Abdullah Makhzoum

Questions and Answers (5) View all

• Answer added in DNA Binding
  8 Looking for software that can find DNA binding motifs in cis-regulatory regions across the whole genome
  By Kim van der Linde · Florida State University
  Abdullah Makhzoum · The University of Western Ontario

  It does matter because motif identification software and databases are created based on specific organisms so plant regulatory motifs have completely ... [more]

  It does matter because motif identification software and databases are created based on specific organisms so plant regulatory motifs have completely different databases than other organisms like softberry, transfac, etc
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• Answer added in DNA Binding
  8 Looking for software that can find DNA binding motifs in cis-regulatory regions across the whole genome
  By Kim van der Linde · Florida State University
  Abdullah Makhzoum · The University of Western Ontario

  Which organism are you working on? 

  Which organism are you working on?
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• Answer added in Vector Design
  16 After digesting the vector and removing the cDNA of our gene of interest, will the vector be empty or do we need to sequence it to confirm this?
  By Govinda Lenka · National Taipei University of Technology
  Abdullah Makhzoum · The University of Western Ontario

  Hello, This is the answer to your question: Not all plasmid copies will be digested so you need to run a gel and extract the bad according to the vect... [more]

  Hello, This is the answer to your question: Not all plasmid copies will be digested so you need to run a gel and extract the bad according to the vector size without ur cDNA or if you cut with the same restriction site, you can ligate and transform in E. coli and after extract and check size and better to sequence
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• Answer added in Transformation
  5 I am using "Biolistic transformation (particle bombardment)" to transform Tetrahymena cells. Any suggestions to improve transformation efficiency?
  By Syed Shah · Ryerson University
  Abdullah Makhzoum · The University of Western Ontario

  Hello Sayed, I am not sure that you really need biolostis technique to transform your protozoan as biolistic is very aggressive technique while you ca... [more]

  Hello Sayed, I am not sure that you really need biolostis technique to transform your protozoan as biolistic is very aggressive technique while you can try electroporation which could be softer on the death ratio of your cells.
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