Question

What is the most famous gene whose transcript levels are regulated by ERK?

I want to know whether or not the gene of my interest is sensitive to PD98059 (MEK/ERK inhibitor) and I have this distinct feeling that it is not. Even if the gene was indeed insensitive to PD98059, I still have to check other genes regulated by ERK to see if my PD98059 was really working. I know the most credible way to do this is to do the Western blot analysis of phospho-ERK, but the easiest way is RT-PCR and I heard c-Fos is regulated by ERK. Can someone please tell me other genes regulated by ERK?

Apr 14, 2012

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Fahd Al-Mulla · Kuwait University

You need to be careful with this. As ERK1/2 RESPONSIVE GENES may be cell specific and also ossilate. So it really depends on the cell type you are working with. But if you want the most common, may be they are the cyclins. Again be careful with these as they are also controlled post-transcriptionally by other molecules like GSK3b etc. I hope this helps

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You need to be careful with this. As ERK1/2 RESPONSIVE GENES may be cell specific and also ossilate. So it really depends on the cell type you are working with. But if you want the most common, may be they are the cyclins. Again be careful with these as they are also controlled post-transcriptionally by other molecules like GSK3b etc.

I hope this helps

Apr 14, 2012
Zhong Yao · University of Toronto

You have to check ERK1/2 phosphorylation if you want your results acceptable to the field.

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You have to check ERK1/2 phosphorylation if you want your results acceptable to the field.

Apr 14, 2012
Carlos Guillen ·

Hi. I prefer to use U0126 inhibitor for blocking ERK signaling. Yes, one of the most important genes regulated by ERKs is c-fos, as a component of AP1 transcription factor. However, there are a lot of genes regulated by ERKs. I suggest to you read the next paper http://www.biochemj.org/bj/441/0553/bj4410553.htm

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Hi. I prefer to use U0126 inhibitor for blocking ERK signaling. Yes, one of the most important genes regulated by ERKs is c-fos, as a component of AP1 transcription factor. However, there are a lot of genes regulated by ERKs. I suggest to you read the next paper
http://www.biochemj.org/bj/441/0553/bj4410553.htm

Apr 14, 2012
Gabriella Castoria · Second University of Naples

Hi. There are a lot of genes regulated by activated ERK1/2. They include cyclins, cell cycle inhibitors, Fos and many others. The results depend on a plaetora of factors, including the type of ligand, the amplitude of signal, the cell environment. However, I suggest you to read the papers of M. Cobb and C. Marshall.

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Hi. There are a lot of genes regulated by activated ERK1/2. They include cyclins, cell cycle inhibitors, Fos and many others.
The results depend on a plaetora of factors, including the type of ligand, the amplitude of signal, the cell environment. However, I suggest you to read the papers of M. Cobb and C. Marshall.

Apr 14, 2012
Tony Lefebvre · Université des Sciences et Technologies de Lille 1

Hi all. You can also look at Rsk, easy to to check using phospho-dependent antibodies. Best. Tony

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Hi all.
You can also look at Rsk, easy to to check using phospho-dependent antibodies.
Best.
Tony

Apr 14, 2012
Martin Mcmahon · University of California, San Francisco

MYC, HB-EGF, Cyclin D1, p21Cip1, p16INK4a, DUSP4/6, FOS, JUN, Sproutys to name but a few but beware of cell type specific differences.

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MYC, HB-EGF, Cyclin D1, p21Cip1, p16INK4a, DUSP4/6, FOS, JUN, Sproutys to name but a few but beware of cell type specific differences.

Apr 14, 2012
Hanaka Mimura · Chiba University

Thank you very much to you all. It really helps!!

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Thank you very much to you all. It really helps!!

Apr 15, 2012
Eusebio Perdiguero · University Pompeu Fabra

I agree with Zhong Yao. Even if you detect changes in expression, you will have to check Phospho-ERK levels in any case.

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I agree with Zhong Yao. Even if you detect changes in expression, you will have to check Phospho-ERK levels in any case.

Apr 16, 2012
Ghulam Mohammad · Wayne State University

MMPs are regulated by ERK, RT-PCR never tell you the activation, you have to do western blot using Phospho-ERK and ERK antibody

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MMPs are regulated by ERK, RT-PCR never tell you the activation, you have to do western blot using Phospho-ERK and ERK antibody

Apr 24, 2012
Gabriela Gorelik · University of Michigan

Those are different things. To check if your inhibitor is working, you need to measure p-ERK by WB. And regarding the genes that are regulated by ERK, there are many. ERK activates AP1 transcriptor factor that regulates different genes, as i.e. Dnmt. So, you need to do RT-PCR to measure the expression of your specific gene after the incubation with the inhibitor.

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Those are different things. To check if your inhibitor is working, you need to measure p-ERK by WB. And regarding the genes that are regulated by ERK, there are many. ERK activates AP1 transcriptor factor that regulates different genes, as i.e. Dnmt. So, you need to do RT-PCR to measure the expression of your specific gene after the incubation with the inhibitor.

May 10, 2012
Evgenia Gerasimovskaya · University of Colorado Denver

Egr-1 is a very sensitive target for ERK pathway. Also, it is a master-regulator of many GF genes, tissue factor, etc. I have a probe for non-radioactive NB.

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Egr-1 is a very sensitive target for ERK pathway. Also, it is a master-regulator of many GF genes, tissue factor, etc. I have a probe for non-radioactive NB.

Aug 27, 2012