Dimitrije Stamenović

Publications (98) View all

• Source

  Available from: Dimitrije Stamenović

  Chapter: Comparative genomic approaches and technologies

  C L Smith, H Oh, D Stamenovic
  09/2012; , ISBN: 9780470027318
• Source

  Available from: Dimitrije Stamenović

  Article: A mathematical model of arrhythmogenesis in ventricular cardiomyopathies due to gap junction restructuring

  A P Pirentis, D Stamenovic
  Journal of Serbian Society of Computational Mechanics. 01/2012; 6:108-128.
• Source

  Available from: Dimitrije Stamenović

  Article: A micropatterning and image processing approach to simplify measurement of cellular traction forces.

  Samuel R Polio, Katheryn E Rothenberg, Dimitrije Stamenović, Michael L Smith
  [show abstract] [hide abstract]
  ABSTRACT: Quantification of the traction forces that cells apply to their surroundings has been critical to the advancement of our understanding of cancer, development and basic cell biology. This field was made possible through the development of engineered cell culture systems that permit optical measurement of cell-mediated displacements and computational algorithms that allow conversion of these displacements into stresses and forces. Here, we present a novel advancement of traction force microscopy on polyacrylamide (PAA) gels that addresses limitations of existing technologies. Through an indirect patterning technique, we generated PAA gels with fluorescent 1 μm dot markers in a regularized array. This improves existing traction measurements since (i) multiple fields of view can be measured in one experiment without the need for cell removal; (ii) traction vectors are modeled as discrete point forces, and not as a continuous field, using an extremely simple computational algorithm that we have made available online; and (iii) the pattern transfer technique is amenable to any of the published techniques for producing patterns on glass. In the future, this technique will be used for measuring traction forces on complex patterns with multiple, spatially distinct ligands in systems for applying strain to the substrate, and in sandwich cultures that generate quasi-three-dimensional environments for cells.
  Acta biomaterialia 08/2011; 8(1):82-8. · 3.98 Impact Factor
• Source

  Available from: Dimitrije Stamenović

  Article: A Model for Stress Fiber Realignment Caused by Cytoskeletal Fluidization During Cyclic Stretching.

  Athanassios P Pirentis, Elizabeth Peruski, Andreea L Iordan, Dimitrije Stamenović
  [show abstract] [hide abstract]
  ABSTRACT: Uniaxial cyclic substrate stretching results in a concerted change of cytoskeletal organization such that stress fibers (SFs) realign away from the direction of stretching. Recent experiments revealed that brief transient stretch promptly ablates cellular contractile stress by means of cytoskeletal fluidization, followed by a slow stress recovery by means of resolidification. This, in turn, suggests that fluidization, resolidification and SF realignment may be linked together during stretching. We propose a mathematical model that simulates the effects of fluidization and resolidification on cytoskeletal contractile stress in order to investigate how these phenomena affect cytoskeletal realignment in response to pure uniaxial stretching of the substrate. The model comprises of individual elastic SFs anchored at the endpoints to an elastic substrate. Employing the global stability convention, the model predicts that in response to repeated stretch-unstretch cycles, SFs tend to realign in the direction perpendicular to stretching, consistent with data from the literature. The model is used to develop a computational scheme for predicting changes in cell orientation and polarity during stretching and how they relate to the underlying alterations in the cytoskeletal organization. We conclude that depletion of cytoskeletal contractile stress by means of fluidization and subsequent stress recovery by means of resolidification may play a key role in reorganization of cytoskeletal SFs in response to uniaxial stretching of the substrate.
  Cellular and Molecular Bioengineering 03/2011; 4(1):67-80. · 1.95 Impact Factor
• Chapter: Cytoskeletal prestress as a determinant of deformability and rheology of adherent cells

  D Stamenovic
  01/2010: pages 92-118; , ISBN: 9878674663592