Questions and Answers (3) View all
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Answer added in Mass Spectrometry13 Can I identify peptides with N-linked glycosylation by ESI-QUAD-TOF?By Gustavo Moreira · Universidade Federal de PelotasAlexander Chizhov · Russian Academy of SciencesTo be correct, N- and O- chains have to be removed and analyzed separately. For methods, see e.g., papers of A. Dell and H. Morris Cancel or SaveTo be correct, N- and O- chains have to be removed and analyzed separately. For methods, see e.g., papers of A. Dell and H. Morris Cancel or SaveFollowing
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Answer added in Mass Spectrometry9 Is there anyone who knows a free program to transform Thermo Q-Exactive raw files in mzXML or mzML? Cheers, FrankBy Frank Schmidt · University of GreifswaldAlexander Chizhov · Russian Academy of SciencesTo be correct, N- and O- chains have to be removed and analyzed separately. For methods, see e.g., papers of A. Dell and H. MorrisTo be correct, N- and O- chains have to be removed and analyzed separately. For methods, see e.g., papers of A. Dell and H. MorrisFollowing
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Answer added in Mass Spectrometry5 Mass spec sample prep: Recommended pipettes, tips, and microfuge tubesBy Rich Beck · University of Minnesota Twin CitiesAlexander Chizhov · Russian Academy of SciencesUse Plastibrand centrifuge tubes (NEVER use Isolab tubes!), as for tips, Eppendorf is ok (low contaminations). Never use so-called "DNAase&RNAase-free... [more]Use Plastibrand centrifuge tubes (NEVER use Isolab tubes!), as for tips, Eppendorf is ok (low contaminations). Never use so-called "DNAase&RNAase-free tips". All polymer filters for blotting give huge contaminations.Following
Publications (22) View all
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Dataset: ja2083015 si 001
Alexander O Chizhov, Gabriele Loers, Ana Ardá, Melitta Schachner, Jesús Jiménez-Barbero, Yury E Tsvetkov, Elena V Sukhova, Elena A Khatuntseva, Nikolay E Nifantiev, Monika Burg-Roderfeld, Hans-Christian Siebert, Alexey A Grachev -
SourceAvailable from: Nikolay E Nifantiev
Dataset: b(1-6)-Cycloglucosamines - EurJOC 2010 - cover picture
Yury E. Tsvetkov, Alexey A. Grachev, Alexey G. Gerbst, Marina L. Gening, Olga N. Yudina, Alexander S. Shashkov, Denis V. Titov, Nikolay E. Nifantiev, Alexander O. Chizhov -
SourceAvailable from: Nikolay E Nifantiev
Dataset: ja2083015 si 002
Alexander O Chizhov, Gabriele Loers, Ana Ardá, Melitta Schachner, Jesús Jiménez-Barbero, Yury E Tsvetkov, Elena V Sukhova, Elena A Khatuntseva, Nikolay E Nifantiev, Monika Burg-Roderfeld, Hans-Christian Siebert, Alexey A Grachev -
Article: Structures of cell-wall phosphate-containing glycopolymers of Bifidobacterium longum BIM B-476-D.
Olga A Valueva, Alexander S Shashkov, Evelina L Zdorovenko, Alexander O Chizhov, Elena Kiseleva, Galina Novik, Yuriy A Knirel[show abstract] [hide abstract]
ABSTRACT: Glycopolymers with oligosaccharyl phosphate repeats of two types and ribitol and glycerol teichoic acids were isolated from cell wall of Bifidobacterium longum BIM B-476-D by stepwise extraction with 10% CCl3CO2H. The following structures of the glycopolymers were established by sugar analysis, selective cleavage with aq 2% HOAc, dephosphorylation with 48% HF, 2D NMR spectroscopy, and high-resolution ESI MS: The ribitol teichoic acid also contains minor d-alanine, whose position was not determined.Carbohydrate research 03/2013; 373C:22-27. · 2.03 Impact Factor -
Article: Synthesis of branched arabinofuranose pentasaccharide fragment of mycobacterial arabinans as 2-azidoethyl glycoside.
Ksenia G Fedina, Polina I Abronina, Nikita M Podvalnyy, Nikolay N Kondakov, Alexander O Chizhov, Vladimir I Torgov, Leonid O Kononov[show abstract] [hide abstract]
ABSTRACT: Branched arabinofuranose pentasaccharide with 2-azidoethyl aglycon was prepared for the first time by [3+1+1] bis-(1,2-cis)-glycosylation of trisaccharide diol with silyl-protected thioglycoside glycosyl donor. The presence of 2-azidoethyl aglycon would enable the preparation of neoglycoconjugates using the click chemistry approaches.Carbohydrate research 05/2012; 357:62-7. · 2.03 Impact Factor