University of TorontoBiomedical Engineering · Doctor of PhilosophyCanada · Toronto
University of MichiganMolecular, Cellular, and Developmental Biology · Masters of ScienceUSA · Ann Arbor
University of MichiganBiology · Bachelors of ScienceUSA · Ann Arbor
Article: Use of fresh osteochondral glenoid allograft to treat posteroinferior bone loss in chronic posterior shoulder instability.[show abstract] [hide abstract]
ABSTRACT: We report our experience with the use of fresh glenoid osteochondral allograft in the treatment of a chronic posttraumatic posterior subluxation of the shoulder associated with glenoid bone loss in a 54-year-old recreational football player. Based on the pathoanatomy of the lesion and availability of a bone bank providing fresh allograft, we opted for an open anatomic reconstruction using a fresh glenoid allograft. A posterior approach was used; the prepared allograft was placed in the appropriate anatomic position and fixed with 2 small fragment screws with washers. At 2-year follow-up, the clinical outcome is excellent. This procedure may represent an effective option for the treatment of chronic posterior shoulder instability due to glenoid bone loss. However, the long-term efficacy and the progression of glenohumeral osteoarthritis need to be evaluated.American journal of orthopedics (Belle Mead, N.J.) 02/2013; 42(2):78-82.
[show abstract] [hide abstract]
ABSTRACT: Preserving the ability to maintain an active lifestyle is a major concern in the reconstruction of the knee in young patients. For the healthy individual who desires to maintain a relatively active lifestyle, fresh osteochondral allografts may serve as an alternative to total joint reconstruction. The use of fresh allografts is primarily indicated in the patient suffering from a traumatic loss of articular segments, who is too young or active for arthroplasty. In addition, fresh osteochondral allografts have a number of advantages over arthroplasty such as providing surgeons with a source of large grafts that can be fitted to replace osteochondral defects and cover the majority or entirety of articular surfaces without any donor site morbidity. In this case, a young, active patient lost a 7 x 8 cm portion of their distal femur, including a large portion of the articulating surface. Using a fresh osteochondral allograft, harvested within 24 hours of donor death, a segment was fitted to match bony apposition, articular congruity, and congruity with the femoral notch and affixed with four partially threaded cancellous screws. Joint function was restored with the allograft in place, allowing the patient to delay the need for a total joint replacement.McGill journal of medicine: MJM: an international forum for the advancement of medical sciences by students 06/2011; 13(1):22.
[show abstract] [hide abstract]
ABSTRACT: Hip osteoarthritis is prevalent in 8% to 28% of patients with Down syndrome. Presence of disabling hip pain is increased along with prolonged life expectancy, suggesting total hip arthroplasty (THA). Seven consecutive patients (9 hips) with Down syndrome underwent primary THA. Coxarthrosis was secondary to developmental hip dysplasia in 6 patients and slipped capital epiphysis in 1 patient. In 5 patients (7 hips), a previous hip surgery was performed. Average clinical and radiological follow-up was 9.9±6.4 years (range, 2-22.5 years; median, 9.3 years). Average patient age at THA was 34.8±7.5 years (range, 25- 47 years; median, 35.4 years). In 2 patients (3 hips), a trochanteric slide was used for the surgical approach, while a lateral transgluteal approach was used in the remaining patients. One-way analysis of variance test was used to compare Harris Hip Scores (HHS) at postoperative follow-up.Harris Hip Scores improved significantly (P=.008) from 4.1±15.1 (range, 18.5-65; median, 45) to 84.8.3±7.7 (range, 70-93; median, 85.8) at 4-year follow-up. Harris Hip Scores (average, 70.9±6.2; range, 66.5-80; median, 68) remained essentially unchanged (P=.43) at 8-year follow-up. Two patients required revision THA for stem loosening at 6 and 16 years post-THA, respectively. The first patient is 7 years post-revision and ambulates without aids. The second patient is 6.1 years post-revision and ambulates with a walker. Six of the THAs required a constrained liner. No dislocations or deep infections were encountered. At last follow-up, all patients had a functional range of motion without evidence of discomfort related to their THA.Orthopedics 09/2010; 33(9):629. · 2.66 Impact Factor
Yona Kosashvili, Drew Taylor, David Backstein, Oleg Safir, Boaz Liberman, Dror Lakstein, Allan Gross[show abstract] [hide abstract]
ABSTRACT: Patients with Down's syndrome (DS) have an increased incidence of coxarthrosis which may become symptomatic with prolonged life expectancy. We present seven consecutive patients (nine hips) with DS who had primary total hip arthroplasty (THA). Average clinical and radiological follow-up was 9.9 ± 6.4 years (range 2-22.25). Harris hip scores (HHS) improved significantly (p < 0.01) from 41.1 (range 18.5-65) to 80.2 (range 67.5-91) at latest follow-up. Two patients required revision arthroplasty for stem loosening at 16 (osteolysis) and six years (trauma) following THA, respectively. Six of the THAs required a constrained liner. No dislocations or deep infections were encountered. We contend that THA is a reliable surgical intervention in patients with DS and may be performed in symptomatic patients.International Orthopaedics 05/2010; 35(5):661-6. · 2.03 Impact Factor
Article: Hyaline cartilage tissue is formed through the co-culture of passaged human chondrocytes and primary bovine chondrocytes.Drew W Taylor, Nazish Ahmed, Anthony J Hayes, Peter Ferguson, Allan E Gross, Bruce Caterson, Rita A Kandel[show abstract] [hide abstract]
ABSTRACT: To circumvent the problem of a sufficient number of cells for cartilage engineering, the authors previously developed a two-stage culture system to redifferentiate monolayer culture-expanded dedifferentiated human articular chondrocytes by co-culture with primary bovine chondrocytes (bP0). The aim of this study was to analyze the composition of the cartilage tissue formed in stage 1 and compare it with bP0 grown alone to determine the optimal length of the co-culture stage of the system. Biochemical data show that extracellular matrix accumulation was evident after 2 weeks of co-culture, which was 1 week behind the bP0 control culture. By 3 to 4 weeks, the amounts of accumulated proteoglycans and collagens were comparable. Expression of chondrogenic genes, Sox 9, aggrecan, and collagen type II, was also at similar levels by week 3 of culture. Immunohistochemical staining of both co-culture and control tissues showed accumulation of type II collagen, aggrecan, biglycan, decorin, and chondroitin sulfate in appropriate zonal distributions. These data indicate that co-cultured cells form cartilaginous tissue that starts to resemble that formed by bP0 after 3 weeks, suggesting that the optimal time to terminate the co-culture stage, isolate the now redifferentiated cells, and start stage 2 is just after 3 weeks.Journal of Histochemistry and Cytochemistry 05/2012; 60(8):576-87. · 2.72 Impact Factor