DR. Joydip Mukherjee

Publications

• Precision Dairy farming-a new outlook in dairying

  De, Pal, Mukherjee

  Livestock Line. 03/2012;
• 1.23

  Impact points

  Effect of supplementation of vitamin E, copper and zinc on the in vitro phagocytic activity and lymphocyte proliferation index of peripartum Sahiwal (Bos indicus) cows.

  A K Dang, S Prasad, K De, S Pal, J Mukherjee, I V R Sandeep, G Mutoni, M M Pathan, M Jamwal, S Kapila, R Kapila, H Kaur, S Dixit, A K Mohanty, B S Prakash

  Journal of animal physiology and animal nutrition. 01/2012;

  To study the effect of vitamin E (VE), copper (Cu) and zinc (Zn) supplementation on the in vitro phagocytic activity (PA) and lymphocyte proliferation response (LPR) of blood neutrophils and lymphocytes, thirty Sahiwal pregnant cows (six in each group) in their late gestation at 30 days before the e... [more] To study the effect of vitamin E (VE), copper (Cu) and zinc (Zn) supplementation on the in vitro phagocytic activity (PA) and lymphocyte proliferation response (LPR) of blood neutrophils and lymphocytes, thirty Sahiwal pregnant cows (six in each group) in their late gestation at 30 days before the expected date of calving were selected from the NDRI experimental herd and supplemented with various micronutrients from 30 days before calving to 45 days after calving. Cows were supplemented individually with VE (1000 IU/cow/day), Cu (20 ppm/cow/day) and Zn (80 ppm/cow/day) and also with a combination of VE, Cu and Zn to study cumulative effect of all micronutrients. One group without any supplementation acted as a control. Blood neutrophils and lymphocytes were isolated and studied for their PA and LPR. Supplementation of micronutrients like VE, Cu, Zn and a combination of all these nutrients significantly (p < 0.01) increased the PA of experimental cows as compared to control (unsupplemented) cows during the pre-partum period. During post-partum, all the micronutrients (VE, Cu, Zn and their combination) showed a significant (p < 0.01) increase in the PA of experimental cows as compared to control cows. Of all the groups, significant (p < 0.01) and maximum PA was observed in the combination group followed by Zn-supplemented group during both the pre- and post-partum period. A significant (p < 0.01) increase in LPR of B lymphocytes was observed in combination-supplemented group during the pre-partum period and during both the pre- and post-partum period in the Cu-supplemented group.

• In Vitro Immune Competence of Buffaloes (Bubalus bubalis) of Different Production Potential: Effect of Heat Stress and Cortisol.

  Joydip Mukherjee, Sujata Pandita, Ruokuobeinuo Huozha, Manju Ashutosh

  Veterinary medicine international. 01/2011; 2011:860252.

  Twelve healthy lactating Murrah buffaloes of similar parity (3rd) between 90 and 120 days of lactation, selected from the herd of National Dairy Research Institute (Karnal, India) and maintained at managemental practices as followed at the Institute they were included in this experiment. The animals... [more] Twelve healthy lactating Murrah buffaloes of similar parity (3rd) between 90 and 120 days of lactation, selected from the herd of National Dairy Research Institute (Karnal, India) and maintained at managemental practices as followed at the Institute they were included in this experiment. The animals were divided into two groups based on their production level in previous lactation. The average milk production level of group 1 and II was 9.3 and 6 lit/day, respectively. Blood was collected from these buffaloes on three occasions 10 days apart. The lymphocytes were separated and cultured in RPMI 1640 medium with PHA-P for 24 h at 37°C in a humidified CO(2) incubator (95% air and 5%  CO(2)). The lymphocyte responsiveness was also evaluated in response to the in vivo heat stress and in vitro cortisol. Mitogen-induced stimulation index was not affected by production level (P < .01). Stimulation index was significantly reduced (P < .01) in both the groups when cortisol was added at 2.0 ng level in the culture. However, in heat-stressed buffaloes stimulation index did not vary despite increasing levels of cortisol, thus indicating that lymphocyte may become cortisol resistant during periods of acute heat stress. The results showed that lymphocyte proliferation response can be effectively used to study buffalo cell-mediated immunity in vitro.

• Effect of supplementation of Vitamin E, Copper and Zinc on the in vitro phagocytic activity and lymphocyte proliferation index of peripartum Sahiwal (Bos indicus) cows

  A.K. Dang, Shiv Prasad, Kalyan De, Shashi Pal, J Mukherjee, Sandeep, I.V.R., G. 12 Mutoni, M.M. Pathan, Manu, Jamwal, S Kapila, R Kapila, H Kaur, Satpal Dixit, A.K. Mohanty, B.S. Prakash

  Journal of animal physiology and animal nutrition. 01/2011;

  To study the effect of Vitamin E (VE), Copper (Cu), Zinc (Zn) supplementation on the in vitro phagocytic activity (PA) and lymphocyte proliferation response (LPR) of blood neutrophils and lymphocytes, thirty Sahiwal pregnant cows (6 in each group) in their late gestation at 30 days before the expect... [more] To study the effect of Vitamin E (VE), Copper (Cu), Zinc (Zn) supplementation on the in vitro phagocytic activity (PA) and lymphocyte proliferation response (LPR) of blood neutrophils and lymphocytes, thirty Sahiwal pregnant cows (6 in each group) in their late gestation at 30 days before the expected date of calving were selected from the NDRI experimental herd and supplemented with various micronutrients from 30 days before calving to 45 days after calving. Cows were supplemented individually with VE (1000 IU/cow/day), Cu (20 ppm/cow/day) and Zn (80 ppm/cow/day) and also with a combination of VE, Cu and Zn to study cumulative effect of all micronutrients. One group without any supplementation acted as control. Blood neutrophils and lymphocytes were isolated and studied for their PA and LPR. Supplementation of micronutrients like Cu, Zn and a combination of all these nutrients significantly (P<0.01) increased the PA of experimental cows as compared to control (unsupplemented) cows during the prepartum period. During postpartum all the micronutrients (VE, Cu, Zn and their combination) showed a significant (P<0.01) increase in the PA of experimental cows as compared to control cows. Of all the groups, significant (P<0.01) and maximum PA was observed in the combination group followed by Zn supplemented group during both the pre and postpartum period. A significant (P<0.01) increase in LPR of B lymphocytes was observed in combination supplemented group during the prepartum period and during both the pre and postpartum period in the Cu supplemented group

• Activity of blood leukocytes around parturition in high producing crossbred cows

  Mandira Chaudhury, Joydip Mukherjee, Suman Kapila, A.K. Mohanty, A.K. Dang

  International Conference On Frontiers in Reproductive Biotechnology, NDRI, Karnal; 01/2011

• Phagocytic activity of blood neutrophils and its relationship with plasma concentration of TNF-α, IL-6 and milk SCC in high and low producing crossbred cows during early lactation

  Sandeep Kaswan, Joydip Mukherjee, Shiv Prasad, A.K. Dang

  Indian Journal of Animal Sciences. 01/2011;

  To study the inter-relationship between plasma concentrations of TNF-α and IL-6 with SCC and phagocytic activity (PA) of blood neutrophils, Blood and milk samples were collected from 16 Karan Fries (KF) cows (8 high producing and 8 low producing) during 100 days of early lactation and from 4 mastiti... [more] To study the inter-relationship between plasma concentrations of TNF-α and IL-6 with SCC and phagocytic activity (PA) of blood neutrophils, Blood and milk samples were collected from 16 Karan Fries (KF) cows (8 high producing and 8 low producing) during 100 days of early lactation and from 4 mastitic KF cows during different days of clinical mastitis. Phagocytic activity (PA) of blood neutrophils was estimated by NBT reductive assay. Concentrations of IL-6 and TNF-α were assayed by ELISA kit. Plasma TNF-α concentration was highest on the day of calving and it declined significantly (P<0.01) in low yielding cows up to 45th day, whereas, it declined non significantly in high yielding cows. Peak values of IL-6 were obtained at calving and during peak yield in low yielding cows, whereas, in high yielding cows, peak values were obtained towards peak yield only. PA was lowest at calving in both the group of cows and then increased significantly (P<0.01) to normalize with the progression of lactation. Plasma concentration of cytokines TNF-α and IL-6 were significantly (P<0.01) higher in mastitic animals as compared to normal animals. Significantly (P<0.01) higher concentration of both the cytokines were observed during the first day of mastitis which later declined by the fifth day of mastitis. PA of blood neutrophils was lowest on day 1 of mastitis and increased significantly (P<0.01) by the fifth day of mastitis. Plasma concentrations of IL-6, TNF-α and milk SCC during different days of mastitis were found to be negatively correlated (P<0.05) with PA of blood neutrophils. This study indicated that the plasma concentration of TNF-α and IL-6 can be used as an indicator of mammary stress around calving in high producing cows.

• Effect of parity and stage of lactation on milk SCC and DLC in native and crossbred cows

  Kalyan De, Joydip Mukherjee, A. K. Dang, Shiv Prasad

  AMERICAN JOURNAL OF FOOD AND NUTRITION (AJFN). 01/2011;

  Milk samples were collected from 157 Crossbred (Karan-Fries) and 38 Sahiwal lactating cows randomly and classified on the basis of parity, stage of lactation. Somatic Cell Counts (SCC) and Differential Leukocyte Count (DLC) of milk samples were measured microscopically. Milk SCC significantly (P<... [more] Milk samples were collected from 157 Crossbred (Karan-Fries) and 38 Sahiwal lactating cows randomly and classified on the basis of parity, stage of lactation. Somatic Cell Counts (SCC) and Differential Leukocyte Count (DLC) of milk samples were measured microscopically. Milk SCC significantly (P<0.05) increased from 3rd to 5th parity in native cows and crossbred cows. No significant variation in milk DLC was found between native and crossbred of different parity, though a significant (P<0.05) increase in neutrophil and significant (P<0.05) decrease in lymphocyte was found in crossbred cows in 3rd and 5th parity in comparison to native cow. . Comparison between milk SCC in native and crossbred cows of different stage of lactation showed non significant changes though it was significantly (P<0.05) higher in crossbred cows than the native cows in mid and late lactation. This study reflects that milk SCC is always higher in crossbred cows. Variation in milk SCC is more when compared with DLC in both the breeds. But for estimating milk quality milk SCC along with DLC gives better picture.

• Alterations in hematology, neutrophilic phagocytic activity and plasma cytokine profile in relation to udder health during different days of clinical mastitis in crossbred Karan Fries cows

  Sandeep Kaswan ● Joydip Mukherjee ● Shiv Prasad ●A.K. Mohanty ● A.K. Dang

  Indian Journal of dairy science. 01/2011; 64(3):247-251.

  Ten Karan Fries (Holstein Friesian x Tharparkar) cows affected with clinical mastitis were selected from the heath complex of institute’s cattle yard. Blood and milk samples were collected from all these cows during different days of clinical mastitis (from the day of onset to 5th day of infection).... [more] Ten Karan Fries (Holstein Friesian x Tharparkar) cows affected with clinical mastitis were selected from the heath complex of institute’s cattle yard. Blood and milk samples were collected from all these cows during different days of clinical mastitis (from the day of onset to 5th day of infection). Hematological parameters were analysed by standard hematological procedure. Phagocytic Index (PI) of neutrophils were measured by NBT reductive assay. Cytokine (TNF-α and IL-6) levels in plasma were estimated by ELISA kit. The udder health was monitored by Somatic Cell Counts (SCC) and milk Differential Leukocyte Counts (DLC). Milk compositional change was evaluated by milk analyser. The results indicated that in crossbred KF cows, mastitis had no bearing on hematology, PI of blood neutrophils was lowest on the first day which later increased significantly (P<0.01) up to fifth day of clinical onset of mastitis. SCC values were significantly (P<0.01) highest at first day of mastitis. Milk neutrophil percentage was highest at first day of mastitis and declined significantly (P<0.01) up to fifth day of mastitis, whereas macrophage count was lowest on first day of mastitis which later increased significantly (P<0.01) up to fourth day of mastitis. Lymphocyte count was significantly (P<0.01) low on first day of mastitis. Fat, lactose and SNF contents of mastitic milk were significantly (P<0.01) low on day first which later increased towards normalcy. TNF-α and IL-6 concentrations were significantly (P<0.01) high on first day of mastitis which later declined continuously up to fifth day of mastitis. There was a positive correlation between TNF-α, IL-6 and milk SCC, whereas, these are negatively correlated with PI of blood neutrophils in mastitis KF cows

• Effect of nitric oxide compounds on in vitro immune competence of lactating Murrah buffaloes

  J. Mukherjee, S. Pandita, R. Huozha

  Journal ofAgricultural Science and Technology. 01/2011;

  To study the effect of nitric oxide on lymphocyte proliferation response in lactating buffaloes, twelve healthy lactating murrah buffaloes of similar parity (3rd) between 90-120 days of lactation, selected from herd of National Dairy Research Institute and maintained at managemental practices as fol... [more] To study the effect of nitric oxide on lymphocyte proliferation response in lactating buffaloes, twelve healthy lactating murrah buffaloes of similar parity (3rd) between 90-120 days of lactation, selected from herd of National Dairy Research Institute and maintained at managemental practices as followed at the institute, were included in this experiment. The average milk production level of group I and II was 9.33 and 6.0 lit/day respectively. Blood was collected from these buffaloes on three occasions 10 days apart. The lymphocytes were separated by density gradient centrifugation in lymphocyte separation medium and cultured in RPMI 1640 medium with PHA-P (phytohaemagglutinin-P) for 24h at 37oC in a humidified CO2 incubator (95% air and 5% CO2). The lymphocyte responsiveness in vitro was also evaluated in response to different nitric oxide compounds viz SNAP (S-Nitroso-N-acetylpenicillamine), LNAME (N-nitro-L-arginine methyl ester) and NMMA (L-Mono-methyl-arginine) by MTT assay. Lymphocytes were found to be responsive to nitric oxide compounds. While the blastogenic response was significantly reduced at 10 mM (P<0.01) by SNAP (nitric oxide donor), the NOS (Nitric oxide Synthase) inhibitors (both L-NAME, NMMA) were effective at as low as 0.2mM concentration (P<0.01). The study demonstrates the presence of an active NOS system in buffalo lymphocytes.

• Immune activity of milk leukocytes during early lactation in high and low yielding crossbred cows

  J Mukherjee, A. K. Dang

  Milchwissenschaft. 01/2011; 66:384-388.

• Effect of Micronutrient Supplementation on the in vitro Phagocytic Activity of Blood and Milk Neutrophils of Cows

  Joydip Mukherjee, A.K. Dang

  The 7th Asia Pacific Conference on Clinical Nutrition, Thailand; 01/2011

• Comparative blood profile of high and low yielding Karan fries cows during early lactation

  Sandeep Kaswan, Jyodip Mukherjee, Shiv Prasad, A.K. Dang

  Indian Journal ofAnimal Research. 01/2011;

  Hematological parameters associated with immunity and general health were estimated in high (EPA >5000kg/305d, n=8) and low yielding (EPA <3500kg/305d, n=8) Karan Fries (KF) (Holstein Friesian x Tharparkar) cows during their early lactation. Blood samples were collected once in a week during e... [more] Hematological parameters associated with immunity and general health were estimated in high (EPA >5000kg/305d, n=8) and low yielding (EPA <3500kg/305d, n=8) Karan Fries (KF) (Holstein Friesian x Tharparkar) cows during their early lactation. Blood samples were collected once in a week during early lactation period i.e. from 1st to 14th week postpartum. Hematological parameters included under study are Hemoglobin, Packed cell volume (PCV), Total erythrocyte count (TEC), Total leukocyte count (TLC), Differential leukocyte count (DLC), blood indices and phagocytic activity (PA) of blood neutrophils. Hemoglobin concentration, Eosinophil count, TEC and PA of blood neutrophils were significantly (P<0.01) higher in low yielding cows when compared to high yielding cows. These values also differed significantly (P<0.01) during different weeks postpartum. Whereas, Mean corpuscular volume (MCV) values were significantly (P<0.01) higher for high yielding cows as compared with low yielding cows. No significant difference were found for other parameters under study i.e. PCV, TLC, blood DLC (except eosinophils), Mean corpuscular hemoglobin (MCH) and Mean corpuscular hemoglobin concentration (MCHC). Comparatively lower PA in high yielding cows could be one of the possible explanation for increased occurrence of udder infections i.e. mastitis in high yielder cows.

• Feed back inhibition of lactation: An autocrine regulator of milk secretion

  Joydip Mukherjee, Mandira Chaudhury, Kalyan De

  Vetscan- Online Journal. 01/2011; 6:86.

  Frequency of milking or complete evacuation of udder regulates the rate of milk secretion by a mechanism which is local, chemical and inhibitory in nature. The active whey protein, which we term as FIL (Feedback Inhibitor of Lactation), decreases milk secretion temporarily when introduced into a mam... [more] Frequency of milking or complete evacuation of udder regulates the rate of milk secretion by a mechanism which is local, chemical and inhibitory in nature. The active whey protein, which we term as FIL (Feedback Inhibitor of Lactation), decreases milk secretion temporarily when introduced into a mammary gland of lactating animals. FIL is synthesized by mammary epithelial cells, and secreted vectorially together with other milk proteins. N-terminal amino acid sequencing indicated that it is a hitherto unknown protein. Local control of mammary secretion during weaning, and after peak lactation in dairy animals, may be due to the actions of this inhibitor, and can be manipulated by frequency of milk removal.

• A comparative study between the phagocytic activity of milk neutrophils isolated from fore and rear quarters of crossbred cows

  Joydip Mukherjee, Kalyan De, A.k. Dang

  Indian veterinary journal. 01/2011; 88((10)):29-31.

• Effect of different physiological stages and managemental practices on milk somatic cell counts of Murrah buffaloes

  Kalyan De, Joydip Mukherjee, Shiv Prasad, A.K. Dang

  Buffalo Bulletin. 01/2011; volume 30.

• More light equals more milk!...Effect of photoperiods on Milk production in Dairy Cattle

  J. Mukherjee, M. Chaudhary, De, Kalyan, A.K. Dang, J.P Sehgal

  Agrovet Buzz. 01/2011; 4:47-49.

• Comparative study of milk composition and cellular parameters in high and low yielding crossbred cows during early lactation period

  Sandeep Kaswan, Joydip Mukherjee, Shiv Prasad, A.K. Dang

  Indian veterinary journal. 01/2011; 08(11):92-93.

  Milk parameters associated with immunity and quality of milk were estimated in high and low yielder Karan Fries cows during early lactation. Milk samples were collected from all the experimental animals once every week. Milk lymphocyte count and milk fat percentage were significantly higher in low y... [more] Milk parameters associated with immunity and quality of milk were estimated in high and low yielder Karan Fries cows during early lactation. Milk samples were collected from all the experimental animals once every week. Milk lymphocyte count and milk fat percentage were significantly higher in low yielding cows when compared to high yielding cows. These values also differed significantly during different weeks postpartum. Whereas, SCC and milk macrophage counts were significantly higher for high yielding cows as compared with low yielding cows, no significant difference were

• Oxidative Stress on Spermatozoa and its Prevention during Cryopreservation

  Gulab Chandra, Joydip Mukherjee, Anjali Aggarwal, Muneedra Kumar

  98th Indian Science Congress, Chenni; 01/2011

  “Oxidative stress” (OS) is a condition associated with an increased rate of cellular damage induced by oxygen and oxygen-derived free radical (an oxygen moleculecontaining one or more unpaired electrons in atomic or molecular orbitals), not balance by antioxidants. Free radicals and reactive oxygen ... [more] “Oxidative stress” (OS) is a condition associated with an increased rate of cellular damage induced by oxygen and oxygen-derived free radical (an oxygen moleculecontaining one or more unpaired electrons in atomic or molecular orbitals), not balance by antioxidants. Free radicals and reactive oxygen species (ROS) seek to participate in chemical reactions that relieve of their unpaired electron, resulting in the oxidation of lipids in membranes, amino acids in proteins and carbohydrates within nucleic acids which in turn down regulates the steroidogenic activity leading to altered testicular function, cause infertility in males. Spermatozoa are under a continuous influence of OS because of excessive generation of ROS, cause infertility in males. Spermatozoa posses an inherent but limited capacity to generate ROS which may help the fertilization process. Antioxidants improve the motility and fertilizing ability of spermatozoa. A balance between the benefits and risks from ROS and antioxidants appears to be necessary for the survival and normal functioning of spermatozoa. Antioxidants in extender may improve the quality of frozen-thawed spermatozoa.

• Immunocompetence of mammary gland during different stages of lactation cycle in high yielding Karan Fries (KF) crossbred cows

  Joydip Mukherjee, Nishant Varsney, A.K. Mohanty, A.K.Dang

  XX Annual Conference of Society of Animal Physiologist of India & International Symposium, Kolkata, Kolkara; 01/2011

• Immune activity of colostrum in high yielding Karan Fries cows

  Mandira Chaudhury, Joydip Mukherjee, A.K. Dang

  Indian veterinary journal. 01/2011;