Anthony P F Turner

Publications (179) View all

• Article: Disposable Screen-Printed Electrodes (Spe) Mercury-Free for Lead Detection

  Ilaria Palchetti, Christa Upjohn, A.P.F. Turner, M. Mascini
  10/2011;
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  Available from: Anthony P F Turner

  Article: Electrochemical Immunoassay for Free Prostate Specific Antigen (f‐PSA) Using Magnetic Beads

  P. Sarkar, D. Ghosh, D. Bhattacharyay, S. J. Setford, A. P. F. Turner
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  ABSTRACT: Prostate specific antigen (PSA) is a prominent marker for the prostate carcinoma. It is found in human blood in free (f-PSA) and complex forms. These two forms together are called total PSA (t-PSA). Estimation of both forms is essential to predict malignancy. In this study we report a unique and effective technique of electrochemical detection of f-PSA using magnetic beads on a three-electrode screen-printed sensor. A magnetic bead enzyme linked immunosorbent assay (ELISA) was performed in a cuvette. Following the immunoassay, magnetic beads were recovered by a magnetic concentrator and transferred on the working electrode of the 3-electrode assembly. The amperometric response, a measure of the amount of residual enzyme activity on the beads and hence the concentration of analyte in solution, was determined by addition of enzyme substrate. The device has a detection limit of <0.1 ng mL−1 f-PSA and a linear range of 0 to 1 ng mL−1 f-PSA.
  Electroanalysis 06/2008; 20(13):1414 - 1420. · 2.87 Impact Factor
• Article: Detection of pesticide by polymeric enzyme electrodes.

  K Dutta, D Bhattacharyay, A Mukherjee, S J Setford, A P F Turner, P Sarkar
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  ABSTRACT: Screen-printed electrodes (SPEs) containing immobilized acetylcholine esterase (AChE) enzyme were used for the electrochemical determination of organophosphorous (OP) and carbamate pesticides. The extent of AChE deactivation by the pesticide was determined in the presence of acetylcholine (AChCl) substrate. The unique nature of this approach lies in the enzyme immobilization procedure in which AChE was attached to the SPE by in situ bulk polymerization of acrylamide to ensure efficient adherence within the membrane with minimal losses in enzyme activity. Responses were observed for the pesticides Monocrotophos, Malathion, Metasystox and Lannate over the concentration range 0-10 ppb (microg L(-1)).
  Ecotoxicology and Environmental Safety 04/2008; 69(3):556-61. · 2.29 Impact Factor
• Article: Molecularly imprinted polymers for the recognition of proteins: the state of the art.

  A Bossi, F Bonini, A P F Turner, S A Piletsky
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  ABSTRACT: Molecular imprinting has proved to be an effective technique for the creation of recognition sites on a polymer scaffold. Protein imprinting has been a focus for many chemists working in the area of molecular recognition, since the creation of synthetic polymers that can specifically recognise proteins is a very challenging but potentially extremely rewarding objective. It is expected that molecularly imprinted polymers (MIPs) with specificity for proteins will find application in medicine, diagnostics, proteomics, environmental analysis, sensors and drug delivery. In this review, the authors provide an overview of the progress achieved in the decade between 1994 and 2005, with respect to the challenging area of MIPs for protein recognition. The discussion furnishes a comparative analysis of different approaches developed, underlining their relative advantages and disadvantages and highlighting trends and possible future directions.
  Biosensors and Bioelectronics 02/2007; 22(6):1131-7. · 5.60 Impact Factor
• Chapter: Biosensors and Biomimetic Sensors for the Detection of Drugs, Toxins and Biological Agents

  A. P. F. Turner, S. Piletsky
  07/2006: pages 261-272;