Article: Biomarkers of lead exposure in petrol station attendants and auto-mechanics in Abeokuta, Nigeria: effect of 2-week ascorbic acid supplementation.[show abstract] [hide abstract]
ABSTRACT: In order to study the ameliorative effects of ascorbic acid in chronic lead poisoning, petrol station attendants and auto-mechanics in Abeokuta, Nigeria, who have been shown to be occupationally exposed to lead and university students (serving as control), were supplemented daily with 500mg ascorbic acid for 2 weeks. Blood and urine samples were collected from the subjects before and after ascorbic acid regimen and analysed for lead and biochemical effects associated with lead toxicity. The 2-week ascorbic acid supplementation resulted in a significant (P < 0.05) reduction in blood lead in the occupationally exposed subjects. The reduction in blood lead amounted to 57% in male petrol station attendants, 50% in female petrol station attendants and 44% in the auto-mechanics. Urinary excretion of lead increased remarkably in the occupationally exposed subjects (P < 0.05). The biochemical effects associated with the toxic effects of lead also responded positively to the ascorbic acid regimen. Plasma and urine aminolevulinic acid (ALA) were reduced significantly (P < 0.05) by as much as 55% and 57% respectively. Plasma calcium also increased significantly (P < 0.05) in the subjects. Decreased levels of reduced glutathione (GSH) and hemoglobin observed in the occupationally exposed subjects were reversed by ascorbic acid. Glutathione-S-transferase (GST) and catalase (CAT) activities were not affected. Our findings indicate that ascorbic acid may be useful as an economical and convenient prophylactic agent for lead poisoning.Environmental Toxicology and Pharmacology 07/2004; 17(3):169-76. · 1.47 Impact Factor
Article: Erythrocyte acetylcholinesterase activity as a surrogate indicator of lead-induced neurotoxicity in occupational lead exposure in Abeokuta, Nigeria.[show abstract] [hide abstract]
ABSTRACT: Dose-effect and dose-response relationships in occupational neurotoxicology are rarely studied by means of biochemical methods. In order to investigate the potential neurotoxic effects of lead during occupational exposure to this metal, the activity of erythrocyte acetylcholinesterase (AcChE), as well as blood pressure and pulse, were determined in various artisans in Abeokuta, Nigeria, who have been shown to be occupationally exposed to lead, and these were related to blood lead levels. AcChE activity in the artisans was inhibited to varying extents. While AcChE activity was inhibited to the tune of 39% in the male petrol station attendants, the inhibition amounted to 32% in female petrol station attendants. In other artisans, AcChE inhibition ranged from 31% in the welders to 38% in painters. The lowest inhibition of 15% was obtained in the panel beaters. Correlations, as calculated by Pearson's method, revealed a significant (p<0.001) inverse linear relationship between AcChE activity and blood lead levels (r=-0.40; y=-120.38x+13935.59; p<0.001). Blood pressure and pulse were not significantly different between control and lead-exposed subjects. Our findings suggest that erythrocyte AcChE activity could be used as a biomarker of lead-induced neurotoxicity in occupationally exposed subjects.Environmental Toxicology and Pharmacology 09/2007; 24(2):183-8. · 1.47 Impact Factor
Article: Biomarkers of lead exposure in petrol station attendants and auto-mechanics in Abeokuta, Nigeria: effect of 2-week ascorbic acid supplemention.Environ. Toxicol. Pharmacol. 01/2004; 17:169-176.