Other
-
LanguagesEnglish French Arabic
Questions and Answers (5) View all
-
Answer added in DNA Binding8 Looking for software that can find DNA binding motifs in cis-regulatory regions across the whole genomeBy Kim van der Linde · Florida State UniversityAbdullah Makhzoum · The University of Western OntarioIt does matter because motif identification software and databases are created based on specific organisms so plant regulatory motifs have completely ... [more]It does matter because motif identification software and databases are created based on specific organisms so plant regulatory motifs have completely different databases than other organisms like softberry, transfac, etcFollowing
-
Answer added in DNA Binding8 Looking for software that can find DNA binding motifs in cis-regulatory regions across the whole genomeBy Kim van der Linde · Florida State UniversityAbdullah Makhzoum · The University of Western OntarioWhich organism are you working on?Which organism are you working on?Following
-
Answer added in Vector Design16 After digesting the vector and removing the cDNA of our gene of interest, will the vector be empty or do we need to sequence it to confirm this?By Govinda Lenka · National Taipei University of TechnologyAbdullah Makhzoum · The University of Western OntarioHello, This is the answer to your question: Not all plasmid copies will be digested so you need to run a gel and extract the bad according to the vect... [more]Hello, This is the answer to your question: Not all plasmid copies will be digested so you need to run a gel and extract the bad according to the vector size without ur cDNA or if you cut with the same restriction site, you can ligate and transform in E. coli and after extract and check size and better to sequenceFollowing
-
Answer added in Transformation5 I am using "Biolistic transformation (particle bombardment)" to transform Tetrahymena cells. Any suggestions to improve transformation efficiency?By Syed Shah · Ryerson UniversityAbdullah Makhzoum · The University of Western OntarioHello Sayed, I am not sure that you really need biolostis technique to transform your protozoan as biolistic is very aggressive technique while you ca... [more]Hello Sayed, I am not sure that you really need biolostis technique to transform your protozoan as biolistic is very aggressive technique while you can try electroporation which could be softer on the death ratio of your cells.Following
Publications (2) View all
-
Chapter: Hairy Roots: An Ideal Platform for Transgenic Plant Production and Other Promising Applications
09/2012; , ISBN: ISBN 978-1-4614-4065-9 -
Article: Functional analysis of the DAT gene promoter using transient Catharanthus roseus and stable Nicotiana tabacum transformation systems.
[show abstract] [hide abstract]
ABSTRACT: The Catharanthus roseus DAT gene encodes the enzyme acetyl-CoA:deacetylvindoline-4-O-acetyltransferase involved in the last step of the indole alkaloid pathway leading to vindoline. This gene is characterized by specific cell type expression in idioblasts and laticifers. To understand the specific transcriptional regulation mechanism(s) of DAT, several DAT promoter GUS constructs were cloned into pCAMBIA1305.1. Agroinfiltration of different explant types of C. roseus resulted in organ-specific accumulation of GUS, albeit at various levels. Heterologous accumulation of GUS in transgenic tobacco revealed both general and non-specific expression with the exception of a stomata-specific expression when 2.3 kb of the DAT promoter was coupled with a portion of the DAT ORF. These results suggest that in addition to the 2.3 kb upstream of the DAT transcriptional start site, additional cis-acting elements may be responsible for the specific spatial expression of DAT in vivo. Furthermore, hairy roots transformed with DAT promoter GUS constructs demonstrated GUS expression in root tissues (visualized through GUS enzyme activity), even though DAT is repressed in non-transformed roots.Plant Cell Reports 02/2011; 30(7):1173-82. · 2.27 Impact Factor