Dr Virendra Gomase

Publications

• ANTIGEN PROTEIN FROM XYLELLA FASTIDIOSA: NEW PARADIGM OF SYNTHETIC VACCINE DEVELOPMENT

  V.S.* Gomase

  International Journal of Pharmacy and Technology. 01/2010;

  Xylella fastidiosa is a pathogenic bacterium that infects plants, causing a variety of diseases in over 100 plant species. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in pathoge... [more] Xylella fastidiosa is a pathogenic bacterium that infects plants, causing a variety of diseases in over 100 plant species. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in pathogenic diseases. Analysis shows MHC class II binding peptides of antigen protein from Xylella fastidiosa are important determinant for protection of host form infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 790 amino acids, which shows 782 nonamers.Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & IImolecules is important in vaccine development from Xylella fastidiosa.

• Isolation, structure elucidation and biotransformation studies on secondary metabolites from Asparagus racemosus

  V.S. Gomase, A.S. Sherkhane

  International Journal of Microbiology Research. 01/2010;

  Asparagus racemosus is important medicinal plant of tropical and subtropical India, is apotent phytoestrogen, which is used extensively in the treatment of menopausal symptoms, diarrhea,dyspepsia, and neurodegenerative disorders. The multiple uses of this species have increased itscommercial demand ... [more] Asparagus racemosus is important medicinal plant of tropical and subtropical India, is apotent phytoestrogen, which is used extensively in the treatment of menopausal symptoms, diarrhea,dyspepsia, and neurodegenerative disorders. The multiple uses of this species have increased itscommercial demand resulting in over-exploitation. Consequently, the plant is recognized as being"critically endangered" in its natural habitat. Development of an efficient Isolation, structure elucidationand biotransformation studies protocol will not only play a significant role in meeting the requirement ofplanting material for commercial cultivation, but also in aiding the conservation process.

• Study of protein metabolism of Gallus gallus domesticus (Linn, 1758) infected by Raillietina (Eucestoda)

  S.B. Waghmare, V.S. Gomase, R.J. Chavan

  International Journal of Parasitology Research. 01/2010;

  The values and dynamics of some indices of protein metabolism were studied in domestic birdGallus gallus domesticus infected with cestodes Railletina echinobothrida, (Magnin, 1881) in natural populationand in experiment. These indices were compared in infected and non- infected birds. Cestode infect... [more] The values and dynamics of some indices of protein metabolism were studied in domestic birdGallus gallus domesticus infected with cestodes Railletina echinobothrida, (Magnin, 1881) in natural populationand in experiment. These indices were compared in infected and non- infected birds. Cestode infectionconsiderably affected hosts protein metabolism irrespective of the age; however the chances were morepronounced in younger ones. Increased concentration of -globulin, modified albumin and circulating immunecomplexes was observed in plasma of infected host. The experiment demonstrated the most significantchanges in the protein metabolism of Gallus gallus domesticus 4- 8 days after infestation with cestodesRailletina echinobothrida (Magnin, 1881).

• Microbial proteomics approach for synthetic vaccine development from Taenia solium

  Gomase VS And Chitlange NR

  International Research Journal of Biotechnology, http://www.interesjournals.org/IRJ0B. 01/2010; 1:023-027.

  Taenia solium, also called the pork tapeworm causes cysticercosis in humans. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in parasitic diseases. Analysis shows MHC class II bindi... [more] Taenia solium, also called the pork tapeworm causes cysticercosis in humans. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in parasitic diseases. Analysis shows MHC class II binding peptides of antigen protein from Taenia solium are important determinant for protection of host form parasitic infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 389 amino acids, which shows 381 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Taenia solium

• Machine Intelligence Approach for Development of MHC Binders and Fragment Based Peptide Vaccines from Tetrahymena pyriformis

  Gomase VS, Chitlange NR

  Global Journal of Biotechnology. 01/2010; 5:161-165.

  Tetrahymena pyriformis are free-living ciliate protozoa that Guppies are easily susceptible to Tetrahymena. Peptide fragments of protein antigen can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in parasitic diseases. An... [more] Tetrahymena pyriformis are free-living ciliate protozoa that Guppies are easily susceptible to Tetrahymena. Peptide fragments of protein antigen can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in parasitic diseases. Analysis shows MHC class II binding peptides of protein antigen from Tetrahymena pyriformis are important determinant for protection of host form parasitic infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of protein antigen having 389 amino acids, which shows 381 nonamers. Binding ability prediction of antigenic peptides to major histocompatibility complex (MHC) class I and II molecules is important in vaccine development from Tetrahymena pyriformis.

• Exploration of Schizophrenia: A neurological disorder using bioinformatics tools and techniques

  Bajaj NJ, Chitlange NR, Shaik Ubed, Sanjaykumar Chaubey, Gomase VS

  International Journal of Computational Biology, http://www.bioinfo.in/journalcontent.php?id=220. 01/2010; 1:01-03.

  Schizophrenia is one of the most confusing and disabling mental illness that makes it difficult to tell the difference between real and unreal experiences, to think logically, to have normal emotional responses, and to behave normally in social situations. Schizophrenia may occur due to environmenta... [more] Schizophrenia is one of the most confusing and disabling mental illness that makes it difficult to tell the difference between real and unreal experiences, to think logically, to have normal emotional responses, and to behave normally in social situations. Schizophrenia may occur due to environmental as well as the genetic factor. COMT is one of the genes responsible for Schizophrenia. We predicted the active site of COMT gene and its complementary ligand structures. This complementary ligand binds to the active site of the gene COMT. Binding ability of ligand to the COMT is important in drug design for curing Schizophrenia.

• Eimeria acervulina analysis for binding peptides using protein profiling for target validation

  Gomase VS, Kapoor RA, Ladak SS

  International Journal of Machine Intelligence, http://www.bioinfo.in/journalcontent.php?id=214. 01/2010; 2:01-08.

  Eimeria acervulina is a species of Eimeria that causes coccidiosis in older poultry. Lesions are limited to anterior or first third of the small intestine. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of role... [more] Eimeria acervulina is a species of Eimeria that causes coccidiosis in older poultry. Lesions are limited to anterior or first third of the small intestine. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious diseases. Analysis shows MHC class II binding peptides of antigen protein from Eimeria acervulina are important determinant for protection of host form parasitic infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 299 amino acids, which shows 291 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Eimeria acervulina

• Computer intelligence approach for prediction of binding ability and fragment based peptide vaccines from Leishmania protozoa peptides

  Gomase VS, Kapoor RA, Ladak SS

  2010 International Conference on Software and Computing Technology (ICSCT 2010), http://www.iacsit.org/proceeding/icsct10.toc.pdf; 01/2010

  Leishmania major, member of the genus Leishmania causes Leishmaniasis. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in protozoan diseases. Analysis shows MHC class II binding pep... [more] Leishmania major, member of the genus Leishmania causes Leishmaniasis. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in protozoan diseases. Analysis shows MHC class II binding peptides of antigen protein from Leishmania major are important determinant for protection of host form parasitic infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 389 amino acids, which shows 381 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Leishmania major

• Immunoproteomics approach for development of MHC binders and fragment based peptide vaccines from Tetrahymena pyriformis

  Gomase VS, Kapoor RA, Ladak SS

  Journal of Infectious Diseases Letters, http://www.bioinfo.in/uploadfiles/12841054611_1_2_JIDL.pdf. 01/2010; 1:07-11.

  Tetrahymena pyriformis are free-living ciliate protozoa that Guppies are easily susceptible to Tetrahymena. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in parasitic diseases. An... [more] Tetrahymena pyriformis are free-living ciliate protozoa that Guppies are easily susceptible to Tetrahymena. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in parasitic diseases. Analysis shows MHC class II binding peptides of antigen protein from Tetrahymena pyriformis are important determinant for protection of host form parasitic infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 389 amino acids, which shows 381 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Tetrahymena pyriformis.

• Binding ability prediction of antigen peptides to major histocompatibility complex for development of synthetic peptide vaccine from Plasmodium falciparum

  Gomase VS, Chitlange NR

  International Journal of Immunology Research, http://www.bioinfo.in/uploadfiles/12767045641_1_1_IJIR.pdf. 01/2010; 1:01-06.

  Plasmodium falciparum is causative agent of malaria. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious diseases. Analysis shows MHC class II binding peptides of antigen ... [more] Plasmodium falciparum is causative agent of malaria. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious diseases. Analysis shows MHC class II binding peptides of antigen protein from Plasmodium falciparum are important determinant for protection of host form infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 224 amino acids, which shows 216 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Plasmodium falciparum

• RNAi: Gene Silencing Approach in C. elegans and H. sapiens

  Gomase VS, More RP

  International Journal of Molecular Biology, http://www.bioinfo.in/uploadfiles/12680296361_1_3_IJMB.pdf. 01/2010; 1:09-14.

  siRNAs (Short interfering RNAs) and miRNAs (microRNAs), which are mediate silencing via distinct mechanisms C. elegans and Homo sapiens. The breaking of a doublestranded RNA (dsRNA) matching a specific gene sequence into short pieces called short interfering RNA, which trigger the degradation of mRN... [more] siRNAs (Short interfering RNAs) and miRNAs (microRNAs), which are mediate silencing via distinct mechanisms C. elegans and Homo sapiens. The breaking of a doublestranded RNA (dsRNA) matching a specific gene sequence into short pieces called short interfering RNA, which trigger the degradation of mRNA that matches its sequence. In this review, we discussed the RNA interference with its principle and application in recent biological research areas.

• Support Vector Machine (SVM) based prediction of promiscuous MHC class binding peptides

  Gomase VS, Chitlange NR

  International Journal of Computational Biology, http://www.bioinfo.in/journalcontent.php?id=220. 01/2010; 1:04-08.

  Shigella flexneri is a species of Gram-negative bacteria in the genus Shigella that can cause diarrhea in humans. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious disea... [more] Shigella flexneri is a species of Gram-negative bacteria in the genus Shigella that can cause diarrhea in humans. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious diseases. Analysis shows MHC class II binding peptides of antigen protein from Shigella flexneri are important determinant for protection of host form parasitic infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 574 amino acids, which shows 566 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Shigella flexneri. Keywords- antigen protein, epitope, PSSM, SVM, MHC, peptide vaccine

• Application computational intelligence for development of synthetic peptide vaccine from Mycobacterium avium

  Gomase VS, Chitlange NR

  International Journal of Computational Biology, http://www.bioinfo.in/journalcontent.php?id=220. 01/2010; 1:09-13.

  Mycobacterium avium causes MAC diseases. Symptoms of MAC diseases are reminiscent of tuberculosis; they include fever, fatigue, and weight loss. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the im... [more] Mycobacterium avium causes MAC diseases. Symptoms of MAC diseases are reminiscent of tuberculosis; they include fever, fatigue, and weight loss. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious diseases. Analysis shows MHC class II binding peptides of antigen protein from Mycobacterium avium are important determinant for protection of host form bacterial infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 195 amino acids, which shows 187 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Mycobacterium avium.

• Microbial Proteomics Approach for Synthetic Vaccine Development form Bacillus Weihenstephanensis

  Gomase VS, Chitlange NR

  International Journal of Drug Design and Discovery, ijdddonline.com/issues/310_ab.pdf. 01/2010; 1:310-313.

  Bacillus weihenstephanensis causes systemic infections leading to be an obligatory step in infection. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious diseases. Analysi... [more] Bacillus weihenstephanensis causes systemic infections leading to be an obligatory step in infection. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious diseases. Analysis shows MHC class II binding peptides of antigen protein from Bacillus weihenstephanensis are important determinant for protection of host form bacteral infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of bacterial protein having 210 amino acids, which shows 202 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Bacillus weihenstephanensis.

• Immuno-proteomics approach for synthetic vaccine development form Haemophilus influenzae

  Gomase VS, Kapoor RA, Ladak SS

  Journal of Infectious Diseases Letters, http://www.bioinfo.in/uploadfiles/12841054921_1_1_JIDL.pdf. 01/2010; 1:01-06.

  Haemophilus influenzae causes systemic infections leading to be an obligatory step in infection. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious diseases. Analysis sho... [more] Haemophilus influenzae causes systemic infections leading to be an obligatory step in infection. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious diseases. Analysis shows MHC class II binding peptides of antigen protein from Haemophilus influenzae are important determinant for protection of host form pathogenic infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 575 amino acids, which shows 567 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Haemophilus influenzae

• Immunoproteomics approach for development of MHC binders and fragment based peptide vaccines from Onchocerca volvulus

  Gomase VS, Chitlange NR

  Drug Invention Today, http://ditonline.info/article/view/2828/1500. 01/2010; 2:297-299.

  Onchocerca volvulus is a nematode that causes Onchocerciasis or ‘River Blindness’. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious diseases. Analysis shows MHC class I... [more] Onchocerca volvulus is a nematode that causes Onchocerciasis or ‘River Blindness’. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious diseases. Analysis shows MHC class II binding peptides of antigen protein from Streptococcus dysgalactiae are important determinant for protection of host form nematode infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of bacterial protein having 147 amino acids, which shows 139 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Onchocerca volvulus.

• Immunoproteomics approach for development of MHC binders and fragment based peptide vaccines from Treponema pallidum

  Gomase VS, Chitlange NR

  Journal of Bioscience and Technology, http://www.jbstonline.com/documents/vol1issue2/jbst2010010204.pdf. 01/2010; 1:84-89.

  Treponema pallidum causes treponemal disease syphilis. Peptide fragments of antigen can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in bacterial diseases. Analysis shows MHC class II binding peptides of antigen from Tr... [more] Treponema pallidum causes treponemal disease syphilis. Peptide fragments of antigen can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in bacterial diseases. Analysis shows MHC class II binding peptides of antigen from Treponema pallidum are important determinant for protection of host form treponemal infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 443 amino acids, which shows 435 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Treponema pallidum antigen.

• Antigen protein from Schistosoma mansoni: new paradigm of synthetic vaccine development

  Gomase VS, Chitlange NR

  International Journal of Pharma and Bio Sciences, http://www.ijpbs.net/issue-3/44.pdf. 01/2010; 1:01-07.

  Schistosoma mansoni causative agent of schistosomiasis. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in parasitic diseases. Analysis shows MHC class II binding peptides of antige... [more] Schistosoma mansoni causative agent of schistosomiasis. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in parasitic diseases. Analysis shows MHC class II binding peptides of antigen protein from Schistosoma mansoni are important determinant for protection of host form parasitic infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 393 amino acids, which shows 385 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Schistosoma mansoni

• Role of MHC restriction in allogeneic immune responses to cancer

  Gomase VS, Parundekar AN

  International Journal of Genomics and Proteomics, http://www.bioinfo.in/uploadfiles/12767060741_1_3_IJGP.pdf. 01/2010; 1:12-24.

• An insight on the antigenic preparation strategies involved in the production of polyclonal antibodies against oligodendrogliomas (cerebral tumors)

  Kapoor RA, Ladak SS, Gomase VS

  Journal of Clinical Research Letters, http://www.bioinfo.in/uploadfiles/12801116161_1_2_JCRL.pdf. 01/2010; 1:11-17.

  Various strategies have been used to identify and characterize the antigens associated with human brain tumors. These approaches have included the raising of polyclonal and antibodies against tumor antigens and, more recently, efforts toward the direct biochemical identification of such proteins. Th... [more] Various strategies have been used to identify and characterize the antigens associated with human brain tumors. These approaches have included the raising of polyclonal and antibodies against tumor antigens and, more recently, efforts toward the direct biochemical identification of such proteins. This article summarizes the progress made in this area, suggests reasons for the broad antigenic cross-reactivity and heterogeneity revealed by these studies, and proposes additional methods such as radial and double immunodiffusion and ELISA for deciphering the complex antigenic composition of human brain tumors.