Abhishek Sharma

Publications (5) View all

• Article: Detection Of Heavy Metals From Leaf Powders Of Annona Squamosa Linn., Datura Metel Linn. And Vitex Negundo Linn. Using ICP- OES Technique

  Abhishek L.Sharma, Sunita Shailajan
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  ABSTRACT: Heavy metal content in leaf powders of Annona squamosa Linn., Datura metel Linn., Vitex negundo Linn. was analyzed with the help of ‘Inductively Coupled Plasma- Optical Emission Spectroscopy’ as a safety measure of the plants for use in a polyherbal combination. The above mentioned three plants have insecticidal properties and they are used against head lice. The heavy metals determined in three leaf powders were Cu, Pb, Hg, Ni and Zn. Mercury was not detected in any of the three leaf powders. Concentration of Nickel exceeded the normal range in Annona squamosa Linn. (6.05 ppm), Datura metel Linn. (22.86 ppm), and Vitex negundo Linn. (6.92 ppm). The concentrations of Copper were within the range in Annona squamosa Linn. (10.34 ppm) and Vitex negundo Linn. (9.77 ppm), while it was maximum in Datura metel Linn. (25.07 ppm). Lead concentration was within the normal range in leaf powders of Annona squamosa Linn. (6.82 ppm) and Vitex negundo Linn. (5.75 ppm) while it was found to maximum in leaf powder of Datura metel Linn. (20.40 ppm). Zinc concentration was within the normal range in all the three plants and they ranged 12.17 ppm in Annona squamosa Linn., 53.09 ppm in Datura metel Linn. and 26.52 ppm in Vitex negundo Linn.
  Environmental Science- An INDIAN JOURNAL, Trade Science. 04/2009; 4:1- 4.
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  Article: Quantification of Swertiamarin from Whole Plant Powder of Swertia densiflora (Griscb.) Kashyap. Collected in Different Seasons

  Sunita Shailajan, Abhishek Sharma
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  ABSTRACT: In this paper, the comparative quantification of Swertiamarin from Swertia densiflora (Griscb.) Kashyap in different periods, in order to identify the best period for harvest is investigated. The plant powder was first extracted with methanol and then in chloroform. The final residue was reconstituted in methanol and used for quantitation. Chromatography was performed on silica gel 60 F254 HPTLC plate, with ethyl acetate:methanol:water, 7.5:1.5:1.2 (v/v), as mobile phase. Quantitation was achieved by densitometric scanning at 244 nm (λmax) in reflectanceabsorbance mode. The response to swertiamarin was linear function of concentration over the range 30 to 100 μL mL-1 in the extract of Swertia densiflora (Griscb.) Kashyap. The amount of swertiamarin in Swertia densiflora (Griscb.) Kashyap. whole plant powder was found to be 2.94 mg g-1 in the month of March, 1.59 mg g-1 in October and 1.18 mg g-1 in December.
  Asian Journal of Chemistry. 03/2009; 21:4351-4356.
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  Thesis: Evaluation of plants with insecticidal properties for use as antilice formulation

  Abhishek Laxminarayan Sharma
  03/2009, Degree: Master of Science by Reseasrch, Supervisor: Dr. Sunita Shailajan
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  Article: Pharmacognostical studies on the leaf of Annona squamosa Linn.

  ABHISHEK SHARMA, Vd. NAMRATA P. GORAY
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  ABSTRACT: The present communication deals with the macroscopical, microscopical & preliminary phytochemical studies on the leaf of Annona squamosa Linn. Annona squamosa Linn is used for the treatment of various diseases & ailments viz. stimulant, antispasmodic, sudorific, anthelmintic and insecticidal properties etc. No reports are available on the pharmacognostic nature of the leaf, hence, the present study was undertaken to investigate the same. All the parameters were studied according to the WHO & Pharmacopoeial guidelines. The qualitative phytochemical fingerprint of the methanolic extract revealed the presence of alkaloids, terpenoids and phenolics, fats and waxes. The aqueous leaf slurry was found to be safe at the dose level of 2g/kg body weight of mice.
  PHARMACOGNOSY JOURNAL. 01/2009; 1:88-93.
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  Article: A COMPARITIVE OF PHYTOCHEMICAL FINGERPRINT OF ASTERACANTHA LONGOFOLIA NEES. USING HPTLC

  SUNITA SHAILAJAN, ABHISHEK SHARMA
  ASIAN JOURNAL OF PLANT SCIENCES. 01/2008; 7:611-614.