Axel Iván Villalobos Cortés |
|
M.V., M.Sc, Ph.D
|
|
Instituto de Investigación Agropecuaria de Panamá
·
Biotecnologia
|
Other
-
Journal RefereesArchivos de Zootecnia
Questions and Answers (8) View all
-
Answer added in MEGA22 Has anyone used MEGA software in phylogenetic studies?By Buhara Yucesan · Abant İzzet Baysal ÜniversitesiAxel Villalobos Cortés · Instituto de Investigación Agropecuaria de PanamáHI, MEGA is one of the most common softwares, friendly and easy to useHI, MEGA is one of the most common softwares, friendly and easy to useFollowing
-
Answer added in RAPD10 How to draw an RAPD dendogram?By Surjit Singh · Vellore Institute of TechnologyAxel Villalobos Cortés · Instituto de Investigación Agropecuaria de PanamáI am agree with Siva, the RAPD data analysis generally is in the form of bands in agarose gels. hence first convert that into binary score then you c... [more]I am agree with Siva, the RAPD data analysis generally is in the form of bands in agarose gels. hence first convert that into binary score then you can use some of the softwares above mentionedFollowing
-
Answer added in RAPD10 How to draw an RAPD dendogram?By Surjit Singh · Vellore Institute of TechnologyAxel Villalobos Cortés · Instituto de Investigación Agropecuaria de PanamáYou can draw a dendrogram with PHYLIP, TFPGA, POPGENE or SPLITS TREEYou can draw a dendrogram with PHYLIP, TFPGA, POPGENE or SPLITS TREEFollowing
-
Answer added in Dental13 Does anywone know how to extract the DNA from saliva ( I'm gonna use IQ KIt promega).By Varsik Hakopian · Université Libre de BruxellesAxel Villalobos Cortés · Instituto de Investigación Agropecuaria de Panamáany time Varsik!any time Varsik!Following
-
Answer added in Dental13 Does anywone know how to extract the DNA from saliva ( I'm gonna use IQ KIt promega).By Varsik Hakopian · Université Libre de BruxellesAxel Villalobos Cortés · Instituto de Investigación Agropecuaria de PanamáYou can use BuccalAmp DNA extraction solution kit http://www.epibio.com/You can use BuccalAmp DNA extraction solution kit http://www.epibio.com/Following
Publications (15) View all
-
Article: HISTORIA DE LOS BOVINOS EN PANAMÁ Y SU RELACIÓN CON LAS POBLACIONES BOVINAS DE IBEROAMÉRICA HISTORY OF PANAMA BOVINES AND THEIR RELATIONSHIPS WITH OTHER IBEROAMERICAN POPULATIONS
A.I. Villalobos-Cortés, A.M. MArtinez, J.V. Delgado[show abstract] [hide abstract]
ABSTRACT: Razas criollas. Conservación. Recursos zooge-néticos. RESUMEN Se hace una revisión histórica sobre el origen de la ganadería en América; se describen las migraciones de bovinos que se realizan desde España hacia las islas y los primeros envíos de animales desde La Española y Santiago hacia tierra firme, como Santa Marta, Colombia; Tamaulipas, México y Santa María La Antigua, Panamá. Se relata el movimiento de animales por todo el territorio panameño, influenciado por la presencia de Pedrarias Dávila como Gobernador de Castilla del Oro y posteriormente como Gober-nador de Nicaragua; se plantea la importancia del paso de bovinos por Panamá y su importancia en la conquista del Perú y Ecuador. Y se concluye con la hipótesis histórica de la influencia genética de los bovinos llegados al Nuevo Mundo sobre el territorio Iberoamericano particularmente los bovi-nos que llegaron a Panamá. SUMMARY An historic review of the origin of the American livestock is made; a description of the bovine migrations from Spain to the islands and the first sending of animals from La Española and Santiago to continental lands, like Santa Marta, Colombia; Tamaulipas, México and Santa María La Antigua, Panamá. The movement of animals over the Panamanian territory is related as influenced by Pedrarias Dávila, Governor of Castilla del Oro, later Governor of Nicaragua. The importance ofArchivos de Zootecnia 01/2009; 58:121--129. -
SourceAvailable from: Axel Iván Villalobos Cortés
Article: ESTRUCTURA GENÉTICA Y CUELLO DE BOTELLA DE LA POBLACIÓN BOVINA GUAYMÍ MEDIANTE MICROSATÉLITES GENETIC STRUCTURE AND BOTTLENECK OF THE GUAYMI BOVINE POPULATION BY MEANS OF MICROSATELLITES
Villalobos Cortés, Y Delgado, Criollo Conservación[show abstract] [hide abstract]
ABSTRACT: RESUMEN Se caracterizó la población bovina Guaymí con un panel de 27 microsatélites seleccionados a partir de las recomendaciones hechas por la FAO/ISAG. Se analizaron muestras aleatorias de ADN obtenidas de la población bovina criolla Guaymí que se encuentran ubicadas en el área que comprende la comarca indígena Ngöbe-Buglé en la zona montañosa de la provincia de Chiriquí en la República de Panamá. Para cada microsatélite se calcularon el contenido de información polimórfica (PIC), el número medio de alelos (Na), la heterocigosis observada (Ho), la heterocigosis esperada (He), el estadístico F IS , y equilibrio Hardy-Weinberg (HWE). Además se calculó si existía cuello de botella en esta población. Los valores obtenidos fueron: PIC: 0,6899; Ne: 4,04; He: 0,7243; Ho: 0,7088; F IS : 0,0356. Se observaron tres microsatélites en desequilibrio (p<0,05). Los valo-res encontrados se consideran dentro de los rangos obtenidos en otras poblaciones criollas, exóticas y autóctonas españolas. Se requiere realizar estudios más detallados de estas pobla-ciones y su relación con otras poblaciones bovi-nas. No se observa cuello de botella en el pasado reciente de esta población. SUMMARY Guaymi creole cattle were characterized by a 27 microsatellite panel, selected from aArchivos de Zootecnia 01/2011; 60:767--775. -
SourceAvailable from: Axel Iván Villalobos Cortés
Article: CARACTERIZACIÓN GENÉTICA DE LA POBLACIÓN BOVINA GUABALÁ MEDIANTE MICROSATÉLITES GENETIC CHARACTERIZATION OF THE GUABALA BOVINE POPULATION WITH MICROSATELLITES
[show abstract] [hide abstract]
ABSTRACT: RESUMEN Se caracterizó la población bovina Guabalá con un panel de 27 microsatélites seleccionados a partir de las recomendaciones de la FAO/ISAG (Food and Agriculture Organization/International Society of Animal Genetics) para estudios de biodiversidad genética bovina (FAO, 2004). Se analizaron muestras de ADN obtenidas de las poblaciones bovinas criollas Guabalá en la región Occidental de la República de Panamá y en la región del Valle de Antón, sitios donde se han ubicado ejemplares puros. La amplificación se realizó mediante la reacción en cadena de la polimerasa (PCR). La electroforesis se llevó a cabo mediante un secuenciador automático ABI PRISM 377 XL. La tipificación alélica se realizó con los paquetes informáticos Genescan v.3.2.3 y Genotyper v.3.7. Para cada microsatélite se cal-culó el contenido de información polimórfica (PIC), el número medio de alelos (Na), la heterocigosis observada (Ho), la heterocigosis esperada (He), el estadístico Fis, y equilibrio Hardy-Weinberg (HWE). Los valores obtenidos fueron: PIC: 0,6044; Na: 5,63; He: 0,6458; Ho: 0,6265; Fis: 0,0504. Se observó que 9 microsatélites estaban en desequi-librio (p<0,05). Los valores se pueden considerar similares a los encontrados en otras poblaciones bovinas autóctonas españolas y permitirán reali-zar estudios minuciosos y analizar las relaciones de esta población con otras poblaciones bovinas. SUMMARYArchivos de Zootecnia 01/2009; 58:485--488. -
Article: Genetic characterization of Latin-American Creole cattle usingmicrosatellite markers
J. V. Delgado, A. M. Martınez, A. Acosta, L. A. Alvarez, E. Armstrong, E. Camacho, J. Canon, O. Cortes, S. Dunner, V. Landi, [......], C. Rodellar, P. Sponenberg, O. Uffo, R. Ulloa-Arvizu, J. L. Vega-Pla, A.I. Villalobos-Cortes, D. Zambrano, P. Zaragoza, L. T. Gama, C. Ginja[show abstract] [hide abstract]
ABSTRACT: Genetic diversity in and relationships among 26 Creole cattle breeds from 10 American countries were assessed using 19 microsatellites. Heterozygosities, F-statistics estimates, genetic distances, multivariate analyses and assignment tests were performed. The levels of within-breed diversity detected in Creole cattle were considerable and higher than those previously reported for European breeds, but similar to those found in other Latin American breeds. Differences among breeds accounted for 8.4% of the total genetic variability. Most breeds clustered separately when the number of pre-defined populations was 21 (the most probable K value), with the exception of some closely related breeds that shared the same cluster and others that were admixed. Despite the high genetic diversity detected, significant inbreeding was also observed within some breeds, and heterozygote excess was detected in others. These results indicate that Creoles represent important reservoirs of cattle genetic diversity and that appropriate conservation measures should be implemented for these native breeds in order to minimize inbreeding and uncontrolled crossbreedingAnimal Genetics 02/2011; · 2.40 Impact Factor -
SourceAvailable from: Axel Iván Villalobos Cortés
Article: Genetic footprints of iberian cattle in america 500 years after the arrival of columbus.
Amparo M Martínez, Luis T Gama, Javier Cañón, Catarina Ginja, Juan V Delgado, Susana Dunner, Vincenzo Landi, Inmaculada Martín-Burriel, M Cecilia T Penedo, Clementina Rodellar, [......], Lilia Melucci, Guillermo Martínez-Velázquez, Jaime E Muñoz, Alicia Postiglioni, Jorge Quiroz, Philip Sponenberg, Odalys Uffo, Axel Villalobos, Delsito Zambrano, Pilar Zaragoza[show abstract] [hide abstract]
ABSTRACT: American Creole cattle presumably descend from animals imported from the Iberian Peninsula during the period of colonization and settlement, through different migration routes, and may have also suffered the influence of cattle directly imported from Africa. The introduction of European cattle, which began in the 18th century, and later of Zebu from India, has threatened the survival of Creole populations, some of which have nearly disappeared or were admixed with exotic breeds. Assessment of the genetic status of Creole cattle is essential for the establishment of conservation programs of these historical resources. We sampled 27 Creole populations, 39 Iberian, 9 European and 6 Zebu breeds. We used microsatellite markers to assess the origins of Creole cattle, and to investigate the influence of different breeds on their genetic make-up. The major ancestral contributions are from breeds of southern Spain and Portugal, in agreement with the historical ports of departure of ships sailing towards the Western Hemisphere. This Iberian contribution to Creoles may also include some African influence, given the influential role that African cattle have had in the development of Iberian breeds, but the possibility of a direct influence on Creoles of African cattle imported to America can not be discarded. In addition to the Iberian influence, the admixture with other European breeds was minor. The Creoles from tropical areas, especially those from the Caribbean, show clear signs of admixture with Zebu. Nearly five centuries since cattle were first brought to the Americas, Creoles still show a strong and predominant signature of their Iberian ancestors. Creole breeds differ widely from each other, both in genetic structure and influences from other breeds. Efforts are needed to avoid their extinction or further genetic erosion, which would compromise centuries of selective adaptation to a wide range of environmental conditions.PLoS ONE 01/2012; 7(11):e49066. · 4.09 Impact Factor