I think you cannot really use a specific formula for enzyme activity. As pointed out earlier, the activity is measured over time. It may be better to have a plot of absorbance as a function of time, then compare this with a standard curve of a known enzyme standard. Hope this can help.
Absorbance value use in the standard curve and determine the PNP concentration, then, for example, beta-galactosidase activity (U) = PNP conentration x total reaction volume/ reaction time x enzyme volume
hello dear akalesh
I performed catalse activity in brain tissue. The reaction mixture contain buffer tissue homogenate (100ul) and different concentartion of hydrogen peroxide. I observed absorbance change then how can i get result enzyme activity. Can i represent it in unit per mg of protein. And can i also obtain Km and Vmax value through this experiments.
Universidad Nacional de Mar del Plata
Mohammad Mizanur Rahman
National Environmental Engineering Research Institute
Akalesh Kumar Verma
Cachar Cancer Hospital & Research Centre
University of Khartoum
Uma Kant Sharma
University of Allahabad
Sardar Patel University