Article: Simple enrichment and independent cultures to expand bacterial community analysis from Gedongsongo hot spring.[show abstract] [hide abstract]
ABSTRACT: Denaturing gradient gel electrophoresis was used to identify bacterial communities from Gedongsongo hot spring, Indonesia, by culture-independent and -dependent procedures. Predominant species were found to be closely related to beta-proteobacteria, particularly from genus Ralstonia and Delftia. Other species present include alpha- and gamma-proteobacteria and Thermus groups. Some of the strains showed unique 16S rDNA sequences.Journal of Bioscience and Bioengineering 09/2008; 106(2):211-4. · 1.79 Impact Factor
Article: Culture-independent and culture-dependent approaches on microbial community analysis at Gedongsongo (GS-2) hot springInternational Journal of Integrative Biology. 01/2008;
Article: High-performance liquid chromatography (HPLC) nano analysis of antioxidant compounds of Iranian medicinal plants[show abstract] [hide abstract]
ABSTRACT: Four Iranian plants (pomegranate, khakshir, shevid, and chaye-shomal), already known to have various medicinal properties since several decades ago, were evaluated for their antioxidant compounds. Most of these bioactive properties correlating with antioxidant activity of phenolic compounds found in the plants. The high-performance liquid chromatography analysis was used in pomegranate juice and methanol extracts of the rest of the samples. Based on applied reference compounds which are gallic acid, vanilic acid, ascorbic acid, quercetin, caffeic acid, catechin and coumaric acid; khakshir and chaye-shomal demonstrated some high comparable peaks. Both pomegranate and shevid showed very low peaks in this area, indicating another phenolic compounds imposed by these plants.05/2012; 6:3459-3463.
Article: Nano study of antioxidant activities of fermented soy whey prepared with lactic acid bacteria and kefir[show abstract] [hide abstract]
ABSTRACT: This study aims were to test antioxidative activity of supernatant from fermented soy whey to find out their nutraceutical potential. Fermented soy whey was prepared using Lactobacillus plantarum, Streptococcus thermophilus and Kefir, fermented at room temperature (25-26°C) for 24 and 48 h. Antioxidative properties were assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity and reducing power. All fermented soy whey extracts exhibited a potentially antioxidant activities, yet extract kefir soy whey with 48 h incubation gave significant highest antioxidant activity compared to others. The results suggest that fermented soy whey formulations could provide a functional food alternative to milk-based fermented products.01/2012; 6:426-430.
Conference Proceeding: Ribotyping Based Analysis of Thermophilic Communities Using Culture Dependent Approach[show abstract] [hide abstract]
ABSTRACT: The bacterial community in Gedongsongo hot spring was analyzed by denaturing gradient gel electrophoresis (DGGE). The Gedongsongo hot spring has temperature at around 50°C and pH 4. Culture dependent community was studied through culturing the bacterial hot in four kind minimal medium. 16S ribosomal DNA fragments, including V4 -5 regions, were amplified with universal primers and analyzed by DGGE. The microbial communities of thermophiles culture using Nutrient Broth (aerobic) were dominated by genus Alicyclobacillus. While the uncultured bacteria from genus Proteobacteria were dominant in Nutrient Broth (anaerobic) and Yeast-Tryptone (aerobic) cultures. Introduction The realization that less than 1% of naturally microorganism can be isolated and grown in pure culture has creating great interest in developing of methods to get access to uncultivable microbes (Amann et al. 1995). Many of these unculturable microorganisms represent new phylotypes, families and divisions in domain bacteria and archaea. 'Unculturable' bacterial diversity presents a vast gene pool for biotechnological exploitation and poses a major challenge for microbiologists to understand their phylogenetic relationship and ecological significance (Sharma et al., 2005). This culturable proportion is not representative of the total phylogenetic diversity, because the members of 11 of 39 bacterial divisions have not been cultured yet (Leadbetter, J. R. 2003). The sequence analysis of gene encoding small-subunit ribosomal RNA (16S rRNA) is currently the most promising approach for the phylogenetic classification of bacteria. Furthermore, the comparative analysis of 16S rRNA gene sequences is a new means to investigate discrepancies between strain collections and natural communities (Ferris et al. 1996). Sequences of 16S rRNA genes can be retrieved by PCR from small amounts of DNA extracted either from laboratory cultures or natural environments, without being dependent to cultivation or growth conditions (Muyzer, 1999). The combination of PCR and denaturing gradient gel electrophoresis (DGGE), a technique for the sequence dependent separation of DNA molecules has proved to be useful technique (Muyzer et al., 1993). This technique could visualize the diversity of cyanobacterial 16S rRNA genes in environmental samples, to detect the uniqueness of isolated strains, and to assign PCR products derived from cultures to populations in the field. PCR products containing a single homogeneous population of DNA molecules can be recognized as a single band after DGGE and can be directly sequenced. Sequence data, therefore, can be generated without the time-consuming techniques required for molecular cloning procedures from bacterial cultures containing heterotrophic bacteria, thereby allowing the rapid survey of a collection of strains for genetic diversity (Nubet et al. 1997). Our objective in this research was to describe the bacterial community of thermophiles from Gedongsongo hot spring. This study was carried out using culture dependent approach.International Conference and Workshop on Basic and Applied Sciences / UNAIR-RuG-KNAW-UTM, Surabaya, Indonesia; 01/2007