Bradd J Haley

Publications (17) View all

• Source

  Available from: Rita R Colwell

  Dataset: Haley et al 2012

  Bradd J Haley, Arlene Chen, Christopher J Grim, Philip Clark, Celia Municio Diaz, Elisa Taviani, Nur A Hasan, Elizabeth Sancomb, Wessam Mahmoud Elnemr, Muhammad A Islam, Anwar Huq, Rita R Colwell, Eva Benediktsdóttir
• Source

  Available from: Nur A Hasan

  Dataset: PNAS-2010-Hasan-21134-9: Comparative Genomics of Clinical and Environmental Vibrio mimicus

  Nur A Hasan, Christopher J Grim, Bradd J Haley, Jongsik Chun, Munirul Alam, Elisa Taviani, Mozammel Hoq, A Christine Munk, Elizabeth Saunders, Thomas S Brettin, David C Bruce, Jean F Challacombe, J Chris Detter, Cliff S Han, Gary Xie, G Balakrish Nair, Anwar Huq, Rita R Colwell
• Source

  Available from: Nur A Hasan

  Article: Vibrio cholerae in an Historically Cholera-Free Country.

  Bradd J Haley, Arlene Chen, Christopher J Grim, Philip Clark, Celia Municio Diaz, Elisa Taviani, Nur A Hasan, Elizabeth Sancomb, Wessam Mahmoud Elnemr, Muhammad A Islam, Anwar Huq, Rita R Colwell, Eva Benediktsdóttir
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  ABSTRACT: We report the autochthonous existence of Vibrio cholerae in coastal waters of Iceland, a geothermally active country where cholera is absent and has never been reported. Seawater, mussel, and macroalgae samples were collected close to and distant from sites where geothermal activity causes a significant increase in water temperature during low tides. V. cholerae was detected only at geothermal-influenced sites during low-tides. None of the V. cholerae isolates encoded cholera toxin (ctxAB) and all were non-O1/non-O139 serogroups. However, all isolates encoded other virulence factors that are associated with cholera as well as extra-intestinal V. cholerae infections. The virulence factors were functional at temperatures of coastal waters of Iceland, suggesting an ecological role. It is noteworthy that V. cholerae was isolated from samples collected at sites distant from anthropogenic influence, supporting the conclusion that V. cholerae is autochthonous to the aquatic environment of Iceland.
  Environmental Microbiology Reports 08/2012; 4(4):381-389. · 3.23 Impact Factor
• Source

  Available from: Rita R Colwell

  Article: Detection, isolation, and identification of Vibrio cholerae from the environment.

  Anwar Huq, Bradd J Haley, Elisa Taviani, Arlene Chen, Nur A Hasan, Rita R Colwell
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  ABSTRACT: Recent molecular advances in microbiology have greatly improved the detection of bacterial pathogens in the environment. These improvements and a downward trend in the cost of molecular detection methods have contributed to increased frequency of detection of pathogenic microorganisms where traditional culture-based detection methods have failed. Culture methods also have been greatly improved, and the confluence of the two suites of methods provides a powerful tool for detection, isolation, and characterization of pathogens. While molecular detection provides data on the presence and type of pathogens, culturing methods allow a researcher to preserve the organism of interest for "-omics" studies, such as genomic, metabolomic, secretomic, and transcriptomic analysis, which are rapidly becoming more affordable. This has yielded a clearer understanding of the ecology and epidemiology of microorganisms that cause disease. In this unit, we present commonly accepted methods for isolation, detection, and characterization of V. cholerae, providing more extensive knowledge of the ecology and epidemiology of this organism. This unit has been fully revised and updated from the earlier version with the latest knowledge and additional information not previously included.
  Current protocols in microbiology 08/2012; Chapter 6:Unit6A.5.
• Source

  Available from: Nur A Hasan

  Article: Genomic diversity of 2010 Haitian cholera outbreak strains.

  Nur A Hasan, Seon Young Choi, Mark Eppinger, Philip W Clark, Arlene Chen, Munirul Alam, Bradd J Haley, Elisa Taviani, Erin Hine, Qi Su, [......], Joseph B Prosper, Keziah Furth, M M Hoq, Huai Li, Claire M Fraser-Liggett, Alejandro Cravioto, Anwar Huq, Jacques Ravel, Thomas A Cebula, Rita R Colwell
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  ABSTRACT: The millions of deaths from cholera during the past 200 y, coupled with the morbidity and mortality of cholera in Haiti since October 2010, are grim reminders that Vibrio cholerae, the etiologic agent of cholera, remains a scourge. We report the isolation of both V. cholerae O1 and non-O1/O139 early in the Haiti cholera epidemic from samples collected from victims in 18 towns across eight Arrondissements of Haiti. The results showed two distinct populations of V. cholerae coexisted in Haiti early in the epidemic. As non-O1/O139 V. cholerae was the sole pathogen isolated from 21% of the clinical specimens, its role in this epidemic, either alone or in concert with V. cholerae O1, cannot be dismissed. A genomic approach was used to examine similarities and differences among the Haitian V. cholerae O1 and V. cholerae non-O1/O139 strains. A total of 47 V. cholerae O1 and 29 V. cholerae non-O1/O139 isolates from patients and the environment were sequenced. Comparative genome analyses of the 76 genomes and eight reference strains of V. cholerae isolated in concurrent epidemics outside Haiti and 27 V. cholerae genomes available in the public database demonstrated substantial diversity of V. cholerae and ongoing flux within its genome.
  Proceedings of the National Academy of Sciences 06/2012; 109(29):E2010-7. · 9.68 Impact Factor