Question

What are the different methods to prevent proteus swarming on any culture media?

Swarmming of proteus on the whole agar plate.
May 28, 2012

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All Answers (34)

  • Ana María Sánchez Peralta · University of Granada
    Agar more concentrate, impedes its progress. And with deoxycholate (deoxycholate agar).
    May 28, 2012
  • Sowmiya Murali · Sankara Nethralaya
    Agar concentration has to be increased to atleast 4 % or the good choice is grow in Macconkey agar...... You can surely prevent swarming
    May 30, 2012
  • Shagufta Liaqat · University of Health Sciences
    Can it be prevented on CLED medium??????
    May 30, 2012
  • Sowmiya Murali · Sankara Nethralaya
    Thats also good idea of Shagufta Liaqat ....... If it is available it can be used... but all labs will surely have MacConkey agar.. Thats y i suggested
    May 30, 2012
  • Ana María Sánchez Peralta · University of Granada
    @Shagufta YES.
    May 30, 2012
  • Shagufta Liaqat · University of Health Sciences
    but in my experience, proteus swarms on MacConkey agar.... then usualy i need to use CLED medium...
    May 31, 2012
  • Sowmiya Murali · Sankara Nethralaya
    oh ok when i worked with Proteus i usually use even nutrient agar or any basal medium with 4 % agar concentration....... However CLED is off course good choice.............
    May 31, 2012
  • Md Shahbaz Anwar · G. B. Pant University of Agriculture and Technology
    I think CLED medium will best
    Jun 2, 2012
  • Vijay Kumar · JSS University
    Routine Chrom Agar Medium (Urichrome) helps in identification and prevents swarming.
    Jun 2, 2012
  • Ersilia Fiscarelli · Ospedale Pediatrico Bambino Gesù
    I agree with SHAHBAZ Cled IS A GOOD CHOICE...
    Jun 4, 2012
  • Ole Heltberg · Slagelse Hospital
    Here is another very simple method, that works well in fx blood agar or chocolate agar plates: Take the normal plate You intend to use, pour conc. ethanol (95 or 99%) over the plate - flood it. Let it stand say 0,5 min,, then drain the plate, and let the alcohol damp off several minutes, without cover. Your agarplate is then ready for seeding, susceptinility testing etc. without, -- or with very limited -- swarming :-)
    Jun 4, 2012
  • Shagufta Liaqat · University of Health Sciences
    @ Heltberg... ohh realy? dats great.... cozz proteus causes alot of problem on blood nd choclate agar.. dats a great idea, thanx :-)
    Jun 5, 2012
  • Miryana Issa
    1 drop of metanol on cover agar plate inhibite swarming of Proteus.
    Jun 5, 2012
  • Ramana k v · Prathima Institute of Medical Sciences
    I used 40%-70% alcohol pls try
    Jun 5, 2012
  • Mukund Bodhankar · Bharati Vidyapeeth Deemed University
    Just grow on 3% agar.
    Jun 6, 2012
  • Shagufta Liaqat · University of Health Sciences
    Thanx alot to all of u 4 sharing so much diffeernt methods.....:-)
    Jun 7, 2012
  • Zhou Zhaoyan · Fudan University
    using Mac plate will be good
    Jun 8, 2012
  • Mohamed Emara · Helwan University
    Alcohol is the best in my opinion
    Jun 10, 2012
  • Giovanni Tampus · Xavier University - Ateneo de Cagayan
    Adding alcohol on the agar plate might do the trick but it will also dehydrate your agar at a faster rate than the ordinary. If you are using a mixed culture then definitely Proteus will be all over your plate. It would be best to isolate your organism of interest using a selective medium which will inhibit the growth of Proteus.
    Jun 13, 2012
  • Brian David
    Reduce the amount of salt and increase the amount of agar. Mac plates work best if they have reduced salt content, which is why cled is also usefull
    Jun 16, 2012
  • Gavin Koh · Heart of England NHS Foundation Trust
    Electrolyte deficient plates inhibit swarming. That is the usual way to inhibit swarming
    Jun 18, 2012
  • Shri Natrajan
    Swarming can be inhibited by 0.5 to 1.25% of Urea
    Jun 18, 2012
  • Paola Scavone · Clemente Estable Biological Research Institute
    You can use Nutrient Agar check that it has not NaCl, or CLED agar that is specific for swarming inhibition.
    Jun 18, 2012
  • Vijay Kumar · JSS University
    Increase the concentration of agar, that is the best and easy method to prevent swarming
    Jun 18, 2012
  • Adugna Abera
    Just use electrone defficint media such as CLED
    Jun 19, 2012
  • Iriah Samuel · Medical Laboratory Science Council of Nigeria
    CLED is it!
    Jun 20, 2012
  • Mujibur Rahman · Rangpur Medical College
    Mac agar can be used to serve the purpose
    Jun 27, 2012
  • Michael Rowe · Agri-Food and Biosciences Institute
    Simply increase concentration of agar in the medium.
    Jul 6, 2012
  • Augustine Ssevviri · Makerere University
    Bile salts are the conventional way of inhibiting swarming of Proteus. I just don't know whether this has not worked for you at all but I believe this from my long term experience it should work. Try Bile salt containing media
    Jul 9, 2012
  • Augustine Ssevviri · Makerere University
    Indeed electrolyte deficient media agar inhibit swarming of Proteus; CLED is.and it manifests with clearly blue to blue green translucent colonies as an indication of its failure to ferment lactose so incorporated. However, the argument to increase agar concentrations in media should be handled a little more carefully as to put into consideration the ambient organism growth conditions for the best isolation and identification of the various strains.
    Jul 10, 2012
  • Vijay Kumar · JSS University
    I have already replied, it is high time this discussion is deleted
    Jul 10, 2012
  • Manfred Rotter · Medical University of Vienna
    Add 0.05% Pril to liquefied agar
    Jul 12, 2012
  • Vijay Kumar · JSS University
    Increasing the agar concentration is the best and easiest method
    Jul 12, 2012

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