El Mahdy Mohamed El Mahdy

Ph.D student
National Research Centre · Water pollution Research Department, Environmental Virus Lab.

I love Biological Science

Research skills

  • Technical
    1- Apoptotic behaviour of different cell lines infected with viruses, 2- PCR, nested PCR, Semi-Nested PCR, Real time PCR (one-step and two step) ELISA, Antiviral assay, cytotoxicity test, Extraction of DNA and RNA using BioZol, Sequencing, quantification using internal control, Plaque Assay, and using different cell lines for viral isolation, QIAGEN
  • IT
    Excel, Power point, Movie Maker, Photoshop

Research interests

  • Interests
    Molecular Virology, Environmental and Molecular Virology, Water-borne and Food-borne viruses, Enteric Hepatitis Viruses(HAV, HEV) and other Enteric Viruses like Adenoviruses and Rotaviruses cause they are most frequent in Egypt and its zoonotic relationship, 1- Determination of the possible mechanisms of apoptosis of different cell lines infected with enteric viruses. 2- Definition the natural products that may play a role in the enhancement of programmed cell death and/or cellular protection against enteric

Research experience

  • Teaching: I am teaching some external researchers and students on PCR technology and other molecular Techniques (practical and oral) in the water pollution department training courses which are held every year.
  • Dec 2010–
    Dec 2013
    Research: Host and viral factors in acute hepatitis C {HEALTH. 2010-1.2.4 Early events in acute hepatitis C virus (HCV) infection with the aim to identify new biomarkers (2010-2013), ACRONYM: HepaCute}. Funded by European commission [FP-7 projects-EU].
    National Research Centre · FP-7 projects-EU · National Research Centre
  • Jan 2010–
    Dec 2012
    Research: 8. Phytochemical screening and bioactivity of some freshwater algae on hepatitis C virus (HCV). Funded by Science and Technology Development Fund (STDF-Egypt).
    National Research Center (NRC) · Center Of Excellence for Advanced Science, Virology Lab. · National Research Center (NRC)
    Cairo
    HCV, Antiviral, Algal extract

Other

  • Languages
    Arabic,English
  • Other Interests
    football,Reading ,and walking, Applied and environmental Microbiology, Journal of Medical Virology, Science direct,Water Research Journal., 1. El-Senousy, W.M., and El-Mahdy, M. El-Mahdy, (2008). Detection
    and Genotyping of Rotavirus Genogroup A in Water Treatment Plants in
    El-Dakahlia Governorate. Egyptian Journal of Biotechnology.
    2. El-Senousy, W.M., Costafreda, M., El-Mahdy, M. El-M., Nasr,
    M., Pinto, R.M., and Bosch. A. (2009). Development of Real time
    RT-PCR for quantification of hepatitis viruses in clinical and
    environmental samples. (Paper presented in the first International
    Conference of Clinical and Environmental Virology on
    Development of analytical methods for detection and quantification
    of viruses. (Instituto Superior Tecnico, Lisbon, Portugal 6-7
    October).
    3. El-Mahdy, M. El-Mahdy; Waled M. El Senousy and Mohamed N
    Fathi. (2010). Detection and genotyping of Rotavirus group A in two
    different types of drinking water treatment facilities. Paper presented in
    the 4th International Conference of “Environmental Science and
    Technology, Egypt, 2010” December 14-16.
    4- Waled M. El Senousy; Mohamed N. Fathi and El-Mahdy, M. El-
    Mahdy. (2010). Development of real time RT-PCR for quantification of
    noroviruses in agriculture products and irrigation water. Paper presented
    in the 4th International Conference of “Environmental Science and
    Technology, Egypt, 2010” December 14-16.
    5- Waled M. El Senousy; Mohamed N. Fathi and El-Mahdy, M. El-
    Mahdy. (2010). Chlorine inactivation of noroviruses, rotaviruses, and
    HEV in drinking water. Paper presented in the 4th International
    Conference of “Environmental Science and Technology, Egypt, 2010”
    December 14-16.
    6. El-Mahdy, M. El-Mahdy; Waled, M. El-Senousy; Gamila, H. Ali and
    Mohamed, M. Kamel. (2011). Role of Domestic Animal and Birds in the
    Contamination Of Water Resources With Zoonotic Viruses. Manuscript
    under preparation in International Journal of Water Research., Hepatitis viruses, Waterborne Zoonosis and Foodborne viruses

Publications

  • Role of domestic animals and bird in the contamination of water resources with zoonotic viruses

    Waled M el senousy, El mahdy M. El Mahdy

    01/2010

    Degree: Master degree

    Supervisor: Prof. Dr. Waled Morcy EL senousy

  • Role Of Domestic Animals An Birds In the Contamination Of Water Recources with Zoonotic Viruses.

    Waled M.El-Senousy, El-Mahdy M.El-Mahdy

    Journal of Egyptian Virology. 10/2009;

  • Detection and Genotyping of Rotavirus Genogroup A in Water Treatment Plants in El-Dakahlia Governorate

    Waled M. El-Senousy, El-Mahdy M.El-Mahdy

    Journal Of Egyptian Biotechnology. 01/2009;

    The presence of rotavirus strains in water samples was investigated by using a generic molecular detection method based on amplification of a VP6 gene fragment. The samples were collected from different treatment steps of traditional water treatment plant (WTP) (Meet Khamees) and two compact units (... [more] The presence of rotavirus strains in water samples was investigated by using a generic molecular detection method based on amplification of a VP6 gene fragment. The samples were collected from different treatment steps of traditional water treatment plant (WTP) (Meet Khamees) and two compact units (Shoha and Mahalet Damana) from El-Dakahlia Governorate, Egypt (October 2006 to September 2007) Overall, the percentage of rotavirus positive samples was 50%, 33.3%, and 25% in raw water of Meet Khamees, Shoha, and Mahalet Damana, respectively. Only one time succession of viral removal was observed in both Meet Khamees and Mahalet Damana WTPs while complete failure in viral removal was observed in Shoha WTP. Positive samples were characterized further, and VP7 and VP4 genotypes were determined. Although 15.2% of the positive samples were G untypeable, the distribution of G types in the positive samples was 75% G1, 25% G3. When the P types were examined, 9.3% of the positive samples were untypeable, and the distribution of types in the positive samples was 56.7% P[4], 33.3% P[8], and 10% P[6]. The distribution for strains of rotaviruses was 39.3% P[4]G1, 28.6% P[8]G1, 17.6% P[4]G3, 7.1% P[8]G3, 7.1% P[6]G1.

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