Birgit Seisser

Publications (7) View all

• Chapter: Novel Developments Employing Redox Enzymes: Old Enzymes in New Clothes

  Kurt Faber, Silvia M. Glueck, Birgit Seisser, Wolfgang Kroutil
  05/2011: pages 199 - 249; , ISBN: 9780470627303
• Article: Cutting Long Syntheses Short: Access to Non‐Natural Tyrosine Derivatives Employing an Engineered Tyrosine Phenol Lyase

  Birgit Seisser, René Zinkl, Karl Gruber, Franz Kaufmann, Andreas Hafner, Wolfgang Kroutil
  Advanced Synthesis & Catalysis 03/2010; 352(4):731 - 736. · 6.05 Impact Factor
• Article: Synthesis of Optically Active Amines Employing Recombinant ω‐Transaminases in E. coli Cells

  Dominik Koszelewski, Madeleine Göritzer, Dorina Clay, Birgit Seisser, Wolfgang Kroutil
  ChemCatChem 11/2009; 2(1):73 - 77. · 5.21 Impact Factor
• Article: Shifting the equilibrium of a biocatalytic cascade synthesis to enantiopure epoxides using anion exchangers

  Joerg H Schrittwieser, Iván Lavandera, Birgit Seisser, Barbara Mautner, Jeffrey H Lutje Spelberg, Wolfgang Kroutil
  [show abstract] [hide abstract]
  ABSTRACT: Hydroxide-loaded anion exchangers have been successfully employed to shift the equilibrium of a one-pot, two-step, two-enzyme cascade reaction affording enantiopure epoxides starting from prochiral α-chloroketones. The α-chloroketones were asymmetrically reduced employing an alcohol dehydrogenase and then transformed further to the corresponding epoxides employing halohydrin dehalogenases. Each epoxide enantiomer could be obtained with up to 93% conversion in enantiomerically pure form (>99% ee). In contrast to previous studies the amount of hydride donor (2-propanol) could be reduced due to favoured halohydrin formation in the reduction of α-chloroketones.
  Tetrahedron Asymmetry 01/2009; 20:483-488. · 2.65 Impact Factor
• Article: Biocatalytic cascade for the synthesis of enantiopure β-azidoalcohols and β-hydroxynitriles

  Joerg H Schrittwieser, Iván Lavandera, Birgit Seisser, Barbara Mautner, Wolfgang Kroutil
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  ABSTRACT: A three-step, two-enzyme, one-pot reaction sequence starting from prochiral α-chloroketones leading to enantiopure β- azidoalcohols and β-hydroxynitriles is described. Asymmetric bioreduction of α-chloroketones by hydrogen transfer catalysed by an alcohol dehydrogenase (ADH) established the stereogenic centre in the first step to furnish enantiopure chlorohydrin intermediates. Subsequent biocatalysed ring closure to the epoxide and nucleophilic ring opening with azide, N3-, or cyanide, CN-, both catalysed by a nonselective halohydrin dehalogenase (Hhe) proceeded with full retention of configuration to give enantiopure β-azidoalcohols and β-hydroxynitriles, respectively. Both enantiomers of various optically pure β-azidoalcohols and β-hydroxynitriles were synthesised.
  European Journal of Organic Chemistry 01/2009; · 3.33 Impact Factor