What is the best method for dissecting anatomically specific rat brain tissue?
I'm trying to isolate striatum, PFC, and amygdala from whole brain tissue from Long-Evans rats in a uniform manner (for use in a ChIP and/or immunoblot procedure(s)). I've tried doing this free-hand and worry about the consistency among samples. Does anyone have any experience with using cryostat sections and then punching out specific regions? Are there any other techniques that would perhaps be better??