Abdul Hameed

Publications

• 1.30

  Impact points

  Molecular analysis of lipoid proteinosis: identification of a novel nonsense mutation in the ECM1 gene in a Pakistani family.

  Muhammad Nasir, Amir Latif, Muhammad Ajmal, Reem Qamar, Muhammad Naeem, Abdul Hameed

  Diagnostic pathology. 01/2011; 6:69.

  Lipoid proteinosis is a rare autosomal recessive disease characterized by cutaneous and mucosal lesions and hoarseness appearing in early childhood that is caused by homozygous or compound heterozygous mutations in the ECM1 gene located on chromosome 1q21. The aim of the study was to investigate the... [more] Lipoid proteinosis is a rare autosomal recessive disease characterized by cutaneous and mucosal lesions and hoarseness appearing in early childhood that is caused by homozygous or compound heterozygous mutations in the ECM1 gene located on chromosome 1q21. The aim of the study was to investigate the molecular genetic defect underlying lipoid proteinosis in a consanguineous Pakistani family. Genotyping of seven members of the family was performed by amplifying microsatellite markers, tightly linked to the ECM1 gene. To screen for mutations in the ECM1 gene, all of its exons and splice junctions were PCR amplified from genomic DNA and analyzed by SSCP and sequenced directly in an ABI 3130 genetic analyzer. The results revealed linkage of the LP family to the ECM1 locus. Sequence analysis of the coding exons and splice junctions of the ECM1 gene revealed a novel homozygous mutation (c.616C > T) in exon 6, predicted to replace glutamine with stop codon (p.Q206X) at amino acid position 206. The finding of a novel mutation in Pakistani family extends the body of evidence that supports the importance of ECM1 gene for the development of lipoid proteinosis.
• 4.52

  Impact points

  Novel human pathological mutations. Gene symbol: ECM1. Disease: Lipoid Proteinosis.

  Abdul Hameed, Muhammmad Nasir, Muhammad Ajmal, Latif Latif

  Human genetics. 09/2009; 126(2):336.
• 4.26

  Impact points

  A novel homozygous 62-bp insertion in ECM1 causes lipoid proteinosis in a multigeneration Pakistani family.

  M Nasir, A Latif, M Ajmal, M Ismail, A Hameed

  The British journal of dermatology. 07/2009;
• 0.55

  Impact points

  A novel poly(3-hydroxybutyrate)-degrading Strepto verticillium kashmirense AF1 isolated from soil and purification of PHB-depolymerase.

  A A Shah, Fariha Hasan, A Hameed, Safia Ahmed

  Acta biologica Hungarica. 01/2009; 59(4):489-99.

  A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This organism, identified as Streptoverticillium kashmirense AF1, secreted PHB depolymerases both on solid as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon sou... [more] A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This organism, identified as Streptoverticillium kashmirense AF1, secreted PHB depolymerases both on solid as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon source. The optimum production of PHB depolymerase was observed at pH 8 and 7, at 45 degrees C, 1% substrate concentration and in the presence of lactose as an additional carbon source. The extracellular PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75. The Streptoverticillium kashmirense AF1 produced two types of PHB depolymerases having molecular weights of about 37 and 45 kDa as determined by SDS-PAGE. The difference in dry cell mass and amount of CO2 evolved in the test and control calculated gravimetrically through Sturm test indicated the degradative capabilities of Streptoverticillium kashmirense AF1.
• 1.51

  Impact points

  Reconstruction of complex chest wall defects by using polypropylene mesh and a pedicled latissimus dorsi flap: a 6-year experience.

  Abdul Hameed, Shaheen Akhtar, Ajmal Naqvi, Zahid Pervaiz

  Journal of plastic, reconstructive & aesthetic surgery : JPRAS. 07/2008; 61(6):628-35.

  BACKGROUND: Reconstruction of full thickness defects of the chest wall is controversial and presents a complicated treatment scenario for thoracic and reconstructive plastic surgeons. It requires close cooperation between the cardiothoracic and reconstructive surgeons to achieve an optimal outcome a... [more] BACKGROUND: Reconstruction of full thickness defects of the chest wall is controversial and presents a complicated treatment scenario for thoracic and reconstructive plastic surgeons. It requires close cooperation between the cardiothoracic and reconstructive surgeons to achieve an optimal outcome and reduce the incidence of complications. OBJECTIVE: The purpose of this study is to evaluate our results in patients who underwent prosthetic bony reconstruction with polypropylene mesh and pedicle latissimus dorsi flap after chest wall resection. The principles of chest wall reconstruction include: wide excision of primary chest wall tumour with macroscopically healthy margins, wound excision and debridement of necrotic devitalised and irradiated tissues, control of infection and local wound care. STUDY DESIGN: This is a descriptive study. It includes 20 patients who underwent chest wall resection due to various causes and followed by reconstruction with polypropylene mesh along with pedicled latissimus dorsi flap. PLACE AND DURATION OF STUDY: The study was conducted at the Department of Plastic and Reconstructive Surgery, Federal Postgraduate Medical Institute, Sheikh Zayed Hospital Lahore, over a period of 6 years from August 1999 to August 2005. PATIENTS AND METHODS: This study included 20 patients who underwent chest wall reconstruction using polypropylene mesh and pedicled latissimus dorsi flap from August 1999 to August 2005. Patient demographic data including age, sex, pathological diagnosis, extent and type of resection, size of defect, and outcome were recorded. All patients were followed up in our outpatients department for 1 year. RESULTS: There was a total of 20 patients, 16 males and four females. The average age was 54 years (range 44-64 years). The indications for resection were primary chest wall tumours in 13 (65%) patients, local recurrence from breast tumours in one (5%) patient, post median sternotomy in three (15%) patients and radionecrosis in three (15%) patients. Ribs along with a part of sternum were resected in 14 (70%) patients, ribs along with clavicle in two (10%) patients and ribs only in four (20%) patients. The average area of chest wall defect after resection was 16.5 x 13 cm. In all patients, skeletal defect was reconstructed with polypropylene mesh. Soft tissue coverage was provided with a pedicled latissimus dorsi flap in all cases. Three patients with a chest wall tumour developed a recurrence within 6 months. Among these three, one patient died within 8 months of follow up due to myocardial infarction. CONCLUSION: Chest wall resection and reconstruction with synthetic polypropylene mesh and local muscle flaps can be performed as a safe, effective one-stage surgical procedure for a variety of major chest wall defects.
• 0.55

  Impact points

  Purification and characterization of a mesophilic lipase from Bacillus subtilis FH5 stable at high temperature and pH.

  Fariha Hasan, A A Shah, A Hameed

  Acta biologica Hungarica. 04/2007; 58(1):115-32.

  Lipases are a class of enzymes which catalyze the hydrolysis of long-chain triglycerides. Microbial lipases are currently receiving much attention with the rapid development of enzyme technology. Bacillus subtilis FH5, isolated from tannery wastes, produced a thermostable alkalophilic lipase and was... [more] Lipases are a class of enzymes which catalyze the hydrolysis of long-chain triglycerides. Microbial lipases are currently receiving much attention with the rapid development of enzyme technology. Bacillus subtilis FH5, isolated from tannery wastes, produced a thermostable alkalophilic lipase and was purified to homogeneity as judged by SDS-PAGE. The purification steps included acetone fractionation and sequential column chromatography on DEAE-cellulose, Sephadex G-75 and adsorption chromatography on Hydroxylapatite. The results of chromatographies showed that two types of lipases were present having molecular weights approximately 62 kDa and 24 kDa, respectively. The purified enzyme was found to be 100% stable at pH 10 and about 80% residual activity was present at 60 degrees C. The enzyme was found to be stable in the presence of Mg2+, Mn2+ and Ca2+ ions. Km value was calculated as 5.05 mM and Vmax as 0.416 micromol/ml/min. Bacillus subtilis FH5 was isolated from tannery waste, therefore, enzyme is environmentally compatible for application in leather degreasing process.
• 0.83

  Impact points

  Isolation of bacillus subtilis MH-4 from soil and its potential of polypeptidic antibiotic production.

  Bushra Jamil, Fariha Hasan, A Hameed, Safia Ahmed

  Pakistan journal of pharmaceutical sciences. 02/2007; 20(1):26-31.

  The genus Bacillus produces mainly polypeptide antibiotics such as bacitracin and polymyxin. Bacillus species were isolated from soil by soil sprinkle technique. And all were screened for the production of antibiotic. Bacillus subtilis MH-4 gave the maximum antimicrobial activity so finally selected... [more] The genus Bacillus produces mainly polypeptide antibiotics such as bacitracin and polymyxin. Bacillus species were isolated from soil by soil sprinkle technique. And all were screened for the production of antibiotic. Bacillus subtilis MH-4 gave the maximum antimicrobial activity so finally selected for optimization. During optimization of culture conditions for Bacillus subtilis MH-4 best antibacterial activity was obtained at 96 hours of incubation period, at pH-8 and by using glycerol as carbon and L-glutamic acid as nitrogen source. Optimum temperature for antibiotic production was 37 degrees C. The antibiotic was confirmed to be bacitracin by paper chromatography. Antibiotic was further extracted successfully with 1-Butanol, and aqueous concentrate showed activity of 0.8 mg/ml. The antibiotic so produced was found to be narrow spectrum active against only Gram-positive bacteria.
• 0.55

  Impact points

  Bilateral suppurative parotitis in a newborn.

  Mueen Mohyud-Din, Sadia Haider, Abdul Hameed

  Journal of the College of Physicians and Surgeons--Pakistan : JCPSP. 05/2006; 16(4):301-2.

  Suppuration occurring in inflamed parotid glands is an uncommon observation in the neonatal age group. This case report describes a male neonate, 12 days of age, who presented with high grade fever, irritability, poor feeding and bilateral swelling in the parotid region. Workup showed bilateral supp... [more] Suppuration occurring in inflamed parotid glands is an uncommon observation in the neonatal age group. This case report describes a male neonate, 12 days of age, who presented with high grade fever, irritability, poor feeding and bilateral swelling in the parotid region. Workup showed bilateral suppurative parotitis. Abscess developed despite antibiotic therapy requiring surgical drainage before final recovery.
• 1.51

  Impact points

  Versatility of the sural fasciocutaneous flap in the coverage of lower third leg and hind foot defects.

  Shaheen Akhtar, A Hameed

  Journal of plastic, reconstructive & aesthetic surgery : JPRAS. 02/2006; 59(8):839-45.

  BACKGROUND: Reconstruction of soft tissue defects of the lower third of the leg, the heel and the hind foot remains a challenge. The distally based sural artery fasciocutaneous flap has been used effectively to resurface these defects. In many instances, it has obviated the need for free tissue tran... [more] BACKGROUND: Reconstruction of soft tissue defects of the lower third of the leg, the heel and the hind foot remains a challenge. The distally based sural artery fasciocutaneous flap has been used effectively to resurface these defects. In many instances, it has obviated the need for free tissue transfer. OBJECTIVE: The objective of the study is to evaluate the efficacy of reverse sural artery fasciocutaneous flap for coverage of lower third leg, posterior heel, malleoli and hind foot. STUDY DESIGN: This is a descriptive study, which was conducted on 84 patients who presented with soft tissue defects in the area of lower third leg, heel, malleoli and hind foot. PLACE AND DURATION OF STUDY: The study was conducted at department of plastic and reconstructive surgery, Federal Postgraduate Medical Institute Shaikh Zayed Hospital Lahore, over a period of 7 years from February 1997 to February 2005. PATIENTS AND METHODS: Over a period of 7 years, a total of 84 patients with Soft tissue defect of lower third leg, heel, malleoli and hind foot were included. Preoperative data, the age and sex of each patient, cause and site of defect, dimension of flap, transposition of pedicle (through a tunnel or laid open and covered with a skin graft), postoperative results and complications were recorded. All patients were followed up in out patients department for 6 months. RESULTS: Out of 84 patients, 54 were males and 30 females. Their ages ranged from 8 to 55 years with a mean of 31 years. Road traffic accidents was the cause of the defects in 53 patients, wheel spoke injury in 12 patients, trophic ulcer in five patients, osteomyelitis in five patients, marjolin ulcer in seven patients and diabetic ulcer in two patients. The site of 84 defects comprised 52 distal tibia; 20 tendo-Achillis and posterior heel defects; seven-malleolar region; three-anterior ankle and two-foot amputation stumps. The dimension of flap ranged from 5 to 15 cm in length and 4 to 12 cm in width. Postoperatively 66 flaps survived completely while marginal necrosis was seen in six patients and infection in four patients. The complete flap necrosis occurred in eight patients. There was no considerable morbidity at donor site and all patients had satisfactory functional outcome. CONCLUSIONS: The distally based superficial sural artery flap is a versatile, reliable procedure, useful in reconstruction of lower third leg, heel, malleoli and hind foot defects. The surgical technique is safe, of short duration and provides alternative to microsurgical reconstruction.
• 0.83

  Impact points

  Resistance pattern of antibiotics against clinical isolates of Staphylococcus aureus.

  Safama Abdul Sattar, Kalsoom Farzana, Abdul Hameed

  Pakistan journal of pharmaceutical sciences. 10/2005; 18(4):18-22.

  The present research was conducted to study the susceptibility and resistance pattern of Staphylococcus aureus isolates against various brands of commonly used antibiotics. A total of 50 bacterial isolates were obtained from various clinical specimens submitted to the clinical laboratory of Pakistan... [more] The present research was conducted to study the susceptibility and resistance pattern of Staphylococcus aureus isolates against various brands of commonly used antibiotics. A total of 50 bacterial isolates were obtained from various clinical specimens submitted to the clinical laboratory of Pakistan Institute of Medical Sciences Islamabad (PIMS). Out of 50 isolates 30 (60%) were identified as coagulase positive Staphylococcus aureus. These isolates were tested for susceptibility pattern 19 (63%), these isolates were resistant to penicillin, 17 (57%) to ampicillin, 19 (63%) to cloxacillin, 20 (67%) to streptomycin, 11 (37%) to clindamycin, 16 (53%) to lincomycin and 19 (63%) to neomycin. These isolates had MIC range of penicillin and lincomycin (<0.5 to > 512 microg/mL), ampicillin, cloxacillin and clindamycin (0.5 to 256 microg/mL), streptomycin (4 to >512 microg/mL) and neomycin (2 to 256 microg/mL). Clindamycin was found to be the most effective among these antibiotics tested in the study against these clinical isolates.
• 4.52

  Impact points

  Gene symbol: AIPL1. Disease: LCA4.

  S Khaliq, Aiysha Abid, Abdul Hameed, Khalid Anwar, Aisha Mohyuddin, Muhammad Ismail, S Qasim Mehdi

  Human genetics. 06/2005; 116(6):542.
• 5.75

  Impact points

  Novel association of RP1 gene mutations with autosomal recessive retinitis pigmentosa.

  S Khaliq, A Abid, M Ismail, A Hameed, A Mohyuddin, P Lall, A Aziz, K Anwar, S Q Mehdi

  Journal of medical genetics. 06/2005; 42(5):436-8.
• 4.52

  Impact points

  Investigation of the Greek ancestry of populations from northern Pakistan.

  Atika Mansoor, Kehkashan Mazhar, Shagufta Khaliq, Abdul Hameed, Sadia Rehman, Saima Siddiqi, Myrto Papaioannou, L L Cavalli-Sforza, S Qasim Mehdi, Qasim Ayub

  Human genetics. 05/2004; 114(5):484-90.

  Three populations from northern Pakistan, the Burusho, Kalash, and Pathan, claim descent from soldiers left behind by Alexander the Great after his invasion of the Indo-Pak subcontinent. In order to investigate their genetic relationships, we analyzed nine Alu insertion polymorphisms and 113 autosom... [more] Three populations from northern Pakistan, the Burusho, Kalash, and Pathan, claim descent from soldiers left behind by Alexander the Great after his invasion of the Indo-Pak subcontinent. In order to investigate their genetic relationships, we analyzed nine Alu insertion polymorphisms and 113 autosomal microsatellites in the extant Pakistani and Greek populations. Principal component, phylogenetic, and structure analyses show that the Kalash are genetically distinct, and that the Burusho and Pathan populations are genetically close to each other and the Greek population. Admixture estimates suggest a small Greek contribution to the genetic pool of the Burusho and Pathan and demonstrate that these two northern Pakistani populations share a common Indo-European gene pool that probably predates Alexander's invasion. The genetically isolated Kalash population may represent the genetic pool of ancestral Eurasian populations of Central Asia or early Indo-European nomadic pastoral tribes that became sequestered in the valleys of the Hindu Kush Mountains.

  Download
• 3.21

  Impact points

  Extended-spectrum beta-lactamases (ESbLs): characterization, epidemiology and detection.

  A A Shah, F Hasan, S Ahmed, A Hameed

  Critical reviews in microbiology. 02/2004; 30(1):25-32.

  Beta-lactamases of Enterobacteriaceae are the most important mechanism of resistance against beta-lactam drugs. Two types of beta-lactamases can confer resistance against 3rd generation cephalosporins. Chromosomally mediated beta-lactamases are inducible and are not inhibited by clavulanic acid. Res... [more] Beta-lactamases of Enterobacteriaceae are the most important mechanism of resistance against beta-lactam drugs. Two types of beta-lactamases can confer resistance against 3rd generation cephalosporins. Chromosomally mediated beta-lactamases are inducible and are not inhibited by clavulanic acid. Resistance due to these enzymes is non-transferable. The 2nd type of enzyme is plasmid-mediated beta-lactamases, which are inhibited by clavulanic acid. These enzymes are more important clinically as these can be transferred between various species of Enterobacteriaceae. These enzymes are called extended-spectrum beta-lactamases (ESBLs). ESBL-producing Enterobacteriaceae have been responsible for numerous outbreaks of infection throughout the world and pose challenging infection control issues. Antibacterial choice is often complicated by multi-resistance. ESBLs can confer resistance against all beta-lactam drugs except carbapenems and cephamycins. Nursing home patients may be an important reservoir of ESBL-containing multiple antibiotic-resistant organisms. Use of broad-spectrum oral antibiotics and probably poor infection control practices may facilitate spread of this plasmid-mediated resistance. In addition to known populations at risk, ambulatory patients with chronic conditions represent another patient population that may harbor ESBL-producing organisms. Various methods can be used for detection of ESBLs in the laboratory. These tests include double disc diffusion test, Vitek ESBL test, E Tests, MIC Determination, genetic method, and isoelectric focusing (IEF).

• Ischemic stroke subtypes in Pakistan: the Aga Khan University Stroke Data Bank.

  N A Syed, B A Khealani, S Ali, A Hasan, N Akhtar, H Brohi, T Mozaffar, N Ahmed, A Hameed, S M Baig, M Wasay

  JPMA. The Journal of the Pakistan Medical Association. 01/2004; 53(12):584-8.

  OBJECTIVE: Frequency of ischemic stroke subtypes is influenced by ethnic and geographic variables. Our objective was to identify various stroke subtypes and its determinants at a tertiary care hospital. METHODS: We prospectively collected data on ischemic stroke subtypes admitted to The Aga Khan Uni... [more] OBJECTIVE: Frequency of ischemic stroke subtypes is influenced by ethnic and geographic variables. Our objective was to identify various stroke subtypes and its determinants at a tertiary care hospital. METHODS: We prospectively collected data on ischemic stroke subtypes admitted to The Aga Khan University Hospital in Karachi. RESULTS: A total of 596 patients were enrolled in 22 months in the Aga Khan Universtiy Stroke Registry. These included 393 patients with Ischemic stroke, 126 patients with intracerebral hemorrhage, 50 patients with subarachnoid hemorrhage and others. The ischemic stroke group was classified according to the TOAST criteria and comprised of lacunar 168/393 (42.7%); large artery atherosclerosis 106/393 (26.9%); cardioembolic 24/393 (6.1%); undetermined 80/393 (20.3%); and other determined types 15/393 (3.8%). The high proportion of lacunar strokes in our population may be due to high burden of inadequately treated hypertension and diabetes. Clear cut cardioembolic stroke was relatively infrequent in our population. CONCLUSION: Lacunar stroke is the most common subtype of stroke in our patient population. This is most likely secondary to uncontrolled hypertension.
• 0.55

  Impact points

  Neonatal sepsis in hospital-born babies: bacterial isolates and antibiotic susceptibility patterns.

  Brekhna Aurangzeb, Abdul Hameed

  Journal of the College of Physicians and Surgeons--Pakistan : JCPSP. 12/2003; 13(11):629-32.

  OBJECTIVE: To determine the frequency of bacterial isolates from neonatal blood cultures and their susceptibility patterns in hospital-born babies having sepsis. DESIGN: An observational study. PLACE AND DURATION OF STUDY: Obstetrics Unit and Special Care Baby Unit of Khyber Teaching Hospital, Pesha... [more] OBJECTIVE: To determine the frequency of bacterial isolates from neonatal blood cultures and their susceptibility patterns in hospital-born babies having sepsis. DESIGN: An observational study. PLACE AND DURATION OF STUDY: Obstetrics Unit and Special Care Baby Unit of Khyber Teaching Hospital, Peshawar from 1st January to 31st December 2001. SUBJECTS AND METHODS: This study was carried out on the neonates born and admitted in hospital with positive blood culture reports. Early onset neonatal sepsis (EONNS) and late onset neonatal sepsis (LONNS) were defined as illness appearing from birth to seven days and from eight to twenty-eight days postnatal age respectively. The blood culture reports were analyzed by SPSS package and cross tabulation was done. RESULTS: One hundred and twelve hospital-born babies presented with sepsis. Sixty-seven neonates had positive cultures. Escherichia coli (E. coli) was the commonest organism causing EONNS (35; 77.1%) followed by Pseudomonas (4; 8.9%), Klebsiella (4; 8.9%) and Staphylococcus aureus (2; 4.4%) respectively. In the LONNS E. coli (19; 77.3%) was the commonest followed by Staphylococcus and Pseudomonas (2; 9% each) and Klebsiella (1; 4.5%). The gram-negative organisms showed high degree of resistance to commonly used antibiotics, ampicillin (79.3%), amoxicillin (74.6%) and ceftazidime (71.6%), cefotaxime (55.2%) and comparatively low resistance to gentamicin (43.2%), tobramycin (34.3%), imipinem (23.6%), amikacin (22.3%), ofloxacin and ciprofloxacin (11.9%) respectively. Staphylococcus aureus showed almost the same resistance to ampicillin, 75%, and comparatively low resistance to the rest of the antibiotics as compared to the gram-negative organisms. CONCLUSION: Neonatal sepsis is mainly caused by gram-negative organisms, which are developing resistance to commonly used antibiotics.
• 2.76

  Impact points

  Reconstruction of human evolutionary tree using polymorphic autosomal microsatellites.

  Qasim Ayub, Atika Mansoor, Muhammad Ismail, Shagufta Khaliq, Aisha Mohyuddin, Abdul Hameed, Kehkashan Mazhar, Sadia Rehman, Saima Siddiqi, Myrto Papaioannou, Alberto Piazza, Luigi Luca Cavalli-Sforza, S Qasim Mehdi

  American journal of physical anthropology. 12/2003; 122(3):259-68.

  Allelic frequencies of 182 tri- and tetra-autosomal microsatellites were used to examine phylogenetic relationships among 19 extant human populations. In particular, because the languages of the Basques and Hunza Burusho have been suggested to have an ancient relationship, this study sought to explo... [more] Allelic frequencies of 182 tri- and tetra-autosomal microsatellites were used to examine phylogenetic relationships among 19 extant human populations. In particular, because the languages of the Basques and Hunza Burusho have been suggested to have an ancient relationship, this study sought to explore the genetic relationship between these two major language isolate populations and to compare them with other human populations. The work presented here shows that the microsatellite allelic diversity and the number of unique alleles were highest in sub-Saharan Africans. Neighbor-joining trees based on genetic distances and principal component analyses separated populations from different continents, and are consistent with an African origin for modern humans. For the first time, with biparentally transmitted markers, the microsatellite tree also shows that the San are the first branch of the human tree before the branch leading to all other Africans. In contrast to an earlier study, these results provided no evidence of a genetic relationship among the two language isolate groups. Genetic relationships, as ascertained by these microsatellites, are dictated primarily by geographic proximity rather than by remote linguistic origin, Mantel test, R(0) = 0.484, g = 3.802 (critical g value = 1.645; P = 0.05).
• 0.55

  Impact points

  Comparative efficacy of amoxicillin, cefuroxime and clarithromycin in the treatment of community -acquired pneumonia in children.

  Brekhna Aurangzeb, Abdul Hameed

  Journal of the College of Physicians and Surgeons--Pakistan : JCPSP. 12/2003; 13(12):704-7.

  OBJECTIVE: To compare the clinical response to amoxicillin, cefuroxime and clarithromycin in the treatment of community-acquired pneumonia in children and to see the cost effectiveness of each treatment. DESIGN: Randomized clinical control trial. PLACE AND DURATION OF STUDY: Department of Pediatrics... [more] OBJECTIVE: To compare the clinical response to amoxicillin, cefuroxime and clarithromycin in the treatment of community-acquired pneumonia in children and to see the cost effectiveness of each treatment. DESIGN: Randomized clinical control trial. PLACE AND DURATION OF STUDY: Department of Pediatrics, Khyber teaching hospital, Peshawar, from October 2001 to February 2002. SUBJECTS AND METHODS: Patients between 3 to 72 months of age, admitted in the hospital with community acquired pneumonia, were randomly divided into three groups,1,2,3. They were started on amoxicillin, cefuroxime and clarithromycin respectively. The patients were assessed daily. If there was no clinical improvement at 48 hours the antibiotic was changed. ANOVA statistical test was applied to see the clinical response to the treatment in the three groups. Cost effectiveness of the treatment was compared. RESULTS: There was no statistical difference in the clinical response at 48 hours of initiating treatment and at discharge (p > 0.01 each). The mean hospital stay in group 1 and 2 was 3.3 days and group 3 was 3.2 days respectively (p > 0.01). Ninety-seven percent patients in group 1 and 3, and 95% patients in group 2 showed clinical improvement. The cost of treatment of community acquired pneumonia for 8 days was Rs 496/-, 730/-, 1018/- for amoxicillin, clarithromycin and cefuroxime respectively. CONCLUSION: Amoxicillin was found the most cost effective followed by clarithromycin and cefuroxime respectively in the treatment of non-severe and severe community-acquired pneumonia.
• 1.92

  Impact points

  Subclinical hypothyroidism: an audit of management.

  P K Prakash, H Bolusani, A Hameed, L D K E Premawardhana

  Annals of clinical biochemistry. 12/2003; 40(Pt 6):694-6.

  BACKGROUND: Subclinical hypothyroidism (SH) is a marker for overt hypothyroidism and vascular disease. Treatment guidelines are not universally followed. Thyroxine is recommended if serum thyroid-stimulating hormone (TSH) concentration is 10 mU/L or more, or if serum TSH is 5-9.9 mU/L (mild SH) with... [more] BACKGROUND: Subclinical hypothyroidism (SH) is a marker for overt hypothyroidism and vascular disease. Treatment guidelines are not universally followed. Thyroxine is recommended if serum thyroid-stimulating hormone (TSH) concentration is 10 mU/L or more, or if serum TSH is 5-9.9 mU/L (mild SH) with other risk factors, such as thyroid peroxidase antibodies (TPOAb). METHODS: We examined the management of mild SH in a retrospective case note audit of 150 consecutive subjects. Twenty-seven subjects with a serum TSH concentration above 10 mU/L were excluded from analysis. Of the group with mild SH, 27 were also excluded because of previous thyroid disease or amiodarone therapy. RESULTS: The prevalence of previous thyroid disease was similar in subjects with TSH 10 mU/L or more, compared to those with mild SH. Overall, both TPOAb and goitre status were determined in only 39% of subjects with mild SH, but in more by endocrinologists compared with general physicians (63% versus 22% for TPOAb; 47% versus 17% for goitre) (P = 0.001). Endocrinologists treated a greater number of subjects with mild SH who were eligible for thyroxine therapy compared to nonendocrine colleagues (96% versus 67%) (P = 0.024). Both groups treated subjects in whom TPOAb status was not determined (endocrinologists 21% versus general physicians 40%) (P = 0.21). CONCLUSION: In subjects with mild SH, evaluation is incomplete, a large percentage who were TPOAb positive were on appropriate therapy, thyroxine was prescribed when TPOAb status was unknown and, on the whole, endocrinologists performed better than general physicians.
• 5.75

  Impact points

  Evidence of RPGRIP1 gene mutations associated with recessive cone-rod dystrophy.

  A Hameed, A Abid, A Aziz, M Ismail, S Q Mehdi, S Khaliq

  Journal of medical genetics. 09/2003; 40(8):616-9.