Devrim Demir Dora

Questions and Answers (3) View all

• Answer added in Molecular Genetics
  15 Mini-prep trouble!
  By Simone Newton-Payne · University of East Anglia
  Devrim Demir Dora · University of Illinois, Urbana-Champaign

  If you are sure that your ligation is done, take a single colony containing recombinant plasmid and culture it again on LB agar plates containing anti... [more]

  If you are sure that your ligation is done, take a single colony containing recombinant plasmid and culture it again on LB agar plates containing antibiotic which plasmid is resistant. Some times more than 24 hours is needed for incubating them at 37C. May be you have DNase contamination related with the solutions for alkali lysis. Do the plasmid isolation with positive control, by the same E coli strain but different plasmid.
  Following
• Answer added in Biotechnology
  4 Comprehensive list of chemicals that can/shouldn't be autoclaved together/at all?
  By Caleb Radens · Cornell University
  Devrim Demir Dora · University of Illinois, Urbana-Champaign

  Hi Caleb, it is hard to find a full list. You can only find general information about chemicals on web like proteins should be sterilized by only filt... [more]

  Hi Caleb, it is hard to find a full list. You can only find general information about chemicals on web like proteins should be sterilized by only filtration. I recommend you pharmacopeas (USP (American) or EP (European)) that you can find more information about chemicals.
  Following