Chariya Hahnvajanawong

Publications (20) View all

• Article: Involvement of p53 and nuclear factor-[kappa]B signaling pathway for the induction of G1-phase cell cycle arrest of cholangiocarcinoma cell lines by isomorellin.

  Chariya Hahnvajanawong, Supaluk Ketnimit, Kovit Pattanapanyasat, Natthinee Anantachoke, Banchob Sripa, Khosit Pinmai, Wunchana Seubwai, Vichai Reutrakul
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  ABSTRACT: Cell cycle arrest is closely linked to apoptosis. Isomorellin-a caged xanthone isolated from Garcinia hanburyi-induced apoptosis in cholangiocarcinoma (CCA) cell lines. To elucidate potential anticancer mechanisms, we investigated the effects of isomorellin on the growth, cell cycle progression, cell cycle regulated protein expression and nuclear factor-[kappa B] (NF-κB) activation of KKU-100 and KKU-M156 CCA cell lines; using sulforhodamine B assay, flow cytometry and Western blot analysis. The growth of both CCA cell lines was significantly inhibited by isomorellin treatment in a time- and dose-dependent manner. The respective IC(50) value of isomorellin for KKU-100 cells was 6.2±0.13, 5.1±0.11 and 3.5±0.25 μM at 24, 48 and 72 h. By comparison, the respective IC(50) value for KKU-M156 cells was 1.9±0.22, 1.7±0.14 and 1.5±0.14 μM at 24, 48 and 72 h. The growth inhibition of CCA cells by isomorellin was through the G0/G1 phase arrest mediated by inhibition of NF-κB activation, up-regulation of p53, p21 and p27 and down-regulation of cyclin D1, cyclin E, Cdk4 and Cdk2 protein levels. Our research suggests that isomorellin induces cell cycle arrest and apoptosis in CCA cell lines through p53 and the NF-κB-signaling pathway. The growth inhibitory potential of isomorellin was comparable to that of gambogic acid. Isomorellin shows potential as a therapeutic agent against human cholangiocarcinoma.
  Biological & Pharmaceutical Bulletin 08/2012; · 1.66 Impact Factor
• Article: Effects of Helicobacter pylori γ-Glutamyltranspeptidase on Apoptosis and Inflammation in Human Biliary Cells.

  Wongwarut Boonyanugomol, Chariya Chomvarin, Jea-Young Song, Kyung-Mi Kim, Jung-Min Kim, Myung-Je Cho, Woo-Kon Lee, Hyung-Lyun Kang, Kwang-Ho Rhee, Banchob Sripa, Chariya Hahnvajanawong, Seung-Chul Baik
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  ABSTRACT: Several studies have reported the presence of H. pylori in individuals with hepatobiliary diseases, but in vitro and in vivo studies are still needed. Here, we determined the effects of H. pylori γ-glutamyltranspeptidase (GGT) on the induction of apoptosis and IL-8 production in a human cholangiocarcinoma cell line (KKU-100 cells). Cell viability and DNA synthesis were examined by MTT and BrdU assays, respectively. RT-PCR and western blot analysis were performed to assess gene and protein expression, respectively. IL-8 secretion in KKU-100 cells was measured by ELISA. Exposure to the H. pylori ggt (+) strain decreased KKU-100 cell survival and DNA synthesis when compared with cells exposed to the H. pylori ggt mutant strain. Treatment with recombinant H. pylori GGT (rHP-GGT) dramatically decreased cell survival and DNA synthesis, and stimulated apoptosis; these features corresponded to an increased level of iNOS gene expression in KKU-100 cells treated with rHP-GGT. RT-PCR and western blot analyses revealed that rHP-GGT treatment enhanced the expression of pro-apoptotic molecules (Bax, Caspase-9, and Caspase-3) and down-regulated the expression of anti-apoptotic molecules (Bcl-2 and Bcl-xL). The extrinsic-mediated apoptosis molecules, including Fas and activated Caspase-8, were not expressed after treatment with rHP-GGT. Furthermore, rHP-GGT significantly stimulated IL-8 secretion in KKU-100 cells. Our data indicate that H. pylori GGT might be involved in the development of cancer in hepatobiliary cells by altering cell kinetics and promoting inflammation.
  Digestive Diseases and Sciences 05/2012; 57(10):2615-24. · 2.12 Impact Factor
• Article: Helicobacter pylori in Thai patients with cholangiocarcinoma and its association with biliary inflammation and proliferation.

  Wongwarut Boonyanugomol, Chariya Chomvarin, Banchob Sripa, Vajarabhongsa Bhudhisawasdi, Narong Khuntikeo, Chariya Hahnvajanawong, Amporn Chamsuwan
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  ABSTRACT: To investigate whether Helicobacter spp. infection and the cagA of H. pylori are associated with hepatobiliary pathology, specifically biliary inflammation, cell proliferation and cholangiocarcinoma (CCA). Helicobacter species including H. pylori, H. bilis and H. hepaticus were detected in the specimens using the polymerase chain reaction (PCR). Biliary inflammation of the liver and gallbladders was semi-quantitatively graded on hematoxylin and eosin (H&E)-stained slides. Biliary proliferation was evaluated by immunohistochemistry using the Ki-67-labelling index. Helicobacter pylori was found in 66.7%, 41.5% and 25.0% of the patients in the CCA, cholelithiasis and control groups (P < 0.05), respectively. By comparison, H. bilis was found in 14.9% and 9.4% of the patients with CCA and cholelithiasis, respectively (P > 0.05), and was absent in the control group. The cagA gene of H. pylori was detected in 36.2% and 9.1% of the patients with CCA and cholelithiasis, respectively (P < 0.05). Among patients with CCA, cell inflammation and proliferation in the liver and gallbladder were significantly higher among those DNA H. pylori positive than negative. The present findings suggest that H. pylori, especially the cagA-positive strains, may be involved in the pathogenesis of hepatobiliary diseases, especially CCA through enhanced biliary cell inflammation and proliferation.
  HPB 03/2012; 14(3):177-84. · 1.60 Impact Factor
• Source

  Available from: Somdej Kanokmedhakul

  Article: Cytotoxic lasiodiplodin derivatives from the fungus Syncephalastrum racemosum.

  Mongkol Buayairaksa, Somdej Kanokmedhakul, Kwanjai Kanokmedhakul, Panawan Moosophon, Chariya Hahnvajanawong, Kasem Soytong
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  ABSTRACT: Chemical investigation of fungal biomass of the fungus Syncephalastrum racemosum led to the isolation of new natural products (3R),(5S)-5-hydroxy-de-O-methyllasiodiplodin (1), 6-oxode-O-methyllasiodiplodin (2), in addition to five known compounds, de-O-methyllasiodiplodin (3), lasiodiplodin (4), (3R),(5R)-5-hydroxy-de-O-methyllasiodiplodin (5), ergosterol (6), and ergosterol peroxide (7). Their structures were elucidated by spectroscopic techniques. The absolute configuration of 1 was determined by a modified Mosher's method. Compound 1 showed cytotoxicity against cholangiocarcinoma, KKU-M139, KKU-M156, and KKU-M213 cell lines with IC(50) values in the range of 14-19 μg/mL, while 3 showed cytotoxicity against KB, BC1, and NCI-H187 cell lines with IC(50) values of 12.67, 9.65, and 11.07 μg/mL, respectively.
  Archives of Pharmacal Research 12/2011; 34(12):2037-41. · 1.59 Impact Factor
• Article: Cytotoxicity of chemical constituents from the stems of Dalbergia parviflora.

  Uraiwan Songsiang, Chariya Hahnvajanawong, Chavi Yenjai
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  ABSTRACT: Three new compounds, dalberpene, dalparvinene B and dalparvone B, as well as 12 known compounds were isolated from the heartwood of Dalbergia parviflora. Isoflavanone 13 showed strong cytotoxicity against KB, MCF-7 and NCI-H187 cell lines with IC₅₀ values ranging from 3.5 to 5.4μg/mL and it was inactive against normal cells.
  Fitoterapia 07/2011; 82(8):1169-74. · 1.85 Impact Factor