Publications

  • LUCRĂRI ŞTIINŢIFICE MEDICINĂ VETERINARĂ TIMIŞOARA. 01/2012; XLV.
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    CRINA MOȘNEANG, C. IGNA
    LUCRĂRI ŞTIINŢIFICE MEDICINĂ VETERINARĂ TIMIŞOARA. 01/2012; XLV.
  • LUCRARI STIINTIFICE MEDICINA VETERINARA IASI. 01/2011; 54(4):9-15.
  • LUCRARI STIINTIFICE MEDICINA VETERINARA IASI. 01/2011; 54(3):372-374.
  • LUCRĂRI ŞTIINŢIFICE MEDICINĂ VETERINARĂ , TIMIŞOARA. 01/2011; XLIV.
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    ABSTRACT: In this study we have evaluated OCD healing in dogs, using kinematic analysis performed at 90 days after surgery (collagenic scaffolds loaded with autologus chondroblasts). The results of kinematic analysis were compared with those obtained in healthy animals and with patterns obtained in day 14 after induction stifle OCD by surgery (3). Incomplete cover or uncover of screws head by de novo cartilage resulted in persistence of an irritative factor associated with maintaining of discrete changes in kinematic pattern of the hind limbs joints. In control group individuals, joint movements changed during the time, being characterized by maintaining or even emphasizing of the specific kinematic pattern. That suggests the persistence of joint cartilage damages. Having capacity to detect subtle changes in joints motion, with dynamic monitoring of these, kinematic analysis allows assessment of joint movement re-establishment after various surgical or medicamentary treatments. Dog's kinematic analysis was used especially for evaluation of gait anomalies associated with hip dysplasia (7) and rupture of anterior cruciate ligament (4, 5, 8), both diseases inducing gait altering by changes in coxofemoral, femorotibial and tarsal joints. Other important application of kinematic analysis is assessment of different surgical techniques used for cruciate ligament rupture treatment (1, 6). Bollinger et al. (2) were used kinematic analysis combined with force plates in purpose to assess response of dogs diagnosed with hip dysplasia at symptomatic osteoarthritis treatment by acupuncture.
    01/2009;
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    ABSTRACT: The purpose of this study was the comparison between capacity of bone marrow cells on the one hand, and of mature lymphocytes and monocytes on the other hand, to induce immunotolerance by inoculation in COBB 500 hybrids at different embryonic stages. Antigenic material was obtained by aspiration of tibial and femoral bone marrow, respectively by blood sampling from the donor birds (Ross hybrids).We used 240 embryonated eggs: 60 eggs for in ovo inoculation with bone marrow cells suspension in the 5 th day of embryo development and 60 for inoculation in the same way and day with mature lymphocytes and monocytes suspension; the rest of biologic material was inoculated with these suspensions in allantoidal vessel in the 8 th day of the embryo. Seven days after the full-thickness skin grafts transplantation (from the donor birds) the lymphocyte T subsets of the recipient birds were determined by flow cytometry and immunomagnetic sorting. Also, all the skin grafts were periodically monitored for macroscopic characteristics. This experiment demonstrates the superiority of bone marrow cells in immunotolerance induction in birds after inoculation in 5 th day of embryonic development.
    Veterinary Medicine. 01/2008; 65:1843-5270.
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    ABSTRACT: The purpose of the present study was to evaluate the safety of use the propofol-thiopental mixture in brachycephalic dogs and the recovery quality when it is used for perform an ancillary diagnostic test of spinal condition, myelography. Nine brachycephalic dogs were anesthetized by bolus IV administration of 0,5 ml/kg propofol 1%-thiopental 2,5% 1:1 mixture, followed by repeated supplementations of 20% of the initial dose every 10 seconds until endotracheal intubation was possible and an adequate plane of anesthesia that permitted performing myelography procedure in safe condition was achieved. Pulse quality, capillary refill time, mucous membrane color, respiratory rate, oxygenation, the length of anesthetic effect, the quality of recovery and total volume of anesthetic administered were recorded.
    Veterinary Medicine. 01/2008; 65:1843-5270.
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    ABSTRACT: For a better management of critically ill patients we use a modified trauma score applied from 2007 with ranges between 6 to 27 points. It allows dividing a case in critical or non-critical and improving the reaction time and the use of emergency resources. It has inherent limitations in pediatric aged dog group and must be improved in the future. Trauma is a major health problem and a leading cause of death in small animals. Many deaths can be prevented through an organized approach to care, which includes a careful evaluation, prompt resuscitation and definitive treatment (4, 5, and 7). For triage, especially for identification of most severely injured patients, and for prognosis it is used a trauma score. In human medicine at the current time the issue of which is the best trauma scoring model remains unresolved (1, 2, 3, and 6). The application of trauma evaluation score, modified and adapted for small animals, has introduced in our department since 2007. The goal of this retrospective study is to asses its applicability and to identify its limitations.
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    ABSTRACT: The purpose of this study was to establish the influence of hypertonic saline solution 7,5% perfusion on erythrocyte function and morphology in condition of post hemorrhagic hypovolemic shock state on dogs. The research was carried out on 22 indefinite half-breed dogs on which under anesthesia, were induced the hemorrhagic shock, and the reequilibration therapy with hypertonic saline solution administrated by intravenous or intra-arterial route was applied. In order to highlight the changes of the erythrocyte diameter were prepared some blood smears, colored May-Grünwald-Giemsa, that were examined with a 90 object lens. The pulse oximetry assessing brings us information about the gas exchange at pulmonary level and indirectly about erythrocyte capacity to binding and carrying oxygen. The obtained results demonstrates the fact that the use of hypertonic saline solution does not compromise the erythrocyte function and in context of microcirculatory changes associated with shock state the decrease of erythrocyte diameter is favorable.
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    ABSTRACT: Introduction.Recurrent haemarthroses will inevitably lead to significant hypertrophic synovitis in patients with haemophilia (PwH), progressive joint cartilage degradation, ultimately resulting in haemophilicarthropathy with significant functional impairment of the affected joints. The degree of haemophilicsynovitis is directly related to anincrease in bleeding frequency in the affected joint.Synoviorthesis or non-surgical synovectomy is aa therapeutic method which consists in injection of a substance in to the joint, which acts on the synovial membrane by means of a fibrosis that constricts the subsynovial plexus and thus prevents future bleeding. There are two groups of preparations: chemical and radioactive isotopes. Material and methods.Twenty two albino New Zeeland White specific pathogen free rabbits were used for the study. From one of them synovial membrane was harvested from both knees and send for anatomopathological examination. The remaining rabbits were injected into both knees once a week for 4 weeks with autologous blood (2 ml) harvested from the safenous vein, mimicking the pattern of repeated hemarthroses that the patients with haemophilia experience. After this synovial membrane and articular cartilage were harvested from both knees from a second rabbit and send for anatomopathological examination to observe intraarticular damage. The remaining rabbits were divided in two groups and oxytetracycline (200 mg/ml) and aetoxysklerol 1% (20 mg/ml) were injected into their left knee, while the right knee was injected with saline solution in each group of rabbits once a week for a period of 4 weeks. After that all rabbits were euthanized and synovial membrane and joint cartilage were harvested. Theanatomopathological specimens were stained with hematoxylin-eosin and examined under optic microscopy. Results and discussions. No problems related to the procedures were encountered, except for discrete ambulatory problems after injection of blood into the knee joints. The general status of all rabbits was good during the whole period of the experiment. Repeated hemarthroses into the knee resulted in proliferation of the synoviocits with inflammatory signs resembling acute synovitis and higher magnification levels in optical microscopy revealed presence of hemosiderin and inflammatory cells. This proliferation of the synovium and neovascularization of the subsynovial layer results in an inflamed, villous, friable and highly vascular synovial tissue.The specimens from the rabbits injected with oxytetracycline and aetoxysklerol showed fibrosis, regeneration of the synovial tissue and controlled reparation, with slide enlargement of synovial tissue, less irrigated, and less prone to rebleed in 100% of cases. Conclusions. Synoviorthesis should be the first choice of treatment for persistent synovitis of the joints in patients with haemophilia. It is a simple procedure, which eliminates the risks associated with surgery and is also cost-efficient. Preliminary experimental data show a good efficiency of both oxytetracycline and aetoxysklerol as materials used for chemical synovectomy in rabbits with acute synovitis of the knees. There is still need for further experimental data gathering and dosage adjusting before optimal use of these substances in the treatment of haemophiliacs. Introduction Recurrent haemarthroses will inevitably lead to significant hypertrophic synovitis in patients with haemophilia (PwH) , progressive joint cartilage degradation, ultimately resulting in haemophilicarthropathy with significant functional impairment of the affected joints. The degree of haemophilicsynovitis is directly related to anincrease in bleeding frequency in the affected joint(1). There are two basic types of procedures for synovial control: medical synovectomy (or synoviorthesis) and surgical synovectomy (open or arthroscopic.) It is commonly accepted today that synoviorthesis is the procedure of choice, and that surgical synovectomy should be performed only if a number of consecutive synoviortheses fail to stop or diminish the frequency of recurrent haemarthrosis(2,3).
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    ABSTRACT: Bone defects repair with bio-composite materials represents a viable alternative for autologous osseous grafts, being a major and current concern for numerous researcher teams (1, 2, 3, 4, 6, 7, 8, 9, 10). In the present study we proposed to verify the possibility to use collagen scaffolds with hydroxyapatite support loaded with osteoblasts, arises by cultivation from mesenchymal stem cells, for tibia bone defects repair on dogs.
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    ABSTRACT: The research was carried out on 104 dogs submitted in surgery clinic for programmed surgical interventions. These patients were prepared for intubation using in premedication acepromazine or xylazine and for induction of general anesthesia thiopental or propofol. For observe cardiac dysrhythmias the ECG electrodes were places in Lead II. During intubation in 11.53% of dogs were recorded isolated premature ventricular contraction (75% of cases) and 5-10 seconds periods of transient ventricular tachycardia (25% of cases). 18.75% of dogs that received xylazine-propofol combination show up alteration of T wave associated with ST segment infra-disleveled. There is a low incidence of cardiac arrhythmias during orotracheal intubation meaneuver. These changes were transient and have no clinical significance. The perioperative evaluation and monitoring of cardiac activity is very important for all patients. It is known that anesthetic drugs may cause alterations in cardiac function by decreasing cardiac output and mean blood pressure, reducing venous return, inducing different types of arrhythmias and sensitivity of myocardium for circulating cathecolamines (5). For a safe anesthesia cardiac control through electrocardioscopy is necessary. There are some data regarding hemodinamic response to the laringoscopy and endotracheal intubation (4, 6, 8, 10) but there is a lake of information and electrocardiographic records regarding to orotracheal intubation and correlation with cardiac arrhythmias in dogs (9). However it is difficult to choose of what changes to look for and when these changes may be significant (3) Further, the animal position on the operation table not corresponds with the standard ECG recording position, and in this case it is important to note what the tracing is like at the beginning of the anesthetic procedure and watch for changes.
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    ABSTRACT: Sick or traumatized cartilage has a limited regeneration capacity. Tisular engineering catalogues among possible therapeutically methods (1, 5) for the articular condral defects repair using scaffolds implants loaded with condro-progenitor cells (3, 8, 10, 12). Glucosamine is part of normal cartilage. Studies show that glucosamine can reduce pain in osteoarthritis and improve articular performances (9,11). In the present study we will follow the influence of glucosamine on the inflammatory process and on articular cartilage regeneration in dogs with articular chondral defects surgically induced/treated by synovial fluid analysis.
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    ABSTRACT: In this study three-dimensional computer-asisted kinematic analysis was utilized to describe gait geometry in healthy common breed dogs for building a database which can be used as a model of musculoskeletal complex function. To determine the movement o scapulohumeral, elbow, carpal, coxofemural, femorotibial and tarsal joints in 3D space, opto-electronic system based on emission and reception of ultrasounds was used.
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    ABSTRACT: The research was carried on 32 bitches brought for ovariohysterectomy. For analgesia butorphanol was used in three different doses (0.2 mg/kg -group 1, 0.3 mg/kg -group 2 and 0.4 mg/kg -group 3. During the intraoperative period an acute heart rate, respiratory rate or mean arterial pressure increase equal or higher than 20 per cent of the previous value was considered a sign of intraoperative pain. Elective ovariohysterectomy is the most frequently common surgical procedure performed in companion animal practice (3) and the Centre for Veterinary Medicine of the US Food and Drug Administration considers ovariohysterectomy to cause moderate pain, making it suitable for clinical studies of analgesia (1). The relief of pain is one of the primary objectives of anaesthesia and the ideal analgesic should be easy to administer, long lasting, and effective. Similarly to the other agents of its class, butorphanol has analgesic properties acting on kappa opioid receptors. It is considered a potent analgesic agent with a favourable side effect profile (7). The analgesic effect is usually considered to be dose-dependent. They are few data available in literature regarding the dose-effect relationship of butorphanol's analgesic effects in bitches' ovariohysterectomy. This study represents an attempt to compare the efficiency in controlling visceral pain with butorphanol at doses ranging from 0.2 to 0.4 mg/kg.
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    ABSTRACT: The research was carried on 17 adult dogs underwent osteosynthesis. In the anesthetic premedication were included butorphanol + acepromazine + ketamine (group B) or tramadol + acepromazine + ketamine (group T). Induction and maintenance of anesthesia was the same for both groups. Post-operative analgesia was assessed using a dynamic interactive visual analogue scale. Rescue analgesia was provided with butorphanol or tramadol. Orthopedic procedures cause severe and prolonged pain and to provide an adequate level of comfort more rescue analgesia were necessary to dogs in group B compared with dogs in group T. Providing analgesia in orthopaedic intervention is a constant and important concern, having a direct reflection on postoperative evolution. Arrangements for obtaining it are numerous and are based on many types of pharmacological agents. Tramadol is a centrally acting analgesic, and is increasingly used for analgesia in dogs. It has only recently gained significant attention as an analgesic in dogs despite its having been used in humans in Germany since 1977 and in North America since the mid 1980's (2). Butorphanol is frequently used to provide analgesia in small animal practice, but the results regarding the efficacy of pain control are different (3). The purpose of this study was to determine the effect of premedication with tramadol or butorphanol on post-operative pain following osteosynthesis in dogs.
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    ABSTRACT: This study was made to evaluate "in vitro" the osteogenic potential and the mineralization capacity of subperiosteal stem cells on dog, comparing two isolation cells methods: from explant and by digestion. Periosteal fragments were detached from middle femoral diaphysis of eight common breed dogs. Half of the probes were used as explants and the other half were submitted to artificial digestion. The periosteal cells were than isolated, cultured and differentiated in DMEM environment. The osteogenic capacity evaluation of cellular cultures was made by alkaline phosphatase activity determination and the mineralization capacity of extracellular matrix was evaluated by Von Kossa reaction. We have concluded that both periosteal cells isolation methods taken into study can be used for osteoprogenitor cells obtaining. In the presence of osteoinductive factors from the differentiation environment, the periosteal cells in the cultures start to differentiate in osteoblasts. Subperiosteal cellular layer contains stem cells capable to form lamellar bone tissue (4), helping in both diameter growth of cortical bones and in bone defects (fractures) healing. Although on some laboratory animal species and humans, cultivation techniques are known and presented (1, 6, 9), these were less investigated on dogs (10). In this study we have proposed to evaluate "in vitro" the osteogenic potential and the mineralization capacity of subperiosteal stem cells on dog, comparing two isolation methods: from explant and by digestion.
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    ABSTRACT: Performing preliminary studies regarding cell biology, osteoarticular biomechanics and scaffolds parameters. Isolation and cultivation of cell types required for seeding on the 3D supports. Elaboration of technologies for achieving scaffolds in osteoarticular reconstruction. Seeding and cell culture on 3D supports. In vivo animal experimental studies concerning the applicability of the developed techniques. Achievement of protocols required for moving on to clinic assessment of used biomaterials and procedures. Sick or traumatized cartilage has a limited regeneration capacity. Tisular engineering catalogues among possible therapeutically methods (1, 4, 7, 11) for the articular chondral defects repair using scaffolds implants loaded with chondro-progenitor cells (3, 5, 9, 10, 12, 13). In the present study we proposed in vivo checking of the possibility to use collagenic scaffolds (with or without hydroxyapatite support) loaded with chondroblasts, arise by cultivation from mesenchymal stem cells, for articular chondral defects repair on dogs.
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    ABSTRACT: Mesenchymal stem cells (MSC) posses the ability to differentiate into any type of somatic cells if properly stimulated. This study documented the effects of using mesenchymal stem cells loaded scaffolds in repairing large bone and cartiladge defects in dogs. The innovative aproach to osteoarticular reconstruction in dogs by using auto-or alogenic MSC loaded scaffolds, opens up new possibilities for treating diverse conditions with bioreactive materials and cells. The MSC were obtained from the humeral bone marrow aspirates. The multipotent cells underwent a specific cultivation and differentiation protocol, derived from the standard procedure. The protocol was altered to best fit the cells behaviour as it follows: the cultivation period needed to reach the 60-80% junction point, differed from dog to dog as well as the potency of the cell cultures, this determining the moment of their passage; the MSC were poured on the scaffolds and only then the diffentiation medium was added, this allowing the cells to differentiate on the osteogenic and osteoinductive material, thus obtaining a better cell load and disposition of the bioactive scaffold.

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