Abhijit G. Banerjee

Publications (21) View all

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  Available from: Abhijit G. Banerjee

  Article: Knockdown of aberrantly expressed nuclear localized decorin attenuates tumour angiogenesis related mediators in oral cancer progression model in vitro.

  Nyla Dil, Abhijit G Banerjee
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  ABSTRACT: Oral cancer accounts for roughly 3% of cancer cases in the world with about 350,000 newly reported cases annually and a 5-year survival rate of only 50%. Majority of oral cancers are squamous cell carcinomas that originate in the oral mucosal epithelial linings. We have previously shown that in human malignant squamous cells carcinoma (SCC-25) as well as in dysplastic oral keratinocytes (DOK), a small leucine-rich multifunctional proteoglycan decorin is aberrantly expressed and localized in the nucleus where it interacts with nuclear epidermal growth factor receptor (EGFR). Post-transcriptional silencing of nuclear decorin significantly reduced IL-8 and IL8-dependent migration and invasion in these dysplastic and malignant oral epithelia. The objective of this study was to further examine the effects of nuclear decorin silencing on angiogenesis and angiogenesis related mediators in this oral cancer progression cell line model. We have used multiplex PCR, western blotting, and in vitro endothelial tube formation assay to study angiogenesis and related pathways in nuclear decorin silenced (stable knockdown) DOK and SCC-25 cells. Nuclear decorin knockdown resulted in significant down regulation of IL-8 expression, however IL-10, and TGF-β expression was not affected in either DOK or SCC25 cells as measured by multiplex RT PCR. IL-8 receptor CXCR 1 and 2 expression was slightly lower in nuclear decorin silenced cells indicating a contributing mechanism in previously shown reduced IL-8 mediated migration and invasion phenotype in these cells. IL-8 is known to induce Matrix metalloproteinase 9 (MMP9) which not only plays a role in tumour migration and invasion but also induces angiogenic switch. We found MMP9 to be significantly reduced in nuclear decorin silenced dysplastic and malignant oral epithelia. Other potent angiogenic mediators, VEGF189 and ANG-1 were either significantly reduced or completely abrogated in these cells. Angiogenesis as measured by endothelial tube-like formations of HUVEC cells was reduced by almost 50 percent when HUVECs were incubated in the presence of conditioned medium form nuclear decorin silenced dysplastic and malignant cell lines as compared to respective controls. Together these results indicate that aberrantly expressed nuclear localized decorin strongly influences angiogenic potential of dysplastic and malignant oral epithelial cells.
  Head & Neck Oncology 04/2012; 4:11. · 3.13 Impact Factor
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  Available from: Abhijit G. Banerjee

  Article: A role for aberrantly expressed nuclear localized decorin in migration and invasion of dysplastic and malignant oral epithelial cells.

  Nyla Dil, Abhijit G Banerjee
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  ABSTRACT: Oral cancer is the sixth most common malignancy worldwide with a mortality rate that is higher than many other cancers. Death usually occurs as a result of local invasion and regional lymph node metastases. Decorin is a multifunctional proteoglycan of the extracellular matrix that affects the biology of various types of cancer. Previously; we have shown that decorin is aberrantly expressed in the nucleus in human dysplastic oral keratinocytes (DOK) and malignant squamous cells carcinoma (SCC-25) and human biopsy tissues. In this study, we examined the role of nuclear decorin in oral cancer progression. We have used a post-transcriptional gene silencing (RNA interference) approach to stably knockdown nuclear decorin gene expression in DOK and SCC-25 cells using a specific shRNA plasmid and a combination of immunological and molecular techniques to study nuclear decorin function in these oral epithelial cell lines. More than 80% decorin silencing/knockdown was achieved as confirmed by real time PCR and western blot analysis in both DOK and SCC-25 cells. This RNA interference-mediated knockdown of nuclear decorin expression resulted in significantly reduced invasion and migration in these cell lines as measured by Matrigel™ coated and uncoated Trans well chamber assays respectively. Decorin silencing also resulted in reduced IL-8 mRNA and proteins levels in these cell lines. Culturing decorin silenced DOK and SCC-25 cells, with recombinant human IL-8 or IL-8 containing conditioned medium from respective un-transfected cells for 24 h prior to migration and invasion experiments, resulted in the salvation of reduced migration and invasion phenotype. Furthermore, we found that nuclear localized decorin interacts with EGFR in the nuclear fractions of both DOK and SCC-25 cells. Interestingly, EGFR (trans) activation has previously been shown to be involved in IL-8 production in various epithelia. Taken together, our results indicate that nuclear localized decorin plays an important role in migration and invasion of oral cancer cells and thus may present as a novel potential target for the treatment of oral cancer.
  Head & Neck Oncology 09/2011; 3:44. · 3.13 Impact Factor
• Chapter: Gene Expression Profiling in Gynecological Cancers

  Nyla Dil, Abhijit G. Banerjee
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  ABSTRACT: Inherited or acquired genetic mutations contribute to the pathogenesis of gynecological malignancies. Improved molecular techniques have lead to the identification of many of these genetic defects. The advent of DNA-microarray technology has permitted comprehensive genetic profiling of gynecological cancers by allowing the simultaneous study of tens of thousands of genes. Through the use of this technology, multi-gene signatures have been discovered that can highlight the mechanisms of cell growth, classify tumor histological subtypes, predict clinical end points, and also provide mechanistic information on drug resistance in gynecological cancers with important implications for developing screening tests and prognostic markers. This review discusses gene expression profiling studies of ovarian, endometrial and cervical cancers. In addition, similarities in identification of potential genetic targets, between human papilloma virus (HPV) mediated cervical cancer and head and neck cancer progression are discussed. Applications of these expression profiles may lead to novel targeted gene therapies that are critical to genetic defects seen in gynecologic cancers.
  01/2011: pages 65-80; , ISBN: 9788130804422
• Patent: Decorin polypeptide and methods and compositions of use thereof

  Abhijit G. Banerjee, Nyla Dil
  Ref. No: US20100273180, Year: 10/2010
• Patent: Product and process to regulate a gene network involved in constitutive inflammation and early cancer progression

  Abhijit G. Banerjee, Rajinder P. Bhullar
  Ref. No: US20090181091, Year: 06/2009