Claudia Anthony

Questions and Answers (55) View all

• Answer added in PCR
  4 PCR to identify Wild type and Mutant Isolates
  By Claudia Anthony · University of Malaya
  Claudia Anthony · University of Malaya

  Hi Guru, Thank you for your reply.So how do I conduct this PCR? 

  Hi Guru, Thank you for your reply.So how do I conduct this PCR?
  Following
• Question asked in PCR
  4 PCR to identify Wild type and Mutant Isolates
  What is a mismatch primer and what is its function? I need to identify SNPs and for that design specific primers. But instead of the usual forward and... [more]
  What is a mismatch primer and what is its function? I need to identify SNPs and for that design specific primers. But instead of the usual forward and reverse, it appears I also need to design an internal primer. And when I run my gel, I may then get one band for the wildtype, and 2 for the mutant or vice versa. Why is this?
  By Claudia Anthony · University of Malaya
  Following
• Question asked in Karyotyping
  5 SNPs Identification
  I'd like to know how to compare the sequence results of a particular gene of various isolates against the parent strain and identifying SNPs? Meaning,... [more]
  I'd like to know how to compare the sequence results of a particular gene of various isolates against the parent strain and identifying SNPs? Meaning, I'd like to know how I go about comparing a particular gene of one isolate against the parent strain, in this case P vivax Sal-1 and finding differences in amino acids at a particular codon, if any.
  By Claudia Anthony · University of Malaya
  Following
• Answer added in Methods
  34 Amplifying LARGE gene fragments
  By Claudia Anthony · University of Malaya
  Claudia Anthony · University of Malaya

  David, What is the left primer and right primer. Is there a particular one representing the forward and reverse or are they to be chosen by me. Also,... [more]

  David, What is the left primer and right primer. Is there a particular one representing the forward and reverse or are they to be chosen by me. Also, how do I use Primer3?
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• Answer added in Methods
  34 Amplifying LARGE gene fragments
  By Claudia Anthony · University of Malaya
  Claudia Anthony · University of Malaya

  I was able to upload with Mozilla. Unfortunately, I'm only able to upload one pic at a time and so you would find 4 different posts. If there's more... [more]

  I was able to upload with Mozilla. Unfortunately, I'm only able to upload one pic at a time and so you would find 4 different posts. If there's more info that's required, I'd be more than happy to share. Help is much appreciated! P/S:Excuse the typos that you will find in the 4 posts Claudia
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