutrition · McGill UniversityAgellon Lab · Montreal -
Sep 2007–
Jul 2009Research: The Effect of Wild blueberries on Satiety and glycemic control
University of Maine · Human Nutrition · University of MaineOronoWild blueberries, obesity, diabetes, satiety, glycemia, anthocyanins, flavanoids
Education
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Sep 2009
McGill University
Molecular Nutrition · PhDCanada · Montreal -
Sep 2007–
Aug 2009University Of Maine
Clinical Research/Dietetics · M.S. Human Nutrition and Food ScienceUnited States of America (USA) · Orono -
Jan 2006–
May 2007Johnson and Wales
Nutrition/Dietetics · Culinary NutritionUnited States of America (USA) · Providence
Questions and Answers (15) View all
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Answer added in Gel Electrophoresis20 Maximum resolution on agarose gelAll of the above recommendations not withstanding I found that one of the best ways to increase resolution of an agarose gel is to cast a thin gel. ... [more]
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Question asked in Computer SoftwareOpen Exporting data from Chemstation to ExcelI am trying to export data generated from several runs from chemstation to excel. From the research I have done this seems to be a pretty common probl... [more]
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Answer added in Fatty Acids4 Organic Lipid ExtractionIn this case, it is a modified B&D but my question could be applied to either method.
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Question asked in Fatty Acids4 Organic Lipid ExtractionOnce you remove the organic phase from your sample, do you aliquot a defined amount from all your samples and then dry it down and reconstitute or do ... [more]
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Answer added in Paper / Thin Layer Chromatography6 Does anyone have experience with the overnight charring of lipids separated by TLC?Currently, I am just working with what we have (and my adviser suggested it). I also visualized the bands by UV shadowing but a permanent record would... [more]
Publications (2) View all
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Article: The ileal lipid binding protein is required for efficient absorption and transport of bile acids in the distal portion of the murine small intestine.
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ABSTRACT: The ileal lipid binding protein (ilbp) is a cytoplasmic protein that binds bile acids with high affinity. However evidence demonstrating the role of this protein in bile acid transport and homeostasis is missing. We created a mouse strain lacking ilbp (Fabp6(-/-) mice) and assessed the impact of ilbp deficiency on bile acid homeostasis and transport in vivo. Elimination of ilbp increased fecal bile acid excretion (54.2%, P<0.05) in female but not male Fabp6(-/-) mice. The activity of cholesterol 7α-hydroxylase (cyp7a1), the rate-controlling enzyme of the classical bile acid biosynthetic pathway, was significantly increased in female (63.5%, P<0.05) but not in male Fabp6(-/-) mice. The amount of [(3)H]taurocholic acid (TCA) excreted by 24 h after oral administration was 102% (P<0.025) higher for female Fabp6(-/-) mice whereas it was 57.3% (P<0.01) lower for male Fabp6(-/-) mice, compared to wild-type mice. The retained fraction of the [(3)H]TCA localized in the small and large intestines was increased by 22% (P<0.02) and decreased by 62.7% (P<0.01), respectively, in male Fabp6(-/-) mice relative wild-type mice, whereas no changes were seen in female Fabp6(-/-) mice. Mucosal to serosal bile acid transport using everted distal gut sacs was decreased by 74% (P<0.03) in both sexes of Fabp6(-/-) mice as compared to wild-type mice. The results demonstrate that ilbp is involved in the apical to basolateral transport of bile acids in ileal enterocytes, and is vital for the maintenance of bile acid homeostasis in the enterohepatic circulation (EHC) in mice.PLoS ONE 01/2012; 7(12):e50810. · 4.09 Impact Factor -
Article: The Effects of Wild-Blueberries on Satiety and Glycemic Control
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ABSTRACT: The prevalence of type 2 diabetes and obesity is increasing in the United States and other nations. Highly digestible carbohydrates may promote a high glycemic response, possibly contributing to obesity-related diseases. Anthocyanins have been found to exert in vitro α-glucosidase inhibitory effect, suggesting that foods containing anthocyanins may improve glycemic control. Wild Maine lowbush blueberries, Vaccinium angustifolium Ait., are a rich source of anthocyanins and contain 6 grams of dietary fiber and 45 kcal per 140 grams. The objective of this study was to evaluate the effect of wild blueberries and their juice on post-prandial serum glucose and satiety. A randomized cross-over blinded study was conducted using 11 overweight (body mass index (BMI) 25-29.9 kg/m2) and 10 normal weight (BMI 18.5-24.9 kg/m2) subjects who were 25-50 years old. Subjects were provided a base meal of cornflakes, milk and orange juice after an overnight fast. Four meal types were tested. One treatment included one cup (140g) of lowbush wild Maine blueberries; another had 112 mL of 100% wild blueberry juice. A placebo beverage mimicked the equivalent volume, acidity, and sugars to that of blueberry juice, was the third treatment, and lastly, a control meal with added glucose and fructose to match the amount in the berry meal. All meals as well as the control were adjusted to provide the same amount of carbohydrates, simple sugars, and calories. Fasting serum triglycerides and glucose were measured at baseline, and at 30, 60, 90, and 120 minutes. Serum insulin was measured at baseline, 30 and 60 minutes. Serum triglycerides and glucose was evaluated with the Beckman clinical analyzer, while serum insulin was determined with a FLUOstar Omega plate reader. Satiety was measured utilizing a visual analog scale (VAS) at baseline, 15, 30, 45, 60, 90, 120, and 180 minutes. After each intervention, participants kept food journals for the remainder of the day. Area under the curve was evaluated for overall changes in satiety responses. Results were analyzed using SYSTAT analytical software. A repeated measure General Linear Model was utilized for analysis of control and treatment groups. Test meals had no effect on serum glucose levels, insulin, triglycerides, or energy intake. Satiety responses differed among subjects with overweight and normal BMIs. Overweight subjects were more satisfied (P=0.05) and full (P=0.002) when compared to their lower BMI counterparts throughout all treatments. More human research is needed in order to evaluate the mechanisms by which anthocyanins may affect glycemic control, as well as to determine optimal dose efficacy.University of Maine. 01/2009;
