Skills (2)
Research experience
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Aug 2010–
presentResearch: Imaging and quantifying membrane order in zebrafish
University of New South Wales · Centre for Vascular Research (CVR) · Membrane Biology GroupAustralia · Kensington
Education
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Aug 2010
University of New South Wales
PhDAustralia · Sydney -
Sep 2006–
Feb 2009Jordan University of Science & Technology
Diagneostic Molecular Biology & human genetics · MasterJordan · Irbid
Awards & achievements
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Aug 2010Scholarship: University International Postgraduate Award (UIPA)
Other
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LanguagesEnglish and Arabic
Questions and Answers (4) View all
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Answer added in Aldehydes65 GFP fluorescence after fixationwhy don't you try to wash with 0.1M Glycine for 15 min at RT, after fixation. Because Glycine is quenching the formaldehyde and terminates the cross-l... [more]
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Answer added in Microscopy3 Review this article it's very interested "Cell Lineage Reconstruction of Early Zebrafish Embryos Using Label-Free Nonlinear Microscopy"It's about implementing THG and SHG with 2PEF which produce very good images. You can see this link http://www.sciencemag.org/content/329/5994/967/su... [more]
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Question asked in Microscopy3 Review this article it's very interested "Cell Lineage Reconstruction of Early Zebrafish Embryos Using Label-Free Nonlinear Microscopy"!
Publications (2) View all
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Article: Characterization of a new series of fluorescent probes for imaging membrane order.
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ABSTRACT: Visualization and quantification of lipid order is an important tool in membrane biophysics and cell biology, but the availability of environmentally sensitive fluorescent membrane probes is limited. Here, we present the characterization of the novel fluorescent dyes PY3304, PY3174 and PY3184, whose fluorescence properties are sensitive to membrane lipid order. In artificial bilayers, the fluorescence emission spectra are red-shifted between the liquid-ordered and liquid-disordered phases. Using ratiometric imaging we demonstrate that the degree of membrane order can be quantitatively determined in artificial liposomes as well as live cells and intact, live zebrafish embryos. Finally, we show that the fluorescence lifetime of the dyes is also dependent on bilayer order. These probes expand the current palate of lipid order-sensing fluorophores affording greater flexibility in the excitation/emission wavelengths and possibly new opportunities in membrane biology.PLoS ONE 01/2013; 8(2):e52960. · 4.09 Impact Factor -
Article: Quantitative imaging of membrane lipid order in cells and organisms.
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ABSTRACT: It is now recognized that lipids and proteins in cellular membranes are not homogenously distributed. A high degree of membrane order is the biophysical hallmark of cholesterol-enriched lipid rafts, which may induce the lateral sorting of proteins within the membrane. Here we describe a quantitative fluorescence microscopy technique for imaging localized lipid environments and measuring membrane lipid order in live and fixed cells, as well as in intact tissues. The method is based on the spectral ratiometric imaging of the polarity-sensitive membrane dyes Laurdan and di-4-ANEPPDHQ. Laurdan typically requires multiphoton excitation, making it suitable for the imaging of tissues such as whole, living zebrafish embryos, whereas di-4-ANEPPDHQ imaging can be achieved with standard confocal microscopes. This approach, which takes around 4 h, directly examines the organization of cellular membranes and is distinct from alternative approaches that infer membrane order by measuring probe partitioning or dynamics.Nature Protocol 01/2011; 7(1):24-35. · 8.36 Impact Factor
