Atakan Sukatar

Botany,Marine Biology and Phycology
Prof.Dr.
19.34

Publications

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    Bülent Yorulmaz, Atakan Sukatar, Murat Barlas
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    ABSTRACT: Five biotic indices were used for the assessment of water quality of Eşen River in Southwest of Turkey. The classification of water quality based on benthic macroin-vertebrate and physical and chemical parameters were also done. Taxonomic composition of benthic macroinverte-brate fauna was used for calculation of the following biological indices; Saproby Index (SI), Biological Monitoring Working Party (BMWP), Average Score Per Taxon (ASPT), Family Biotic Index (FBI) and Belgian Biotic Index (BBI). Electrical conductivity, temperature, dissolved O 2 content, pH, BOD 5 , Cl-, NH 4-N, NO 2-N, NO 3-N, PO 4-P were analyzed. According to the data obtained, the water quality of Eşen River varied from poor to high ecological status. The indices SI, BMWP, BBI and ASPT were the more adequate estimate of water quality in accordance with physicochemical characteristics of the examined watercourse .
    Fresenius Environmental Bulletin 01/2015; · 0.53 Impact Factor
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    ABSTRACT: Cystoseira sp. is one of the most widely distributed genera of the Fucales order and provides an essential habitat for many epiphytes, invertebrates, and fish. Cystoseira sp. is found mostly in temperate regions of the Northern Hemisphere, such as the Mediterranean, Indian, and Pacific Oceans. Cystoseira sp. is characterized by highly differentiated basal and apical regions and presence of catenate pneumatocysts air-vesicles. Random Amplified Polymorphic DNA (RAPD-PCR) method is commonly used in toxicity, mutation detection and identification studies in macro and microalgae. Among PCR based studies it is widely recognised that RAPD-PCR is a rapid and reliable method for intra and inter-specific differentiation of most plant species. In this study RAPD method was used to determine genetic diversity between Cystoseria crinita (Desf.) Bory populations. 6 different populations of Cystoseria crinita (Desf.) Bory used as a material in the study. 11 different 10 bp RAPD-primers used and 8 of them gave acceptable bands. Some species identification keys for Cystoseira species are not generally give satisfying results. The Cystoseira species looks the same in some cases. Besides the morphological features are change according to seasons, enviromental conditions, life cycles and some enviromental effects. For these reasons molecular methods may be used to support morphological identification.
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    Ergun Taskin, Atakan Sukatar
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    ABSTRACT: PROOF © Published by Central Fisheries Research Institute (CFRI) Trabzon, Turkey in cooperation with Japan International Cooperation Agency (JICA), Japan The Red Algal Genera Laurencia, Osmundea and Palisada (Rhodomelaceae, Rhodophyta) in Turkey Introduction
    Turkish Journal of Fisheries and Aquatic Sciences 12/2013; 13(4):713-723. DOI:10.4194/1303-2712-v13_4_17 · 0.38 Impact Factor
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    ABSTRACT: The aim of the study was to evaluate the biological activities with toxic properties of the methanol, hexane, and chloroform extracts of Cystoseira compressa (Esper) Gerloff & Nizamuddin from the Coast of Urla in the Aegean Sea. The extracts of C. compressa were tested for their antimicrobial and antioxidant activities in this study. Cytotoxic and mutagenic potentials of the extracts were also evaluated using cell culture and mutagenicity assays. Hexane extract was found to have higher total flavonoid and phenolic contents than the other extracts and exerted higher antioxidant activity than other extracts. All extracts exhibited moderate antimicrobial activity against tested microorganisms (minimum inhibitory concentration ranges are 32-256 μg/mL). The results indicated that the extracts had no significant cytotoxic activity against human hepatocellular carcinoma Hep 3B cell line in all treated concentrations (5-50 μg/mL) and did not show mutagenicity in the Ames test. Lethality was not observed among mice treated with oral doses of the extracts. In conclusion, results of investigations indicate that brown alga C. compressa is a natural source of antioxidant. It has moderate antimicrobial activities with no toxicity.
    Cytotechnology 12/2013; 67(1). DOI:10.1007/s10616-013-9668-x · 1.45 Impact Factor
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    ABSTRACT: Diatoms represent an important class of aquatic phototrophs. They are not only one of the major contributors to global carbon fixation, but they also play a key role in the biogeochemical cycling of silica. Molecular identification methods based on conserved DNA sequences, such as internal transcribed spacer (ITS) have revolutionized our knowledge and understanding of conventional taxonomy. In this study, we aimed to compare the conventional identification methods with molecular identification methods. To do so, we isolated 4 diatom samples from the coast at Urla and characterized them Using light microscopy (LM) and scanning electron microscopy (SEM) according to morphological features. Then we amplified ITS regions using a conventional polymerase chain reaction (PCR), sequenced the PCR products, and analyzed the sequences using bioinformatic tools. Bioinformatic analysis indicated that the isolated species had high sequence similarity to Pseudo-nitzschia delicatissima, Achnanthes taeniata, Amphora coffeaeformis, and Cylindrotheca closterium. We think that molecular identification methods enable rapid and more reliable identification of diatom species and are crucial for monitoring harmful algal blooms.
    Turkish Journal of Biology 09/2013; 37:530-537. DOI:10.3906/biy-1205-40 · 1.22 Impact Factor
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    ABSTRACT: In the summer of 2010, the excessive growth of Cladophora laetevirens in the Southwestern shoreline of Istanbul, which carries great importance for tourism, drew major attention. The aim of this study was to investigate the excessive growth of Cladophora laetevirens, some ecological parameters, and the number of total coliform bacteria of surface waters in this region during the summer of 2010. During this period, this species multiplied and mucilage like environment was formed and the total coliform bacteria values were found to be between 105 and 108 MPN/100 mL. Due to the lack of available evidence prior to the sampling, it is possible that the excessive mucilage formation may have been trigged by Cladophora laetevirens. Cladophora samples were collected on different sampling dates during the 2010 summer (July and August). On these sampling days, the temperature, salinity and dissolved oxygen levels of the seawater ranged between 26.6-28°C, 14-33 ppt, and 8.2-9.7 mg/L, respectively. Nitrate+nitrite-N (0.21-0.26 μg-at/L), Ammonium-N (0.43-0.94 μg-at/L), Phosphate-P (0.53-3.50 µg-at/L), Silicate-Si (9.63-15.30 μg-at/L) and Chlorophyll a (3.68-14.11 µg/L) concentrations were measured. Marmara Denizi'nin Güneybatı İstanbul Sahilinde Cladophora laetevirens (Dillwyn) Kutzing ve enterik bakterilerin ani kitlesel artışı
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    ABSTRACT: ABSTRACT:The purpose of this project was to investigate the total protein, sugar and phenolic content in phosphate extracts of marine macroalgae species from the Aegean Sea. Colpomenia sinuosa (Mertens ex Roth) Derbès & Solier, Petalonia fascia (O.F. Müller) Kuntze, Scytosiphon lomentaria (Lyngbye) Link., Dilophus spiralis (Montagne) G. Hamel, and Padina pavonia J.V. Lamouroux were collected from Izmir Bay during April, as well as Cystoseira sp. collected on the Urla, Bodrum, Fethiye and Kas coastlines during the spring and summer months. The phenolic content of five brown algae extracts varied from 2.5 mg to 21.8 mg GAE/10 g. Dilophus spiralis extracts demonstrated the lowest concentrations based on total protein assay, while Scytosiphon lomentaria extracts showed the highest sugar contents. The extract of Cystoseira sp. collected from Fethiye and Kas, gave the highest concentrations in May.
    Botanica Marina 06/2012; 36(2):91-95. · 1.00 Impact Factor
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    Current Opinion in Biotechnology 09/2011; 22. DOI:10.1016/j.copbio.2011.05.467 · 8.04 Impact Factor
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    ABSTRACT: The biological activities of Laurencia obtusa and Laurencia obtusa var. pyramidata from Cesme coasts (Turkey) were screened by using in vitro methods. The essential oils of L. obtusa and L. obtusa var. pyramidata were obtained by steam distillation and their chemical compositions were analyzed by GC/MS. The antimicrobial activities against bacteria (two specific pathogenic strains (methicillin-oxacillin resistant Staphylococcus aureus ATCC 43300, hemorrhagic Escherichia coli (O157: H7) RSSK 232)) and one yeast strain (Candida albicans ATCC 10239) were determined by using the disc-diffusion technique. The antimicrobial potential of the L. obtusa essential oil was found considerably strong. It showed inhibitory effect on two bacteria and one yeast strain. The hexane and chloroform extracts of L. obtusa and the methanol and chloroform extracts of L. obtusa var. pyramidata were generally found as moderate antioxidants in comparison with butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA) and α-tocopherol (Vitamin E). The chloroform extracts of both the red algae have high phenolic content compared to the other extracts and essential oils.
  • Dilek Unal, Nuray O. Isik, Atakan Sukatar
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    ABSTRACT: In order to contribute to understanding of the response to metal stress, Ramalina farinacea (L.) Ach. was treated with different concentrations of chromium (VI) (5, 15, and 30 mM of K2CrO4) for 1, 3, and 24 h, and alterations in the photosystem II photosynthetic quantum yield, pigment content, integrity of chlorophyll, cell viability, and proline accumulation were investigated. Significant alterations of the photosynthetic quantum yield (F v/F m) ratio were observed in response to the increase in chromium concentration. The F v/F m ratio decreased in R. farinacea following 24-h treatment with 30 mM Cr6+ solution. In present study, in both control and other plant groups treated with 5 mM Cr6+, the Chl a/b ratio was approximately within the range of 2.0–3.5. However, this ratio significantly decreased for the samples treated with 15 (exposure period of 24 h) and 30 mM; (exposure periods of 3 and 24 h) Cr6+. We also showed that cell viability of samples treated with 15 and 30 mM Cr6+ significantly decreased. Accumulation of metal resulted in proline accumulation in R. farinacea thalli; however, when photodestructive effects on photosystem II occurred, proline intracellular concentration declined. On the basis of these results, we suggest that proline accumulation might not be the stress marker during heavy metal stress. Key words Ramalina farinacea -chromium (VI)-photosynthesis-proline accumulation-lichen
    Russian Journal of Plant Physiology 09/2010; 57(5):664-669. DOI:10.1134/S1021443710050092 · 0.76 Impact Factor
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    Inci Tuney, Atakan Sukatar
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    ABSTRACT: There are various methods published about DNA extraction from marine algae. These methods are the modifications of several DNA extraction methods from other organisms. Extraction of DNA from seaweeds are difficult processes because of the polysaccharide and polyphenole compounds of their thallus. In this study, DNA is extracted from a brown alga (Scytosiphon lomentaria and Cystoseira sp., Ectocarpus sp. ) collected from the Bay of Izmir by using modified CTAB (cetiltrimethylamonium bromide) protocol and used in PCR analysis. This modified method was also found efficient and applicaple for other molecular purposes.
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    Koçak, Katağan, Sukatar
    Zoology in the Middle East 01/2010; 49:118-120. DOI:10.1080/09397140.2010.10638403 · 0.52 Impact Factor
  • Esra Ersoy Omeroglu, Ismail Karaboz1 and Atakan Sukatar
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    ABSTRACT: Some Vibrio strains are pathogenic and cause vibriosis, a serious infectious disease in maricultured organisms, and in recent years, Vibrio harveyi has been described to be pathogenic to children with cancer. Bioluminescent V. harveyi TEMO5 and TEMS1 were isolated from intestine contents of Holothuria tubulosa and seawater of Izmir Gulf (Turkey) for the first time. Hence, in this study, susceptibility patterns of 21 different antibiotics against these isolates were investigated by agar disc diffusion and micro-dilution method. The findings indicate that while these isolates were resistant to β-lactam antibiotics (ampicillin, cephalothin and cephazolin) and very sensitive to aminoglycoside antibiotics (tobramycin and gentamicin), sulphamethoxazole/ trimethoprim and chloramphenicol, they were resistant to amoxicillin- clavulanic acid inhibiting β-lactamases. Therefore, V. harveyi TEMO5 and TEMS1 are likely to acquire resistance by maintaining in response to natural environmental selection, or changing the permeability of outer membrane anion (porin) channels, or at chromosomal level.
    Fresenius Environmental Bulletin 05/2009; 18(11):2023-2028. · 0.53 Impact Factor
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    ABSTRACT: Macroalgae are rich sources of natural bioactive products. Their secondary metabolites are attracted attends in many fields of life i.e., food, animal feeding, textile, cosmetics, medicine etc. In this study, methanol, dichloromethane and hexane extracts of Codium fragile (Chlorophyta) were tested for antimicrobial and antioxidant activities. Antioxidant effects were evaluated by hydroxyl radical scavenging assay (deoxyribose degradation assay) and b-carotene bleaching assay. The extracts indicated relatively little antioxidant activities, as compared to commercial antioxidants. The composition of the essential oil of C. fragile was also analyzed by GC and GC-MS. Twenty four compounds were identified of C. fragile essential oil and n-tricosane (11.88%) was determined as major component. Essential oil of C. fragile showed weak antibacterial activity against all gram positive bacteria tested, except methicillin-oxacillin resistant Staphylococcus aureus ATCC 43300. Similarly, all the extracts of C. fragile showed weak antimicrobial activity on tested organisms.
    Asian Journal of Chemistry 01/2009; 21(2):1197-1209. · 0.36 Impact Factor
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    ABSTRACT: The present study was conducted to evaluate the antioxidant and antimicrobial activity of methanol, dichloromethane and hexane extracts, as well as the essential oils of brown algae (Phaeophyta) Colpomenia sinuosa, Dictyota dichotoma, Dictyota dichotoma var. implexa, Petalonia fascia and Scytosiphon lomentaria. The essential oil of the macroalgae was obtained by steam distillation and analyzed by GC and GC/MS. The antioxidant activity of the algal extracts was determined using the procedures of inhibition of b-carotene bleaching and ABTS+ methods. The antioxidant effects of the extracts were compared with those of commercial antioxidants, such as butylated hydroxytoluene (BHT), butylated hydroxyanisol (BHA) and a-tocopherol. The hexane extracts of D. dichofoma var. implexa had a higher phenolic content than the other extracts. The dichloromethane extract of S. lomentaria was found to be more active in the decolorization of ABTS+ than the other extracts and generally the dichloromethane extracts were more active than the methanol and hexane extracts. Antimicrobial activities of the extracts were assessed against Gram (+) and Gram (–) bacteria and one yeast strain by the disk diffusion method. According to the re¬sults, the dichloromethane extracts generally showed more potent antimicro¬bial activity than the methanol and hexane extracts at concentrations 1.5 and 1.0 mg/disk.
    Journal of the Serbian Chemical Society 01/2009; 74(6):619-628. DOI:10.2298/JSC0906619D · 0.89 Impact Factor
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    ABSTRACT: In the present study, Physcia semipinnata samples were exposed to UV-A (352 nm) in 20 J m(-2) for 24 h, 48 h and 72 h to seek the alterations in the PSII photosynthetic quantum yield, thallus anatomy and DNA mutation rate in response to radiation. The Fv/Fm ratio decreased in P. semipinnata following exposure to UV-A for 24h, 48 h and 72 h. The data of the present study reported that Chla degradation occurred by exposition of UV-A for 24 h, 48 h and 72 h. Photobiont size and thickness of upper cortex layer also showed a gradually decrease in P. semipinnata thallus sections during 24 h, 48 h and 72 h. The thickness of the upper cortex layer, exposed to UV-A for 72 h, of P. semipinnata thallus reduced 64%, compared with control. The random amplified polymorphic DNA (RAPD) technique was used to detect DNA damage. The main changes observed in the RAPD profiles resulted in both appearance and disappearance of different bands and variation of their intensity. According to RAPD assay, the genetic distance between the control group and 24h , 48 h and 72 h UV-A exposed, groups was found 56%, 78% and 84%, respectively.
    Journal of Photochemistry and Photobiology B Biology 11/2008; 94(1):71-6. DOI:10.1016/j.jphotobiol.2008.10.004 · 2.80 Impact Factor
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    ABSTRACT: The methanol, dichloromethane, hexane, chloroform, and volatile components of Dictyopteris membranaceae. (Stackhouse) Batters (Dictyotaceae) and Cystoseira barbata. (Good et Woodw.) J. Agardh (Cystoseiraceae) were tested for their antimicrobial activities (four Gram-positive bacteria, four Gram-negative bacteria, and Candida albicans. ATCC 10239). Five compounds were identified in the volatile oil of D. membranaceae. accounting for >85% of the composition of the volatile oil. Twenty-eight compounds were identified in the volatile oil of C. barbata. accounting for >67% of the composition of the volatile oil. Major components were 6-butyl-1,4-cycloheptadiene (43.21%) for D. membranaceae. and docosane (7.61%) and tetratriacontane (7.47%) for C. barbata.. Many compounds in the volatile oil of C. barbata. were identified as hydrocarbon compounds. The volatile oils of these algae did not remarkably inhibit the growth of tested microorganisms. However, the hexane extracts showed more potent antimicrobial activity than methanol, dichloromethane, and chloroform extracts.
    Pharmaceutical Biology 10/2008; 44(3):183-188. DOI:10.1080/13880200600685949 · 1.34 Impact Factor
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    Dilek Unal, Inci Tuney, Atakan Sukatar
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    ABSTRACT: Physcia semipinnata was exposed to UV-A (352nm) and visible light (210, 800 and 2000mW/cm2) for 30min, 1, 2, 24, and 48h to seek the alterations in the PSII photosynthetic quantum yield, in response to radiation. Chlorophyll a fluorescence did not influence exposure to light, 210, 800 and 2000mW/cm2. Significant alterations of the photosynthetic quantum yield ratio occurred in response to increase in UV-A exposure time. The photosynthetic quantum yield ratio decreased in P. semipinnata following exposure to UV-A for 24 and 48h. The thalli of P. semipinnata treated with 1mM polyamine were not influenced during the exposure to UV-A for 24 and 48h. It was also found that exogenously spd added samples had higher chla content than spm and put added samples. In this study, we showed that lipid peroxidation levels between UV-A-treated samples and exogenously polyamine treated samples that were previously exposed to UV-A for 24 and 48h were significantly decreased. This result is the first record to indicate that external polyamines might have some protective role on photosystem II and membrane against UV-A stress.
    Journal of Photochemistry and Photobiology B Biology 02/2008; 90(1):64-8. DOI:10.1016/j.jphotobiol.2007.11.004 · 2.80 Impact Factor
  • D. Unal, A. Senkardesler, A. Sukatar
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    ABSTRACT: The levels of endogenous abscisic acid and total polyamine contents (putrescine and spermidine) were studied in two lichen species differing in desiccation tolerance. ABA and polyamine contents showed significant differences between air-dried and water-treated thalli of Pseudevernia furfuracea and Ramalina farinacea. The levels of putrescine (put) and spermidine (spd) in water-treated P. furfuracea thalli were found higher than in dry samples. The values for spd were almost threefold and for put almost 1.4-fold higher than in airdried samples. The levels of put and spd in water-treated R. farinacea were lower than in the air-dried samples. The lowest values for spd and put were almost 33-fold and 2.8-fold less, respectively. ABA contents of water-treated P. furfuracea and R. farinacea increased by 2.65% and 25.5% when compared with air-dried thallus. Key wordsPseudevernia furfuracea–Ramalina farinacea–abscisic acid–desiccation–lichens–polyamines–rehydration
    Russian Journal of Plant Physiology 01/2008; 55(1):115-118. DOI:10.1134/S1021443708010147 · 0.76 Impact Factor

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