@saicharan - do we have any cell culture medium available with this HEPES buffer?
@ Chandrashekaram Kans- Hyclone make DMEM media is available with HEPES Buffer
Any one here can have comment on new designed incubator with Tri gases; nitrogen, CO2 and Oxygen plus controlled %RH? As far as I know, N2 gas used to control oxygen level in incubator, by doing this one can set his incubator to hypoxic condition reducing oxygen level to less than 5%.
I wonder if old fashion incubator having only CO2 monitor will be good enough for cell growth comparing with new designed which have %RH and oxygen level monitored?
Khalid Omer Alfarouk
University of Khartoum
Indian Institute of Technology Bombay
University of Leipzig
Chandrasekaram Kans 430-2100
Hanan mohammed kamal Abd el fatah Osman
National Institute of Oceanography
Mahdi Habibpour Fatideh
Amirkabir University of Technology
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